Video: Puas yog Northern blotting siv txwv enzymes?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Thawj kauj ruam hauv Northern blotting yuav tsum tau cais ntawm RNA los ntawm cov qauv lom. Thaum RNA raug cais tawm, cov qauv RNA raug cais los ntawm qhov loj ntawm gel electrophoresis. Thawj kauj ruam nyob rau yav qab teb Blotting Yuav ua kom tiav kev zom zaub mov ntawm DNA los soj ntsuam nrog a txwv enzyme.
Ntawm no, Northern blot ua dab tsi?
A qaum teb blot yog ib txoj kev kuaj siv los kuaj xyuas cov RNA molecules ntawm cov sib tov ntawm RNA. Northern blotting tau siv los txheeb xyuas tus qauv ntawm RNA los ntawm ib qho nqaij mos lossis hom cell txhawm rau ntsuas RNA kev qhia ntawm cov noob caj noob ces.
Ib tug kuj yuav nug, dab tsi cov ntaub ntawv yog soj ntsuam los ntawm cov txheej txheem ntawm Northern blotting? RNA
Tib neeg kuj nug, qhov txawv ntawm Southern blotting thiab Northern blotting yog dab tsi?
A Southern blot (sau ntawv nrog a lub peev "S" vim nws muaj npe tom qab British biologist Edwin Qab teb ) feem ntau yog siv rau kev tshawb pom ntawm DNA ib ntus hauv a DNA cov qauv. Cov qaum teb blot yog siv tseem ceeb los kawm txog noob caj noob ces los ntawm kev tshawb pom ntawm RNA lossis RNA hom (xws li mRNA).
Lub hom phiaj ntawm prehybridization yog dab tsi?
Prehybridization (Kev thaiv) Salmon phev DNA feem ntau yog siv los ua tus neeg sawv cev thaiv kev tiv thaiv kev sojntsuam los ntawm kev ua raws li daim nyias nyias, kom ntseeg tau tias nws tsuas yog cuam tshuam nrog cov DNA xav tau uas tau xa mus rau daim nyias nyias.
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Lub ntuj puas xiav vim dej hiav txwv los yog dej hiav txwv xiav vim lub ntuj?
'Lub hiav txwv zoo li xiav vim liab, txiv kab ntxwv thiab daj (ntev wavelength teeb) yog absorbed ntau zog los ntawm dej tshaj yog xiav (luv wavelength light). Yog li thaum lub teeb dawb ntawm lub hnub nkag mus rau hauv dej hiav txwv, nws feem ntau yog xiav uas tau rov qab los. Ib yam li ntawd, lub ntuj xiav.'