Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?
Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?

Video: Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?

Video: Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?
Video: Niam pog khaws qe 10/19/2017 2024, Hlis ntuj nqeg
Anonim

Tag nrho DNA cov tsev qiv ntawv yog collections ntawm DNA fragments uas sawv cev rau ib qho kev lom zem system ntawm kev txaus siab. Los ntawm kev txheeb xyuas cov DNA los ntawm ib qho kab mob tshwj xeeb lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam tseem ceeb lus nug. Qhov thib ob feem ntau siv rau cov no DNA collections yog DNA sequencing thiab gene cloning.

Tsis tas li ntawd, lub tsev qiv ntawv genomic siv rau dab tsi?

Cov tsev qiv ntawv genomic feem ntau siv rau daim ntawv thov sequencing. Lawv tau ua lub luag haujlwm tseem ceeb hauv tag nrho genome sequencing ntawm ntau yam kab mob, nrog rau tib neeg genome thiab ntau yam kab mob.

Tsis tas li ntawd, qhov twg yog siv los xaiv cov noob los ntawm cov tsev qiv ntawv genomic? DNA probes yog stretches ntawm ib leeg-stranded DNA siv txhawm rau txheeb xyuas qhov muaj cov nucleotide sib xyaw ua ke (cov phiaj xwm kab ke) los ntawm hybridization. Lub tsev qiv ntawv Genomic suav nrog coob tus noob nyob rau hauv daim ntawv ntawm txawv nucleotide sequences ntawm DNA fragments thiab lawv tuaj yeem ua tau xaiv nrog kev pab ntawm DNA soj ntsuam.

Ntawm no, yuav ua li cas lub tsev qiv ntawv genomic tsim?

A genomic DNA tsev qiv ntawv yog ib qho kev sau ntawm DNA tawg uas ua rau tag nrho-ntev genome ntawm lub cev. A genomic tsev qiv ntawv yog tsim los ntawm kev cais DNA ntawm cov hlwb thiab tom qab ntawd nthuav dav nws siv DNA cloning thev naus laus zis.

Dab tsi yog qhov txawv ntawm lub tsev qiv ntawv DNA genomic thiab lub tsev qiv ntawv cDNA?

cDNA Library vs. cDNA Library tsis muaj cov tsis-coding thiab tswj cov ntsiab lus pom hauv genomic DNA . Genomic DNA cov tsev qiv ntawv muab cov ncauj lus kom ntxaws ntxiv txog lub cev, tab sis muaj peev txheej ntau dua los tsim thiab khaws cia.

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