Video: Lub tsev qiv ntawv genomic tsim tau li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Kev tsim kho ntawm a genomic tsev qiv ntawv Nws suav nrog tsim ntau cov DNA recombinant molecules. Lub cev genomic DNA raug rho tawm thiab tom qab ntawd zom nrog kev txwv enzyme. Lub vector uas muaj cov khoom seem ntawm genomic DNA tuaj yeem nkag mus rau hauv cov kab mob hauv lub cev.
Raws li txoj cai, genomic thiab cDNA cov tsev qiv ntawv tau tsim li cas?
Genomic thiab cDNA Libraries Genomic libraries yog tsim los ntawm cloning tag nrho genome ntawm lub cev. Ua ntej, ib qho kev sau ntawm txhua hom DNA tawg yog tsim los ntawm kev txwv cov enzymes lossis cov tshuab txiav.
Qhov thib ob, lub tsev qiv ntawv DNA muaj dab tsi? A DNA Library yog ib phau ntawm DNA fragments uas tau cloned rau hauv vectors kom cov kws tshawb fawb tuaj yeem txheeb xyuas thiab cais cov DNA fragments uas txaus siab rau lawv mus kawm ntxiv. Yeej muaj ob hom ntawm tsev qiv ntawv : genomic DNA thiab cDNA tsev qiv ntawv.
Tom qab ntawd, lo lus nug yog, vim li cas cov tsev qiv ntawv genomic thiaj li muaj txiaj ntsig?
Tag nrho DNA cov tsev qiv ntawv yog collections ntawm DNA fragments uas sawv cev rau ib qho kev lom zem system ntawm kev txaus siab. Los ntawm kev txheeb xyuas cov DNA los ntawm ib qho kab mob tshwj xeeb lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam tseem ceeb lus nug. Qhov thib ob feem ntau siv rau cov no DNA collections yog DNA sequencing thiab gene cloning.
Kuv xav tau DNA ntau npaum li cas rau Illumina sequencing?
Yog tias koj tau npaj koj tus kheej Illumina sib haum ua ke cov tsev qiv ntawv, ces peb xav tau yam tsawg kawg 10 nM qauv DNA hauv 10l. Yog tias koj xav tau cov qauv de-multiplexing thov muab peb cov index sequences siv.
Pom zoo:
Lub tsev qiv ntawv genomic DNA yog dab tsi?
Lub tsev qiv ntawv genomic yog ib phau ntawm tag nrho cov genomic DNA los ntawm ib lub cev. DNA yog khaws cia rau hauv cov pej xeem ntawm cov vectors zoo tib yam, txhua qhov muaj qhov sib txawv ntawm DNA. Tom qab ntawd cov fragments tau muab tso rau hauv vector siv DNA ligase
Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?
Txhawm rau tsim lub tsev qiv ntawv genomic, cov kab mob DNA tau muab rho tawm los ntawm cov hlwb thiab tom qab ntawd zom nrog kev txwv enzyme los txiav cov DNA rau hauv cov khoom me me. Tom qab ntawd cov fragments tau muab tso rau hauv vector siv DNA ligase
Lub Tuam Tsev tau saib dab tsi ntawm nws thawj zaug mus rau qhov chaw pub zaub mov thiab lub tsev tua tsiaj?
Lub Tuam Tsev saib 50-52 txhiab tus nyuj nyuj, thiab nws kuj pom tias qee tus nyuj muaj suab nrov dua li lwm tus ntawm nws thawj zaug mus rau qhov chaw pub zaub mov thiab chaw tua tsiaj. Kuv xav tias lub tuam tsev txhais tau hais tias yog nyuj zoo ces kev lag luam yuav zoo thaum nws hais tias 'Kuv ntseeg tias qhov zoo rau nyuj yog qhov zoo rau kev lag luam
Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?
Txhua lub tsev qiv ntawv DNA yog cov khoom sau ntawm DNA tawg uas sawv cev rau ib qho kev lom zem ntawm kev txaus siab. Los ntawm kev tshuaj xyuas DNA los ntawm ib yam kabmob lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam lus nug tseem ceeb. Ob qhov kev siv ntau tshaj plaws rau cov kev sau DNA no yog DNA sequencing thiab noob cloning
Txoj kev faib cov ntaub ntawv twg muab cov ntaub ntawv sib npaug ntawm cov ntaub ntawv lossis cov kev tshuaj xyuas hauv txhua chav kawm cov ntaub ntawv?
Quantile. txhua chav kawm muaj cov yam ntxwv sib npaug. Ib qho kev faib tawm ntau yog zoo haum rau cov ntaub ntawv faib tawm. Quantile muab tib tus lej ntawm cov ntaub ntawv muaj nuj nqis rau txhua chav kawm