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Video: Koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Enzyme catalysis yog kuaj los ntawm ntsuas qhov tshwm sim ntawm cov khoom lossis ploj ntawm cov reactants. Rau ntsuas ib yam dab tsi, koj yuav tsum muaj peev xwm pom nws. Enzyme kev ntsuam xyuas yog cov kev xeem tsim los rau ntsuas enzyme kev ua si los ntawm ntsuas kev hloov nyob rau hauv concentration ntawm ib tug detectable tshuaj.
Ib yam li ib tug yuav nug, koj ntsuas tus nqi ntawm enzyme ua haujlwm li cas?
Kev ntsuam xyuas enzyme
- Enzyme assay yog txoj hauv kev los ntsuas kev ua haujlwm enzymatic.
- Qhov kom muaj nuj nqis lossis concentration ntawm ib qho enzyme tuaj yeem qhia tau nyob rau hauv cov nyiaj molar, xws li nrog rau lwm yam tshuaj, lossis hais txog kev ua haujlwm hauv enzyme units.
- Kev ua enzyme = moles ntawm substrate hloov dua siab tshiab rau ib chav tsev lub sij hawm = tus nqi × cov tshuaj tiv thaiv ntim.
Ib tug kuj yuav nug, koj ua li cas xam tus nqi ntawm cov tshuaj tiv thaiv? Feem ntau, a cov tshuaj tiv thaiv cuam tshuam qhov kev hloov hauv qhov concentration ntawm ib yam khoom nyob rau lub sijhawm. Koj xam tus tus nqi ntawm cov tshuaj tiv thaiv los ntawm kev faib cov kev hloov hauv concentration los ntawm lub sijhawm dhau mus. Koj tuaj yeem txiav txim siab qhov tus nqi ntawm a tshuaj tiv thaiv graphically, los ntawm kev nrhiav qhov nqes hav ntawm lub concentration nkhaus.
Tsis tas li ntawd, qhov tshuaj tiv thaiv ntawm ib qho enzyme yog dab tsi?
Los ntawm kev nce cov enzyme concentration, qhov siab tshaj plaws cov tshuaj tiv thaiv nce loj heev. Cov lus xaus: Cov tus nqi ntawm ib qho tshuaj tshuaj tiv thaiv nce thaum lub substrate concentration nce. Enzymes tuaj yeem ua kom nrawm heev tus nqi ntawm a tshuaj tiv thaiv . Txawm li cas los xij, cov enzymes ua saturated thaum lub substrate concentration siab.
Dab tsi yog qhov sib npaug dav rau tag nrho cov tshuaj tiv thaiv enzymatic?
Cov Qauv Biochemistry / Enzyme /Michaelis thiab Menten Kev sib npaug . Michaelis-Menten kev sib npaug tshwm sim los ntawm kev sib npaug rau ib cov tshuaj tiv thaiv enzymatic : E + S ↔ ES ↔ E + P, qhov twg E yog tus enzyme , S yog lub substrate, ES yog tus enzyme -substrate complex, thiab P yog cov khoom.
Pom zoo:
Vim li cas cov tshuaj tiv thaiv kev zom zaub mov hu ua hydrolysis cov tshuaj tiv thaiv?
Thaum lub sij hawm zom mov, piv txwv li, decompositionreactions zom cov zaub mov loj rau hauv cov me me los ntawm kev ntxiv cov dej molecules. Hom tshuaj no hu ua hydrolysis. Raws li dej absorbs heatenergy, qee lub zog yog siv los rhuav tshem hydrogenbonds
Yam dab tsi tuaj yeem hloov pauv yog tias koj xav nce tus nqi ntawm cov tshuaj tiv thaiv tshuaj?
Cov yam ntxwv uas cuam tshuam rau cov tshuaj tiv thaiv yog: thaj tsam ntawm cov khoom reactant. concentration los yog siab ntawm ib tug reactant. kub. xwm ntawm cov reactants. muaj/tsis muaj catalyst
Dab tsi tuaj yeem siv los txiav txim siab tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed?
Enzyme catalysis yog kuaj pom los ntawm kev ntsuas qhov pom ntawm cov khoom lossis ploj ntawm cov reactants. Txhawm rau ntsuas qee yam, koj yuav tsum muaj peev xwm pom nws. Enzyme assay yog cov kev ntsuam xyuas tsim los ntsuas kev ua haujlwm ntawm enzyme los ntawm kev ntsuas qhov kev hloov pauv hauv cov ntsiab lus ntawm cov tshuaj uas pom tau
Yuav ua li cas cov khoom tsim tawm ib feeb cuam tshuam rau tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed?
Rau ib qho enzyme catalyzed cov tshuaj tiv thaiv, tus nqi feem ntau yog qhia nyob rau hauv tus nqi ntawm cov khoom tsim tawm ib feeb. Thaum qhov kub thiab txias, qhov cua sov feem ntau nce tus nqi ntawm cov enzyme catalyzed cov tshuaj tiv thaiv vim tias cov reactants muaj zog ntau dua, thiab tuaj yeem ua tiav qhov ua kom muaj zog
Dab tsi yog tshuaj lom neeg cov tshuaj tiv thaiv thiab cov tshuaj tiv thaiv lub cev?
Qhov sib txawv ntawm cov tshuaj tiv thaiv lub cev thiab cov tshuaj tiv thaiv tshuaj yog muaj pes tsawg leeg. Hauv cov tshuaj tiv thaiv tshuaj, muaj kev hloov pauv ntawm cov tshuaj hauv nqe lus nug; nyob rau hauv ib qho kev hloov ntawm lub cev muaj qhov sib txawv ntawm qhov tsos, tsis hnov tsw, los yog cov duab yooj yim ntawm cov qauv ntawm cov teeb meem yam tsis muaj kev hloov pauv hauv cov ntsiab lus