Video: Cov txheej nyias nyias chromatography yog dab tsi thiab nws ua haujlwm li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nyias - txheej chromatography ( TLC ) yog a chromatography txheej txheem siv los cais cov tsis-volatile sib tov. Tom qab cov qauv tau siv rau ntawm lub phaj, cov kuab tshuaj los yog cov kuab tshuaj sib tov (lub npe hu ua lub sijhawm txawb) yog kos rau ntawm lub phaj ntawm capillary nkaus.
Xav txog qhov no, qhov txawv ntawm nyias txheej chromatography thiab ntawv chromatography yog dab tsi?
Qhov yooj yim Qhov sib txawv ntawm nyias txheej chromatography ( TLC) thiab ntawv chromatography (PC) yog qhov ntawd, thaum lub sijhawm nyob ruaj khov hauv PC yog ntawv , nyob ruaj khov theem nyob rau hauv TLC yog a nyias txheej ntawm ib qho inert tshuaj txhawb rau ntawm lub tiaj tus, unreactive nto.
Rf nqi yog dab tsi? Cov Rf nqi txhais tau tias yog qhov sib piv ntawm qhov kev ncua deb txav los ntawm cov kua nplaum (xws li cov dye lossis pigment nyob rau hauv kev sim) thiab qhov kev ncua deb txav los ntawm cov kuab tshuaj (lub npe hu ua Solvent pem hauv ntej) raws daim ntawv, qhov twg ob qhov kev ncua deb yog ntsuas los ntawm lub hauv paus chiv keeb los yog Application Baseline, uas yog lub ntsiab lus uas tus qauv yog
Hauv qhov no, puas yog silica polar lossis nonpolar?
Silica gel, feem ntau siv nyob ruaj khov theem, muaj cov mis mos SiO2. Txawm li cas los xij, ntawm qhov chaw ntawm silica gel hais, cov dangling oxygen atoms raug khi rau protons. Lub xub ntiag ntawm cov no pawg hydroxyl renders nto ntawm silica gel heev polar.
Vim li cas tus number case tsis siv hauv chromatography?
Ink yog sib xyaw ntawm ob peb dyes thiab yog li peb tuaj yeem cais cov xim ntawm ib leeg siv chromatography . Thaum twg tus cwj mem raug rau qee cov kuab tshuaj cov xim yaj thiab tuaj yeem cais tawm. txawv tus cwj mem cwjm siv ntau hom tus cwj mem thiab qhov no yog pom tseeb thaum koj nthuav tawm cov tus cwj mem mus rau qhov hnyav.
Pom zoo:
Puas yog glycerol xav tau daim nyias nyias nyias kom hla daim nyias nyias?
Glycerol yog lipid soluble yog li nws diffuses los ntawm yooj yim diffusion ncaj qha los ntawm lub cell membrane thaum cov piam thaj yog ib tug polar molecule yog li nws diffuses los ntawm kev yooj yim diffusion uas txhais tau hais tias nws xav tau ib tug channel protein ua hauj lwm thiab qhov no txhais tau tias thaj chaw saum npoo rau cov piam thaj kom nkag mus yog tsawg dua. tshaj ib qho rau glycerol
Nyias txheej chromatography txawv ntawm daim ntawv chromatography li cas?
Qhov sib txawv ntawm cov txheej nyias nyias chromatography (TLC) thiab ntawv chromatography (PC) yog tias, thaum lub sij hawm nyob ruaj ruaj hauv PC yog ntawv, cov theem nyob ruaj khov hauv TLC yog txheej nyias ntawm cov khoom siv tsis zoo uas txhawb nqa ntawm lub tiaj tus, tsis muaj zog
Dab tsi yog cov haujlwm sib txawv ntawm daim nyias nyias?
Kev ua haujlwm ntawm Membrane Proteins Membrane proteins tuaj yeem ua haujlwm ntau yam haujlwm tseem ceeb: Kev sib tshuam - Ua haujlwm txuas thiab koom nrog ob lub hlwb ua ke. Enzymes - Kho rau daim nyias nyias localises metabolic txoj kev. Tsheb thauj mus los - Lub luag haujlwm rau kev yooj yim diffusion thiab nquag thauj
Dab tsi yog chromatography thiab nws ua haujlwm raws nraim li cas?
Chromatography yog ib txoj hauv kev ntawm kev sib cais ntawm cov tshuaj sib tov, uas yog nyob rau hauv cov roj los yog kua daim ntawv, byletting lawv maj mam dhau los ntawm lwm yam khoom, uas feem ntau yog ib tug kua los yog khoom
Dab tsi yog cov kuab tshuaj siv hauv nyias txheej chromatography?
Rau silica gel-coated TLC daim hlau, lub zog eluent nce hauv qhov kev txiav txim hauv qab no: perfluoroalkane (tsis muaj zog), hexane, pentane, carbon tetrachloride, benzene / toluene, dichloromethane, diethyl ether, ethyl acetate, acetonitrile, acetone / 2-propylene. -butanol, dej, methanol, triethylamine, acetic acid, formic acid