Cov txheej txheem:
Video: Cov txheej txheem Southern blotting yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
A Southern blot yog ib txoj hauv kev siv hauv molecular biology txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau kuaj DNA. Southern blotting ua ke hloov pauv ntawm electrophoresis-sib cais DNA tawg mus rau lub lim membrane thiab tom qab nrhiav kom pom cov tawg los ntawm kev sojntsuam hybridization.
Yog li ntawd, dab tsi yog cov kauj ruam hauv Southern blotting?
Step-by-Step Guide to Southern Blot Analysis
- Kauj Ruam 1 DNA zom.
- Kauj Ruam 2 Gel electrophoresis.
- Kauj Ruam 3 Blotting.
- Kauj Ruam 4 Tshawb xyuas daim ntawv lo.
- Kauj Ruam 5 Hybridization & ntxuav.
- Kauj Ruam 6 Kev Tshawb Fawb.
Tsis tas li ntawd, vim li cas tib neeg ua rau Southern blotting? Southern blotting yog tsim los nrhiav ib qhov sib lawv liag ntawm DNA nyob rau hauv ib tug complex sib tov. Piv txwv li, Southern Blotting tuaj yeem siv los nrhiav cov noob tshwj xeeb hauv tag nrho genome. Tus nqi ntawm DNA xav tau rau cov txheej txheem no yog nyob ntawm qhov loj thiab cov haujlwm tshwj xeeb ntawm kev sojntsuam.
Hais txog qhov no, dab tsi yog blotting txheej txheem?
Blotting cov txheej txheem yog cov kws tshawb fawb siv los cais cov hom molecules. Hauv cov cell, lawv muaj nyob ua ke. Blotting Feem ntau yog ua los ntawm kev cia cov khoom sib xyaw ntawm DNA, RNA lossis cov protein ntws los ntawm cov nplais ntawm gel.
Puas yog Southern blotting tib yam li gel electrophoresis?
A Southern blot yog ib txoj hauv kev sim siv los txheeb xyuas cov DNA tshwj xeeb ntawm cov khoom ntawm ntau lwm DNA molecules. Cov txheej txheem tau muaj npe tom qab nws tus neeg tsim khoom, Edward Qab teb . Qhov sib tov ntawm DNA tawg yog ces sib cais raws li qhov loj me los ntawm txoj kev hu ua gel electrophoresis.
Pom zoo:
Koj nkag siab dab tsi los ntawm Southern blotting txheej txheem?
Ib lub Southern blot yog ib txoj hauv kev siv hauv molecular biology txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau kuaj DNA. Yav qab teb blotting sib txuas cov kev hloov ntawm electrophoresis-sib cais DNA tawg mus rau ib lub lim membrane thiab tom qab fragment nrhiav tau los ntawm kev sojntsuam hybridization
Dab tsi yog cov txheej txheem uas tsim cov ntawv luam tshiab ntawm cov kab mob caj ces?
Cov txheej txheem ntawm DNA replication tsim ib daim qauv tshiab ntawm cov kab mob cov ntaub ntawv caj ces kom dhau mus rau lub cell tshiab. Dawb ntab nucleotides yog txuam nrog lawv cov lus qhuas los ntawm ib qho enzyme hu ua polymerase. Cov no yog 'lub tsev.' Lawv sib sau ua ke DNA tshiab strand nrog txhua tus qub strands
Cov xim dab tsi yog cov hlwb feem ntau ua ntej siv thawj cov xim rau cov txheej txheem Gram stain?
Ua ntej, siv lead ua violet, thawj stain, yog siv rau lub tshav kub-tso smear, muab tag nrho cov hlwb xim liab
Dab tsi yog txheej txheem spontaneous thiab non-spontaneous txheej txheem?
Cov txheej txheem spontaneous yog ib qho uas tshwm sim yam tsis muaj kev cuam tshuam ntawm sab nraud. Ib txoj kev uas tsis yog tshwm sim yuav tsis tshwm sim yog tias tsis muaj kev cuam tshuam ntawm sab nraud
Cov txheej txheem twg yog txheej txheem endothermic?
Cov txheej txheem endothermic yog ib qho txheej txheem uas xav tau lossis nqus lub zog los ntawm nws qhov chaw nyob ib puag ncig, feem ntau yog ua kom sov. Nws yuav yog cov txheej txheem tshuaj, xws li dissolving ammonium nitrate hauv dej, lossis cov txheej txheem ntawm lub cev, xws li melting ntawm cov dej khov nab kuab