Cov txheej txheem:

Koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed li cas?
Koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed li cas?

Video: Koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed li cas?

Video: Koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed li cas?
Video: Qhia vij lub yeem pov hwm /06/04/2020 2024, Kaum ib hlis
Anonim

Enzyme catalysis yog kuaj los ntawm ntsuas qhov tshwm sim ntawm cov khoom lossis ploj ntawm cov reactants. Rau ntsuas ib yam dab tsi, koj yuav tsum muaj peev xwm pom nws. Enzyme kev ntsuam xyuas yog cov kev xeem tsim los rau ntsuas enzyme kev ua si los ntawm ntsuas kev hloov nyob rau hauv concentration ntawm ib tug detectable tshuaj.

Ib yam li ib tug yuav nug, koj ntsuas tus nqi ntawm enzyme ua haujlwm li cas?

Kev ntsuam xyuas enzyme

  1. Enzyme assay yog txoj hauv kev los ntsuas kev ua haujlwm enzymatic.
  2. Qhov kom muaj nuj nqis lossis concentration ntawm ib qho enzyme tuaj yeem qhia tau nyob rau hauv cov nyiaj molar, xws li nrog rau lwm yam tshuaj, lossis hais txog kev ua haujlwm hauv enzyme units.
  3. Kev ua enzyme = moles ntawm substrate hloov dua siab tshiab rau ib chav tsev lub sij hawm = tus nqi × cov tshuaj tiv thaiv ntim.

Ib tug kuj yuav nug, koj ua li cas xam tus nqi ntawm cov tshuaj tiv thaiv? Feem ntau, a cov tshuaj tiv thaiv cuam tshuam qhov kev hloov hauv qhov concentration ntawm ib yam khoom nyob rau lub sijhawm. Koj xam tus tus nqi ntawm cov tshuaj tiv thaiv los ntawm kev faib cov kev hloov hauv concentration los ntawm lub sijhawm dhau mus. Koj tuaj yeem txiav txim siab qhov tus nqi ntawm a tshuaj tiv thaiv graphically, los ntawm kev nrhiav qhov nqes hav ntawm lub concentration nkhaus.

Tsis tas li ntawd, qhov tshuaj tiv thaiv ntawm ib qho enzyme yog dab tsi?

Los ntawm kev nce cov enzyme concentration, qhov siab tshaj plaws cov tshuaj tiv thaiv nce loj heev. Cov lus xaus: Cov tus nqi ntawm ib qho tshuaj tshuaj tiv thaiv nce thaum lub substrate concentration nce. Enzymes tuaj yeem ua kom nrawm heev tus nqi ntawm a tshuaj tiv thaiv . Txawm li cas los xij, cov enzymes ua saturated thaum lub substrate concentration siab.

Dab tsi yog qhov sib npaug dav rau tag nrho cov tshuaj tiv thaiv enzymatic?

Cov Qauv Biochemistry / Enzyme /Michaelis thiab Menten Kev sib npaug . Michaelis-Menten kev sib npaug tshwm sim los ntawm kev sib npaug rau ib cov tshuaj tiv thaiv enzymatic : E + S ↔ ES ↔ E + P, qhov twg E yog tus enzyme , S yog lub substrate, ES yog tus enzyme -substrate complex, thiab P yog cov khoom.

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