Video: Koj tuaj yeem qhia qhov txawv ntawm qhov zoo li qub thiab dynamic quenching?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov static quenching tshuab yog kev tsim ntawm ib qho intramolecular dimer nruab nrab reporter thiab quencher , los tsim kom tsis muaj fluorescent hauv av-xeev complex nrog a tshwj xeeb absorption spectrum. Hauv qhov sib piv, FRET quenching tshuab yog dynamic thiab tsis cuam tshuam qhov kev sojntsuam qhov nqus spectrum.
Yog li ntawd, dab tsi yog static thiab dynamic quenching?
zoo li qub (tshwm sim vim yog lub xeev hauv av complex ntawm. fluorophore thiab quencher), dynamic (muaj tshwm sim los ntawm diffusion. ntawm quencher rau fluorophorewhile lub tom kawg yog nyob rau hauv nws zoo siab. xeev) los yog yog hais tias ob mechanisms yog tshwm sim.
Dab tsi yog nws tus kheej quenching fluorescence? Tus kheej - quenching yog hom tshwj xeeb ntawm fluorescence quenching nyob rau hauv uas fluorophore thiab quencher molecules yog tib yam.
Tsis tas li ntawd, dab tsi yog static quenching?
Qhov seem zog hloov mechanism yog static quenching (tseem hu ua kev sib cuag quenching ). Tsis zoo li dynamic quenching , static quenching tshwm sim thaum cov molecules tsim ib tug complex nyob rau hauv lub hauv av xeev, i.e. ua ntej excitation tshwm sim.
Dab tsi ua rau fluorescence quenching?
Fluorescence quenching hais txog tej txheej txheem uas txo tus fluorescence kev siv ntawm ib qho qauv. Ntau yam kev sib cuam tshuam molecular tuaj yeem ua rau quenching . Cov no suav nrog kev zoo siab-lub xeev cov tshuaj tiv thaiv, kev hloov pauv molecular, hloov pauv lub zog, kev tsim hauv av-xeev complex, thiab colli-sional. quenching.
Pom zoo:
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