Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?
Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?

Video: Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?

Video: Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?
Video: zoo nyob tag npaum no xoom ceev2 kom phev los tso mam saib seb yog leej twg 2024, Kaum ib hlis
Anonim

Rau silica gel-coated TLC Daim hlau, lub zog eluent nce hauv qhov kev txiav txim hauv qab no: perfluoroalkane (tsis muaj zog), hexane, pentane, carbon tetrachloride, benzene / toluene, dichloromethane, diethyl ether, ethyl acetate, acetonitrile, acetone, 2-propanol / n-butanol, dej,, triethylamine, acetic acid, formic acid

Tsuas yog li ntawd, vim li cas thiaj li sib xyaw cov kuab tshuaj siv hauv chromatography?

1 Teb. Cov kuab tshuaj yog siv los pab cais cov Cheebtsam ntawm a sib tov . Cov solute xaiv yuav tsum muaj peev xwm yaj cov khoom ntawm cov sib tov . Ntawm no yog ib daim vis dis aus ntawm kev sim ua los cais cov khoom ntawm cov dej soluble number case.

Ib sab saum toj no, vim li cas ethyl acetate yog cov kuab tshuaj zoo rau TLC? Yog hais tias tus polar attraction ntawm tus qauv thiab silica ntau dua tus qauv yuav nyob ntau dua nyob rau hauv silica es eluting. Yog li ntawd yog hais tias tus kuab tshuaj muaj ntau polarity nws zoo dua. Polarity ntawm ethyl acetate hmoov yog ntau tshaj 4 uas siab tshaj ntau lwm yam kuab tshuaj thiab yog vim li cas ethyl acetate hmoov yog siv.

Tsis tas li ntawd, cov kuab tshuaj cuam tshuam li cas TLC?

Lub zog eluting ntawm cov kuab tshuaj nce nrog polarity. Yog li ntawd, tsis tshua muaj polarity tebchaw yuav eluted nrog tsawg polarity cov kuab tshuaj , thaum cov polarity ntau dua xav tau cov kuab tshuaj ntawm polarity siab dua. Lub zog muaj zog yog khi rau lub adsorbent, qeeb qeeb nws txav mus rau TLC phaj.

Yuav ua li cas koj nqa tawm nyias txheej chromatography?

Siv nyias txheej chromatography txhawm rau txheeb xyuas cov tebchaw Ib qho me me ntawm qhov sib xyaw yog muab tso rau ntawm lub hauv paus kab ntawm lub nyias txheej phaj, thiab zoo sib xws me me ntawm cov amino acids paub yog muab tso rau ib sab ntawm nws. Lub phaj yog tom qab ntawd sawv ntsug hauv cov kuab tshuaj uas tsim nyog thiab sab laug kom txhim kho zoo li ua ntej.

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