Video: Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Rau silica gel-coated TLC Daim hlau, lub zog eluent nce hauv qhov kev txiav txim hauv qab no: perfluoroalkane (tsis muaj zog), hexane, pentane, carbon tetrachloride, benzene / toluene, dichloromethane, diethyl ether, ethyl acetate, acetonitrile, acetone, 2-propanol / n-butanol, dej,, triethylamine, acetic acid, formic acid
Tsuas yog li ntawd, vim li cas thiaj li sib xyaw cov kuab tshuaj siv hauv chromatography?
1 Teb. Cov kuab tshuaj yog siv los pab cais cov Cheebtsam ntawm a sib tov . Cov solute xaiv yuav tsum muaj peev xwm yaj cov khoom ntawm cov sib tov . Ntawm no yog ib daim vis dis aus ntawm kev sim ua los cais cov khoom ntawm cov dej soluble number case.
Ib sab saum toj no, vim li cas ethyl acetate yog cov kuab tshuaj zoo rau TLC? Yog hais tias tus polar attraction ntawm tus qauv thiab silica ntau dua tus qauv yuav nyob ntau dua nyob rau hauv silica es eluting. Yog li ntawd yog hais tias tus kuab tshuaj muaj ntau polarity nws zoo dua. Polarity ntawm ethyl acetate hmoov yog ntau tshaj 4 uas siab tshaj ntau lwm yam kuab tshuaj thiab yog vim li cas ethyl acetate hmoov yog siv.
Tsis tas li ntawd, cov kuab tshuaj cuam tshuam li cas TLC?
Lub zog eluting ntawm cov kuab tshuaj nce nrog polarity. Yog li ntawd, tsis tshua muaj polarity tebchaw yuav eluted nrog tsawg polarity cov kuab tshuaj , thaum cov polarity ntau dua xav tau cov kuab tshuaj ntawm polarity siab dua. Lub zog muaj zog yog khi rau lub adsorbent, qeeb qeeb nws txav mus rau TLC phaj.
Yuav ua li cas koj nqa tawm nyias txheej chromatography?
Siv nyias txheej chromatography txhawm rau txheeb xyuas cov tebchaw Ib qho me me ntawm qhov sib xyaw yog muab tso rau ntawm lub hauv paus kab ntawm lub nyias txheej phaj, thiab zoo sib xws me me ntawm cov amino acids paub yog muab tso rau ib sab ntawm nws. Lub phaj yog tom qab ntawd sawv ntsug hauv cov kuab tshuaj uas tsim nyog thiab sab laug kom txhim kho zoo li ua ntej.
Pom zoo:
Puas yog glycerol xav tau daim nyias nyias nyias kom hla daim nyias nyias?
Glycerol yog lipid soluble yog li nws diffuses los ntawm yooj yim diffusion ncaj qha los ntawm lub cell membrane thaum cov piam thaj yog ib tug polar molecule yog li nws diffuses los ntawm kev yooj yim diffusion uas txhais tau hais tias nws xav tau ib tug channel protein ua hauj lwm thiab qhov no txhais tau tias thaj chaw saum npoo rau cov piam thaj kom nkag mus yog tsawg dua. tshaj ib qho rau glycerol
Cov txheej nyias nyias chromatography yog dab tsi thiab nws ua haujlwm li cas?
Nyias-txheej chromatography (TLC) yog ib qho txheej txheem chromatography siv los cais cov khoom sib xyaw uas tsis yog-volatile. Tom qab cov qauv tau siv rau ntawm lub phaj, cov kuab tshuaj los yog cov kuab tshuaj sib tov (lub npe hu ua lub sijhawm txawb) yog kos rau ntawm lub phaj ntawm capillary nkaus
Dab tsi yog qhov chaw nyob ruaj khov nyob rau hauv nyias txheej chromatography?
Lub silica gel (los yog alumina) yog qhov chaw nres tsheb. Cov theem nyob ruaj khov rau nyias txheej txheej chromatography kuj feem ntau muaj cov khoom siv fluorescesin UV teeb - vim li cas koj yuav pom tom qab. Lub mobilephase yog cov kuab tshuaj uas tsim nyog los yog sib tov ntawm cov kuab tshuaj
Dab tsi yog lub sijhawm txawb hauv nyias txheej chromatography?
Dab tsi yog lub sijhawm txawb hauv nyias txheej chromatography? Cov theem ntawm lub xov tooj txawb yog cov kuab tshuaj uas tsim nyog los yog sib xyaw ntawm cov kuab tshuaj. Cov theem txawb ntws los ntawm theem nyob ruaj khov thiab nqa cov khoom sib xyaw nrog nws. Cov khoom sib txawv taug kev ntawm tus nqi sib txawv
Lub hauv paus ntsiab lus ntawm nyias txheej chromatography yog dab tsi?
Chromatography ua haujlwm ntawm lub hauv paus ntsiab lus uas sib txawv tebchaw yuav muaj kev sib txawv solubilities thiab adsorption rau ob theem ntawm lawv yuav tsum tau muab faib. Thin Layer Chromatography (TLC) yog cov txheej txheem ua kua uas muaj ob theem yog cov khoom (stationary phase) thiab cov kua (qib txav mus)