Video: Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Tag nrho DNA cov tsev qiv ntawv yog collections ntawm DNA fragments uas sawv cev rau ib qho kev lom zem system ntawm kev txaus siab. Los ntawm kev txheeb xyuas cov DNA los ntawm ib qho kab mob tshwj xeeb lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam tseem ceeb lus nug. Qhov thib ob feem ntau siv rau cov no DNA collections yog DNA sequencing thiab gene cloning.
Tsis tas li ntawd, lub tsev qiv ntawv genomic siv rau dab tsi?
Cov tsev qiv ntawv genomic feem ntau siv rau daim ntawv thov sequencing. Lawv tau ua lub luag haujlwm tseem ceeb hauv tag nrho genome sequencing ntawm ntau yam kab mob, nrog rau tib neeg genome thiab ntau yam kab mob.
Tsis tas li ntawd, qhov twg yog siv los xaiv cov noob los ntawm cov tsev qiv ntawv genomic? DNA probes yog stretches ntawm ib leeg-stranded DNA siv txhawm rau txheeb xyuas qhov muaj cov nucleotide sib xyaw ua ke (cov phiaj xwm kab ke) los ntawm hybridization. Lub tsev qiv ntawv Genomic suav nrog coob tus noob nyob rau hauv daim ntawv ntawm txawv nucleotide sequences ntawm DNA fragments thiab lawv tuaj yeem ua tau xaiv nrog kev pab ntawm DNA soj ntsuam.
Ntawm no, yuav ua li cas lub tsev qiv ntawv genomic tsim?
A genomic DNA tsev qiv ntawv yog ib qho kev sau ntawm DNA tawg uas ua rau tag nrho-ntev genome ntawm lub cev. A genomic tsev qiv ntawv yog tsim los ntawm kev cais DNA ntawm cov hlwb thiab tom qab ntawd nthuav dav nws siv DNA cloning thev naus laus zis.
Dab tsi yog qhov txawv ntawm lub tsev qiv ntawv DNA genomic thiab lub tsev qiv ntawv cDNA?
cDNA Library vs. cDNA Library tsis muaj cov tsis-coding thiab tswj cov ntsiab lus pom hauv genomic DNA . Genomic DNA cov tsev qiv ntawv muab cov ncauj lus kom ntxaws ntxiv txog lub cev, tab sis muaj peev txheej ntau dua los tsim thiab khaws cia.
Pom zoo:
Lub tsev qiv ntawv genomic DNA yog dab tsi?
Lub tsev qiv ntawv genomic yog ib phau ntawm tag nrho cov genomic DNA los ntawm ib lub cev. DNA yog khaws cia rau hauv cov pej xeem ntawm cov vectors zoo tib yam, txhua qhov muaj qhov sib txawv ntawm DNA. Tom qab ntawd cov fragments tau muab tso rau hauv vector siv DNA ligase
Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?
Txhawm rau tsim lub tsev qiv ntawv genomic, cov kab mob DNA tau muab rho tawm los ntawm cov hlwb thiab tom qab ntawd zom nrog kev txwv enzyme los txiav cov DNA rau hauv cov khoom me me. Tom qab ntawd cov fragments tau muab tso rau hauv vector siv DNA ligase
Vim li cas cov qauv siv computer muaj txiaj ntsig hauv kev tshawb fawb?
Cov khoos phis tawj siv lej, cov ntaub ntawv thiab cov lus qhia hauv computer los tsim cov xwm txheej ntawm lub ntiaj teb tiag. Lawv kuj tuaj yeem kwv yees tias yuav muaj dab tsi tshwm sim - lossis dab tsi tuaj yeem tshwm sim - hauv cov xwm txheej nyuaj, los ntawm cov txheej txheem huab cua mus rau kev nthuav tawm cov lus xaiv thoob plaws hauv lub nroog
Txoj kev faib cov ntaub ntawv twg muab cov ntaub ntawv sib npaug ntawm cov ntaub ntawv lossis cov kev tshuaj xyuas hauv txhua chav kawm cov ntaub ntawv?
Quantile. txhua chav kawm muaj cov yam ntxwv sib npaug. Ib qho kev faib tawm ntau yog zoo haum rau cov ntaub ntawv faib tawm. Quantile muab tib tus lej ntawm cov ntaub ntawv muaj nuj nqis rau txhua chav kawm
Lub tsev qiv ntawv genomic tsim tau li cas?
Kev tsim kho ntawm lub tsev qiv ntawv genomic suav nrog tsim ntau cov DNA molecules recombinant. Cov kab mob DNA genomic raug muab rho tawm thiab tom qab ntawd zom nrog cov enzyme txwv. Cov vector uas muaj cov khoom sib txuas ntawm genomic DNA tuaj yeem nkag mus rau hauv cov kab mob hauv lub cev