Video: Qhov txawv ntawm 16s rRNA thiab 18s RRNA yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Lub ntsiab qhov sib txawv ntawm ua kev soj ntsuam nrog 18S rRNA gene cov ntaub ntawv es tsis txhob 16 S rRNA gene data (los yog ITS cov ntaub ntawv) yog cov ntaub ntawv siv los siv rau kev xaiv OTU, cov haujlwm taxonomic, thiab lub tsev tsim qauv, vim nws yuav tsum muaj eukaryotic sequences.
Xav txog qhov no, vim li cas 16s rRNA noob siv rau kev txheeb xyuas?
Cov 16S ribosomal RNA noob cov lej rau RNA tivthaiv ntawm 30S subunit ntawm cov kab mob ribosome. Vim yog qhov nyuaj ntawm DNA-DNA hybridization, 16S rRNA noob sequencing yog siv raws li lub cuab yeej rau txheeb xyuas cov kab mob ntawm hom kab mob thiab pab nrog kev sib txawv ntawm cov kab mob zoo sib xws [8].
Tsis tas li ntawd, eukaryotes puas muaj 16s rRNA? Cov 16 S rRNA Cov noob muaj nyob rau hauv tag nrho cov kab mob, thiab ib daim ntawv muaj feem cuam tshuam tshwm sim nyob rau hauv tag nrho cov hlwb, nrog rau cov ntawm eukaryotes.
Cov lus nug tseem yog, 18s thiab 28s rRNA yog dab tsi?
Cov 28 S / 18S ribosomal RNA Feem ntau siv los ntsuas qhov zoo ntawm tag nrho RNA purified los ntawm ib qho qauv muab. Hauv tib neeg, 28 S rRNA muaj ~ 5070 nucleotides, thiab 18 S muaj 1869 nucleotides, uas muab a 28 S / 18 S piv ~ 2.7. Ib siab 28 S / 18 S piv yog ib qho qhia tau hais tias cov purified RNA tsis zoo thiab tsis tau degraded.
16s rRNA sequencing yog dab tsi?
16 S rRNA noob ib ntus tsom xam yog ib tug txheej txheem nyob rau hauv cov kab mob taxonomy thiab kev txheeb xyuas, thiab yog raws li nyob rau hauv lub nrhiav kom tau ntawm ib ntus sib txawv (polymorphisms) nyob rau hauv lub hypervariable cheeb tsam ntawm lub 16 S rRNA noob uas muaj nyob rau hauv tag nrho cov kab mob.
Pom zoo:
Dab tsi yog qhov txawv ntawm qhov pom tseeb qhov loj thiab qhov ntsuas qhov ntsuas qhov tseeb?
Dab tsi yog qhov txawv ntawm qhov pom tseeb thiab qhov tseeb? Pom meej qhov loj npaum li cas yog qhov ci ntawm lub hnub qub tshwm los ntawm lub ntiaj teb thiab nyob ntawm qhov ci thiab qhov deb ntawm lub hnub qub. Qhov loj npaum li cas yog qhov ci ntawm lub hnub qub yuav tshwm sim los ntawm tus qauv nyob deb
Dab tsi yog qhov txawv ntawm qhov sib txawv ntawm qhov sib txawv thiab qhov sib txawv ntawm qhov ntab?
Integers thiab floats yog ob yam sib txawv ntawm cov ntaub ntawv tus lej. Ib tus lej (feem ntau hu ua anint) yog tus lej tsis muaj tus lej lej. Float isa floating-point number, uas txhais tau hais tias nws yog tus lej uas muaj tus lej lej. Floats siv thaum xav tau ntau dua
Dab tsi yog qhov sib txawv ntawm qhov sib txawv ntawm cov kab sib txuas thiab ntau qhov sib piv?
6. (2 lub cim) Qhov sib txawv ntawm cov kab sib txuas (kev sib piv) thiab ntau qhov sib piv yog dab tsi? Cov kab sib xyaw ua ke yog npaj sib piv; uas yog, tshwj xeeb txhais tau tias yog ua ke nyob rau hauv ntau txoj kev thiab contrasted nrog rau lwm yam kev sib txuas ntawm txhais tau tias
Dab tsi yog qhov txawv ntawm instantaneous thiab nruab nrab ceev dab tsi yog qhov piv txwv loj tshaj ntawm kev ceev ceev?
Qhov nruab nrab ceev yog qhov ceev nruab nrab ntawm lub sijhawm. Instantaneous ceev yuav yog qhov ceev txhua qhov muab tam sim ntawd hauv lub sijhawm ntawd, ntsuas nrog lub ntsuas ntsuas lub sijhawm
Dab tsi yog qhov txawv ntawm microevolution thiab macroevolution Dab tsi yog qee qhov piv txwv ntawm txhua tus?
Microevolution vs. Macroevolution. Piv txwv ntawm cov kev hloov microevolutionary no yuav suav nrog kev hloov ntawm hom xim lossis loj. Macroevolution, piv txwv li, yog siv los hais txog kev hloov pauv ntawm cov kab mob uas tseem ceeb txaus uas, dhau sijhawm, cov kab mob tshiab yuav raug suav tias yog hom tshiab nkaus xwb