Cov txheej txheem:

Cov txheej txheem Southern blotting yog dab tsi?
Cov txheej txheem Southern blotting yog dab tsi?

Video: Cov txheej txheem Southern blotting yog dab tsi?

Video: Cov txheej txheem Southern blotting yog dab tsi?
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A Southern blot yog ib txoj hauv kev siv hauv molecular biology txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau kuaj DNA. Southern blotting ua ke hloov pauv ntawm electrophoresis-sib cais DNA tawg mus rau lub lim membrane thiab tom qab nrhiav kom pom cov tawg los ntawm kev sojntsuam hybridization.

Yog li ntawd, dab tsi yog cov kauj ruam hauv Southern blotting?

Step-by-Step Guide to Southern Blot Analysis

  • Kauj Ruam 1 DNA zom.
  • Kauj Ruam 2 Gel electrophoresis.
  • Kauj Ruam 3 Blotting.
  • Kauj Ruam 4 Tshawb xyuas daim ntawv lo.
  • Kauj Ruam 5 Hybridization & ntxuav.
  • Kauj Ruam 6 Kev Tshawb Fawb.

Tsis tas li ntawd, vim li cas tib neeg ua rau Southern blotting? Southern blotting yog tsim los nrhiav ib qhov sib lawv liag ntawm DNA nyob rau hauv ib tug complex sib tov. Piv txwv li, Southern Blotting tuaj yeem siv los nrhiav cov noob tshwj xeeb hauv tag nrho genome. Tus nqi ntawm DNA xav tau rau cov txheej txheem no yog nyob ntawm qhov loj thiab cov haujlwm tshwj xeeb ntawm kev sojntsuam.

Hais txog qhov no, dab tsi yog blotting txheej txheem?

Blotting cov txheej txheem yog cov kws tshawb fawb siv los cais cov hom molecules. Hauv cov cell, lawv muaj nyob ua ke. Blotting Feem ntau yog ua los ntawm kev cia cov khoom sib xyaw ntawm DNA, RNA lossis cov protein ntws los ntawm cov nplais ntawm gel.

Puas yog Southern blotting tib yam li gel electrophoresis?

A Southern blot yog ib txoj hauv kev sim siv los txheeb xyuas cov DNA tshwj xeeb ntawm cov khoom ntawm ntau lwm DNA molecules. Cov txheej txheem tau muaj npe tom qab nws tus neeg tsim khoom, Edward Qab teb . Qhov sib tov ntawm DNA tawg yog ces sib cais raws li qhov loj me los ntawm txoj kev hu ua gel electrophoresis.

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