Lub tsev qiv ntawv genomic tsim tau li cas?
Lub tsev qiv ntawv genomic tsim tau li cas?

Video: Lub tsev qiv ntawv genomic tsim tau li cas?

Video: Lub tsev qiv ntawv genomic tsim tau li cas?
Video: Niam pog khaws qe 10/19/2017 2024, Kaum ib hlis
Anonim

Kev tsim kho ntawm a genomic tsev qiv ntawv Nws suav nrog tsim ntau cov DNA recombinant molecules. Lub cev genomic DNA raug rho tawm thiab tom qab ntawd zom nrog kev txwv enzyme. Lub vector uas muaj cov khoom seem ntawm genomic DNA tuaj yeem nkag mus rau hauv cov kab mob hauv lub cev.

Raws li txoj cai, genomic thiab cDNA cov tsev qiv ntawv tau tsim li cas?

Genomic thiab cDNA Libraries Genomic libraries yog tsim los ntawm cloning tag nrho genome ntawm lub cev. Ua ntej, ib qho kev sau ntawm txhua hom DNA tawg yog tsim los ntawm kev txwv cov enzymes lossis cov tshuab txiav.

Qhov thib ob, lub tsev qiv ntawv DNA muaj dab tsi? A DNA Library yog ib phau ntawm DNA fragments uas tau cloned rau hauv vectors kom cov kws tshawb fawb tuaj yeem txheeb xyuas thiab cais cov DNA fragments uas txaus siab rau lawv mus kawm ntxiv. Yeej muaj ob hom ntawm tsev qiv ntawv : genomic DNA thiab cDNA tsev qiv ntawv.

Tom qab ntawd, lo lus nug yog, vim li cas cov tsev qiv ntawv genomic thiaj li muaj txiaj ntsig?

Tag nrho DNA cov tsev qiv ntawv yog collections ntawm DNA fragments uas sawv cev rau ib qho kev lom zem system ntawm kev txaus siab. Los ntawm kev txheeb xyuas cov DNA los ntawm ib qho kab mob tshwj xeeb lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam tseem ceeb lus nug. Qhov thib ob feem ntau siv rau cov no DNA collections yog DNA sequencing thiab gene cloning.

Kuv xav tau DNA ntau npaum li cas rau Illumina sequencing?

Yog tias koj tau npaj koj tus kheej Illumina sib haum ua ke cov tsev qiv ntawv, ces peb xav tau yam tsawg kawg 10 nM qauv DNA hauv 10l. Yog tias koj xav tau cov qauv de-multiplexing thov muab peb cov index sequences siv.

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