Video: Lub luag haujlwm ntawm 16s rRNA yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Qhov ntev ntawm 16 S rRNA coding gene yog hais txog 1500bp, uas muaj txog 50 lub luag haujlwm ua haujlwm. 16 S rRNA muaj tus lej muaj nuj nqi : ?Lub immobilization ntawm ribosomal proteins ua raws li scaffolding. ?3'end muaj qhov rov qab SD ntu uas siv los khi rau AUG pib codon ntawm mRNA.
Ntawm no, dab tsi yog qhov tseem ceeb ntawm 16s rRNA?
16 S ribosomal RNA ua (los yog 16S rRNA ) yog ib feem ntawm 30S me me subunit ntawm prokaryotic ribosome uas khi rau Shine-Dalgarno ib ntus. Cov gene coding rau nws yog hu ua 16 S rRNA noob thiab siv nyob rau hauv reconstructing phylogenies, vim qhov qeeb ntawm evolution ntawm thaj av no ntawm cov noob.
Tsis tas li ntawd, 16s rRNA gene sequencing yog dab tsi? 16S rRNA gene sequence tsom xam yog ib tug txheej txheem nyob rau hauv cov kab mob taxonomy thiab kev txheeb xyuas, thiab yog raws li nyob rau hauv lub nrhiav kom tau ntawm ib ntus sib txawv (polymorphisms) nyob rau hauv lub hypervariable cheeb tsam ntawm lub 16S rRNA noob uas muaj nyob rau hauv tag nrho cov kab mob.
Ib sab saum toj no, vim li cas 16s rRNA siv rau kev txheeb xyuas kab mob?
Cov 16S ribosomal RNA gene codes rau RNA tivthaiv ntawm 30S subunit ntawm lub kab mob ribosome. Vim yog qhov nyuaj ntawm DNA-DNA hybridization, 16 S rRNA gene sequencing yog siv raws li lub cuab yeej rau txheeb xyuas cov kab mob nyob rau theem hom thiab pab nrog kev sib txawv ntawm kev sib raug zoo kab mob laj [8].
Qhov txawv ntawm 16s rRNA thiab 18s RRNA yog dab tsi?
Lub ntsiab qhov sib txawv ntawm ua kev soj ntsuam nrog 18S rRNA gene cov ntaub ntawv es tsis txhob 16 S rRNA gene data (los yog ITS cov ntaub ntawv) yog cov ntaub ntawv siv los siv rau kev xaiv OTU, cov haujlwm taxonomic, thiab lub tsev tsim qauv, vim nws yuav tsum muaj eukaryotic sequences.
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Qhov txawv ntawm 16s rRNA thiab 18s RRNA yog dab tsi?
Qhov sib txawv tseem ceeb ntawm kev soj ntsuam nrog 18S rRNA gene cov ntaub ntawv es tsis txhob 16S rRNA cov ntaub ntawv noob (lossis cov ntaub ntawv ITS) yog cov ntaub ntawv siv rau OTU xaiv, cov haujlwm taxonomic, thiab cov qauv kev sib koom ua ke, vim nws yuav tsum muaj cov kab ke eukaryotic
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