Video: Dab tsi yog lub sijhawm txawb hauv nyias txheej chromatography?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Dab tsi yog qhov mobile theem nyob rau hauv nyias txheej chromatography ? Cov mobile theem yog ib tug haum kua hnyav los yog sib tov ntawm cov kuab tshuaj. Cov mobile theem ntws los ntawm qhov chaw nres tsheb theem thiab nqa cov khoom sib xyaw nrog nws. Cov khoom sib txawv taug kev ntawm tus nqi sib txawv.
Hais txog qhov no, lub sijhawm txawb hauv TLC yog dab tsi?
Lub silica gel (los yog alumina) yog nyob ruaj ruaj theem . Qhov chaw nres tsheb theem rau nyias txheej chromatography kuj feem ntau muaj cov khoom uas fluoresces hauv UV teeb - vim li cas koj yuav pom tom qab. Cov mobile theem yog cov kua dej haum kuab tshuaj los yog sib tov ntawm cov kuab tshuaj.
Tsis tas li ntawd, nyias txheej chromatography cais cov tebchaw li cas? Nyias txheej chromatography , los yog TLC, yog ib txoj hauv kev los tshuaj xyuas cov khoom sib tov los ntawm kev sib cais cov tshuaj sib xyaw hauv qhov sib tov. Kev loj hlob yog muab tso rau hauv qab ntawm TLC phaj rau hauv lub pas dej ntiav ntiav ntawm ib qho kev loj hlob hnyav, uas tom qab ntawd mus txog lub phaj los ntawm kev ua haujlwm capillary.
Qhov thib ob, lub luag haujlwm ntawm lub sijhawm txawb hauv ib txheej nyias thiab daim ntawv chromatography yog dab tsi?
Nyias - txheej chromatography (TLC) yog a chromatography txheej txheem siv los cais cov tsis-volatile sib tov. Tom qab cov qauv tau thov rau ntawm lub phaj, ib qho hnyav lossis hnyav sib tov (hu ua mobile theem ) yog kos lub phaj los ntawm kev ua haujlwm capillary.
Lub hauv paus ntsiab lus ntawm nyias txheej chromatography yog dab tsi?
Chromatography ua haujlwm ntawm txoj cai uas sib txawv tebchaw yuav muaj sib txawv solubilities thiab adsorption rau ob theem ntawm uas lawv yuav tsum tau muab faib. Nyias Txheej Chromatography ( TLC ) yog cov txheej txheem ua kua uas ob theem yog cov khoom (stationary phase) thiab cov kua (qib txav).
Pom zoo:
Puas yog glycerol xav tau daim nyias nyias nyias kom hla daim nyias nyias?
Glycerol yog lipid soluble yog li nws diffuses los ntawm yooj yim diffusion ncaj qha los ntawm lub cell membrane thaum cov piam thaj yog ib tug polar molecule yog li nws diffuses los ntawm kev yooj yim diffusion uas txhais tau hais tias nws xav tau ib tug channel protein ua hauj lwm thiab qhov no txhais tau tias thaj chaw saum npoo rau cov piam thaj kom nkag mus yog tsawg dua. tshaj ib qho rau glycerol
Cov txheej nyias nyias chromatography yog dab tsi thiab nws ua haujlwm li cas?
Nyias-txheej chromatography (TLC) yog ib qho txheej txheem chromatography siv los cais cov khoom sib xyaw uas tsis yog-volatile. Tom qab cov qauv tau siv rau ntawm lub phaj, cov kuab tshuaj los yog cov kuab tshuaj sib tov (lub npe hu ua lub sijhawm txawb) yog kos rau ntawm lub phaj ntawm capillary nkaus
Dab tsi yog qhov chaw nyob ruaj khov nyob rau hauv nyias txheej chromatography?
Lub silica gel (los yog alumina) yog qhov chaw nres tsheb. Cov theem nyob ruaj khov rau nyias txheej txheej chromatography kuj feem ntau muaj cov khoom siv fluorescesin UV teeb - vim li cas koj yuav pom tom qab. Lub mobilephase yog cov kuab tshuaj uas tsim nyog los yog sib tov ntawm cov kuab tshuaj
Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?
Rau silica gel-coated TLC daim hlau, lub zog eluent nce hauv qhov kev txiav txim hauv qab no: perfluoroalkane (tsis muaj zog), hexane, pentane, carbon tetrachloride, benzene / toluene, dichloromethane, diethyl ether, ethyl acetate, acetonitrile, acetone / 2-propylene. -butanol, dej, methanol, triethylamine, acetic acid, formic acid
Lub hauv paus ntsiab lus ntawm nyias txheej chromatography yog dab tsi?
Chromatography ua haujlwm ntawm lub hauv paus ntsiab lus uas sib txawv tebchaw yuav muaj kev sib txawv solubilities thiab adsorption rau ob theem ntawm lawv yuav tsum tau muab faib. Thin Layer Chromatography (TLC) yog cov txheej txheem ua kua uas muaj ob theem yog cov khoom (stationary phase) thiab cov kua (qib txav mus)