Video: Dab tsi yog dynamic quenching?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Quenching hais txog ib qho txheej txheem uas txo cov fluorescence siv ntawm ib qho khoom siv. Ntau yam txheej txheem tuaj yeem ua rau quenching , xws li kev zoo siab hauv lub xeev cov tshuaj tiv thaiv, kev hloov hluav taws xob, kev tsim kho thiab kev sib tsoo quenching . Quenching yog lub hauv paus rau Förster resonance zog hloov pauv (FRET) kev soj ntsuam.
Ua qhov no hauv kev xav, dab tsi yog qhov zoo li qub thiab dynamic quenching?
zoo li qub (tshwm sim vim yog lub xeev hauv av complex ntawm. fluorophore thiab quencher), dynamic (muaj tshwm sim los ntawm diffusion. ntawm quencher rau fluorophorewhile lub tom kawg yog nyob rau hauv nws zoo siab. xeev) los yog yog hais tias ob mechanisms yog tshwm sim.
Ib yam li ntawd, dab tsi ua rau fluorescence quenching? Fluorescence quenching hais txog tej txheej txheem uas txo tus fluorescence kev siv ntawm ib qho qauv. Ntau yam kev sib cuam tshuam molecular tuaj yeem ua rau quenching . Cov no suav nrog kev zoo siab-lub xeev cov tshuaj tiv thaiv, kev hloov pauv molecular, hloov pauv lub zog, kev tsim hauv av-xeev complex, thiab colli-sional. quenching.
Ntawm no, koj tuaj yeem qhia qhov txawv ntawm qhov zoo li qub thiab dynamic quenching?
Cov static quenching mechanism yog tsim los ntawm ib qho intramolecular dimer nruab nrab tus neeg sau xov xwm thiab quencher, los tsim ib qho chaw uas tsis yog-fluorescent hauv av-xeev complex nrog ib qho tshwj xeeb nqus spectrum. Hauv qhov sib piv, FRET quenching mechanism yog dynamic thiab tsis cuam tshuam qhov kev sojntsuam qhov nqus spectrum.
Tus kheej quenching fluorescence yog dab tsi?
Tus kheej - quenching yog hom tshwj xeeb ntawm fluorescence quenching nyob rau hauv uas fluorophore thiab quencher molecules yog tib yam.
Pom zoo:
Dab tsi yog qhov quab yuam ntawm ib qho khoom hauv qhov zoo li qub lossis dynamic equilibrium?
Thaum lub net quab yuam ntawm ib yam khoom sib npaug rau zerothen cov khoom no yog nyob rau ntawm so (staticequilibrium) los yog txav ntawm qhov nrawm nrawm (dynamicequilibrium)
Dab tsi yog qhov txawv ntawm qhov zoo li qub thiab dynamic equilibrium hauv pob ntseg?
Lub pob ntseg tuav ob qho tib si zoo li qub thiab dynamic equilibrium. Static equilibrium yog kev saib xyuas ntawm lub taub hau txoj hauj lwm kom zoo nyob rau hauv cov lus teb rau cov kev hloov hauv linear motion xws li taug kev. Dynamic equilibrium yog kev saib xyuas ntawm txoj hauj lwm lub taub hau kom zoo nyob rau hauv cov lus teb rau rotational zog xws li tig
Dab tsi yog txheej txheem dynamic hauv biology?
Cov lus teb ntawm tes rau kev hloov pauv hauv lawv ib puag ncig, xws li cov zej zog microbial lossis ntau cov kab mob eukaryotic, yog ib qho kev sib tw loj hauv kev tshawb fawb lom neeg. Cov lus teb no suav nrog cov txheej txheem dynamic uas cuam tshuam nrog kev sib txuas, sib dhos, thiab kev hloov pauv ntawm cov tshuab ntawm tes
Koj tuaj yeem qhia qhov txawv ntawm qhov zoo li qub thiab dynamic quenching?
Lub static quenching mechanism yog tsim los ntawm ib qho intramolecular dimer ntawm tus neeg sau xov xwm thiab quencher, los tsim ib qho tsis muaj fluorescent hauv av-xeev complex nrog ib qho tshwj xeeb nqus spectrum. Hauv qhov sib piv, FRET quenching mechanism yog dynamic thiab tsis cuam tshuam rau qhov kev sojntsuam qhov nqus spectrum
Dab tsi yog dynamic yam ntxwv ntawm cov twj paj nruag?
Dynamic yam ntxwv ntawm lub ntsuas ntsuas yog hais txog cov ntaub ntawv uas qhov ntsuas ntsuas tau hloov pauv sai. Rau ib kauj ruam input muaj nuj nqi, lub sij hawm teb tej zaum yuav raug txhais raws li lub sij hawm coj los ntawm lub cuab tam los txiav txim mus rau ib tug teev feem pua ntawm cov kom muaj nuj nqis ntsuas, tom qab daim ntawv thov ntawm cov input