Video: Lub tsev qiv ntawv genomic DNA yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
A genomic tsev qiv ntawv yog ib phau ntawm tag nrho genomic DNA los ntawm ib lub cev. Cov DNA yog khaws cia rau hauv cov pej xeem ntawm cov vectors zoo tib yam, txhua qhov muaj qhov sib txawv ntawm qhov sib txawv DNA . Cov fragments ces muab tso rau hauv vector siv DNA ligase.
Raws li txoj cai, lub tsev qiv ntawv genomic tsim tawm li cas?
A genomic DNA tsev qiv ntawv yog ib qho kev sau ntawm DNA tawg uas ua rau tag nrho-ntev genome ntawm lub cev. A genomic tsev qiv ntawv yog tsim los ntawm kev cais DNA ntawm cov hlwb thiab tom qab ntawd nthuav dav nws siv DNA cloning thev naus laus zis.
Tsis tas li ntawd, qhov txawv ntawm lub tsev qiv ntawv genomic thiab lub tsev qiv ntawv cDNA yog dab tsi? Main txawv : genomic DNA muaj introns, cDNA tsis yog. Tab sis koj nrhiav tsis tau cDNA hauv cell (nquag). Kev sib xyaw ntawm plasmid txhais tau hais tias genomic DNA yuav ntev dua.
Lub tsev qiv ntawv DNA muaj dab tsi?
A DNA Library yog ib phau ntawm DNA fragments uas tau cloned rau hauv vectors kom cov kws tshawb fawb tuaj yeem txheeb xyuas thiab cais cov DNA fragments uas txaus siab rau lawv mus kawm ntxiv. Yeej muaj ob hom ntawm tsev qiv ntawv : genomic DNA thiab cDNA tsev qiv ntawv.
Dab tsi yog siv los xaiv cov noob los ntawm cov tsev qiv ntawv genomic?
DNA probes yog stretches ntawm ib leeg-stranded DNA siv txhawm rau txheeb xyuas qhov muaj cov nucleotide sib xyaw ua ke (cov phiaj xwm kab ke) los ntawm hybridization. Lub tsev qiv ntawv Genomic suav nrog coob tus noob nyob rau hauv daim ntawv ntawm txawv nucleotide sequences ntawm DNA fragments thiab lawv tuaj yeem ua tau xaiv nrog kev pab ntawm DNA soj ntsuam.
Pom zoo:
Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?
Txhawm rau tsim lub tsev qiv ntawv genomic, cov kab mob DNA tau muab rho tawm los ntawm cov hlwb thiab tom qab ntawd zom nrog kev txwv enzyme los txiav cov DNA rau hauv cov khoom me me. Tom qab ntawd cov fragments tau muab tso rau hauv vector siv DNA ligase
Lub Tuam Tsev tau saib dab tsi ntawm nws thawj zaug mus rau qhov chaw pub zaub mov thiab lub tsev tua tsiaj?
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Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?
Txhua lub tsev qiv ntawv DNA yog cov khoom sau ntawm DNA tawg uas sawv cev rau ib qho kev lom zem ntawm kev txaus siab. Los ntawm kev tshuaj xyuas DNA los ntawm ib yam kabmob lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam lus nug tseem ceeb. Ob qhov kev siv ntau tshaj plaws rau cov kev sau DNA no yog DNA sequencing thiab noob cloning
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Lub tsev qiv ntawv genomic tsim tau li cas?
Kev tsim kho ntawm lub tsev qiv ntawv genomic suav nrog tsim ntau cov DNA molecules recombinant. Cov kab mob DNA genomic raug muab rho tawm thiab tom qab ntawd zom nrog cov enzyme txwv. Cov vector uas muaj cov khoom sib txuas ntawm genomic DNA tuaj yeem nkag mus rau hauv cov kab mob hauv lub cev