Koj nkag siab dab tsi los ntawm Southern blotting txheej txheem?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

A Southern blot yog ib txoj hauv kev siv hauv molecular biology txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau kuaj DNA. Southern blotting ua ke hloov pauv ntawm electrophoresis-sib cais DNA tawg mus rau lub lim membrane thiab tom qab nrhiav kom pom cov tawg los ntawm kev sojntsuam hybridization.

Raws li txoj cai blotting yog dab tsi?

Blotting cov txheej txheem yog cov kws tshawb fawb siv los cais cov hom molecules. Hauv cov hlwb, lawv muaj nyob ua ke. Blotting Feem ntau yog ua los ntawm kev cia cov khoom sib xyaw ntawm DNA, RNA lossis cov protein ntws los ntawm cov nplais ntawm gel.

Qhov thib ob, Northern thiab Southern blotting yog dab tsi? Northern blot yog ua kom pom ib qho RNA ib ntus. Southern blot yog ua kom paub txog DNA ib ntus. Yeej lawv yog cov txheej txheem tib lub hauv paus ntsiab lus nrog qhov sib txawv me ntsis vim muaj cov hom phiaj sib txawv.

Khaws qhov no hauv kev xav, vim li cas Southern blotting tseem ceeb?

Southern Blotting . Southern blotting yog tsim los nrhiav ib qhov sib lawv liag ntawm DNA nyob rau hauv ib tug complex sib tov. Piv txwv li, Southern Blotting tuaj yeem siv los nrhiav cov noob tshwj xeeb hauv ib qho genome tag nrho. Tus nqi ntawm DNA xav tau rau cov txheej txheem no yog nyob ntawm qhov loj thiab cov haujlwm tshwj xeeb ntawm kev sojntsuam.

Dab tsi yog qhov tseeb ntawm cov xwm txheej hauv Southern blotting?

Muaj plaub kauj ruam tseem ceeb hauv Southern blotting txheej txheem: Hauv thawj kauj ruam, qauv DNA tau tawg los yog zom rau hauv cov khoom me me uas siv cov enzyme txwv. Tom qab digestion, lub DNA fragments raug cais siv gel electrophoresis. Agarose gel feem ntau yog siv rau lub hom phiaj no.