Video: Dab tsi yog qhov chaw nyob ruaj khov nyob rau hauv nyias txheej chromatography?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov silica gel (los yog alumina) yog cov nyob ruaj ruaj . Cov nyob ruaj khov theem rau nyias layerchromatography kuj feem ntau muaj ib yam khoom uas fluorescesin UV teeb - vim li cas koj yuav pom tom qab. Cov mobilephase yog ib tug haum kua hnyav los yog sib tov ntawm cov kuab tshuaj.
Tsis tas li ntawd, dab tsi yog qhov chaw nres tsheb hauv chromatography?
Chromatography yog siv los cais cov khoom sib tov rau hauv lawv cov khoom. Lawv txhua tus muaj ib tug nyob ruaj ruaj (ib qho khoom, lossis cov kua dej txhawb rau ntawm cov khoom) thiab a mobile theem (ua kua los yog roj). Cov mobile theem ntws los ntawm nyob ruaj khov theem thiab nqa cov khoom ntawm qhov sib tov nrog nws.
Qhov thib ob, cov ntaub ntawv dab tsi yog siv nyob rau hauv qhov chaw nres tsheb rau TLC phaj? Cov silica gel tseem yog qhov tseem ceeb tshaj plaws adsorbent rau TLC kev sib cais. Lub kinetic zog ntawm silica-gelthin txheej thiab cov tshiab TLC daim hlau tau nthuav tawm. Lwm yam cov ntaub ntawv siv raws li nyob ruaj khov theem yog alumina, zirconium oxide, Florisil, thiab ion-exchanger.
Ntawm no, dab tsi yog theem mobile hauv TLC?
Cov mobile theem yog cov kua dej haum kuab tshuaj los yog sib tov ntawm cov kuab tshuaj. Confinement ntawm a TLC phaj nyob rau hauv lub chamber uas muaj nws lub taub hau-qhov chaw (cov huab cua nyob rau hauv lub chamber) saturated nrog kuab tshuaj vapor tso cai rau elution ntawm tus qauv los ntawm kev ua capillary.
Lub hauv paus ntsiab lus ntawm nyias txheej chromatography yog dab tsi?
Chromatography ua haujlwm ntawm txoj cai uas txawv tebchaw yuav muaj sib txawv solubilities thiab adsorption rau ob theem ntawm uas lawv yuav tsum tau muab faib. ThinLayer Chromatography ( TLC ) yog cov txheej txheem ua kua uas ob theem yog cov khoom (stationary phase) thiab cov kua (qib txav).
Pom zoo:
Puas yog glycerol xav tau daim nyias nyias nyias kom hla daim nyias nyias?
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Cov txheej nyias nyias chromatography yog dab tsi thiab nws ua haujlwm li cas?
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