Dab tsi yog dynamic quenching?
Dab tsi yog dynamic quenching?

Video: Dab tsi yog dynamic quenching?

Video: Dab tsi yog dynamic quenching?
Video: Raug koj dag kuv ris siab hlos 3/16/2018 2024, Kaum ib hlis
Anonim

Quenching hais txog ib qho txheej txheem uas txo cov fluorescence siv ntawm ib qho khoom siv. Ntau yam txheej txheem tuaj yeem ua rau quenching , xws li kev zoo siab hauv lub xeev cov tshuaj tiv thaiv, kev hloov hluav taws xob, kev tsim kho thiab kev sib tsoo quenching . Quenching yog lub hauv paus rau Förster resonance zog hloov pauv (FRET) kev soj ntsuam.

Ua qhov no hauv kev xav, dab tsi yog qhov zoo li qub thiab dynamic quenching?

zoo li qub (tshwm sim vim yog lub xeev hauv av complex ntawm. fluorophore thiab quencher), dynamic (muaj tshwm sim los ntawm diffusion. ntawm quencher rau fluorophorewhile lub tom kawg yog nyob rau hauv nws zoo siab. xeev) los yog yog hais tias ob mechanisms yog tshwm sim.

Ib yam li ntawd, dab tsi ua rau fluorescence quenching? Fluorescence quenching hais txog tej txheej txheem uas txo tus fluorescence kev siv ntawm ib qho qauv. Ntau yam kev sib cuam tshuam molecular tuaj yeem ua rau quenching . Cov no suav nrog kev zoo siab-lub xeev cov tshuaj tiv thaiv, kev hloov pauv molecular, hloov pauv lub zog, kev tsim hauv av-xeev complex, thiab colli-sional. quenching.

Ntawm no, koj tuaj yeem qhia qhov txawv ntawm qhov zoo li qub thiab dynamic quenching?

Cov static quenching mechanism yog tsim los ntawm ib qho intramolecular dimer nruab nrab tus neeg sau xov xwm thiab quencher, los tsim ib qho chaw uas tsis yog-fluorescent hauv av-xeev complex nrog ib qho tshwj xeeb nqus spectrum. Hauv qhov sib piv, FRET quenching mechanism yog dynamic thiab tsis cuam tshuam qhov kev sojntsuam qhov nqus spectrum.

Tus kheej quenching fluorescence yog dab tsi?

Tus kheej - quenching yog hom tshwj xeeb ntawm fluorescence quenching nyob rau hauv uas fluorophore thiab quencher molecules yog tib yam.

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