Video: Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nyob rau hauv thiaj li yuav tsim ib genomic tsev qiv ntawv , lub cev DNA yog muab rho tawm los ntawm cov hlwb thiab ces zom nrog ib tug txwv enzyme txiav lub DNA rau hauv fragments ntawm ib tug tshwj xeeb loj. Cov fragments ces muab tso rau hauv vector siv DNA ligase.
Ua raws li qhov no hauv kev txiav txim siab, kuv puas tuaj yeem tau txais tag nrho kuv cov genome sequenced?
Muaj cov noob rau cov kab mob tsawg yuav tsis ua rau koj muaj tsos mob. Tab sis cov kab mob tshwm sim los ntawm kev ua yuam kev rau ib lub noob caj noob ces - dab tsi geneticists hu ua "pib loj" kev hloov pauv-yog qhov tsawg heev.
Qhov thib ob, nws siv sijhawm ntev npaum li cas los ua kom tiav tag nrho genome? Sequencing technology tau zoo heev nyob rau hauv xyoo tas los no. Sequencing thawj tus tib neeg genome raug nqi txog $1 nphom thiab siv 13 xyoo ua kom tiav; hnub no nws raug nqi li $3,000 txog $5000 thiab siv tsuas yog ib mus rau ob hnub xwb.
Ib yam li ntawd, nws raug nqi npaum li cas los ua kom tag nrho cov genome?
Thawj tag nrho tib neeg genome sequencing nqi kwv yees li $ 2.7 billion hauv 2003. Hauv 2006, lub nqi poob rau $300,000. Nyob rau hauv 2016, lub nqi poob rau $1,000. Dante Labs Tag nrho Genome nqi tsuas yog $699 xwb, hos qee lub lab tseem them $3,000-5,000 rau ib Tag nrho Genome Sequencing.
Lub hom phiaj ntawm lub tsev qiv ntawv DNA yog dab tsi Yuav ua li cas cov noob tshwj xeeb tuaj yeem rov qab los ntawm lub tsev qiv ntawv DNA?
A DNA Library yog ib qho kev sau ua ke ntawm cloned DNA fragments los ntawm ib tug cell, ntaub so ntswg, los yog lub cev. DNA cov tsev qiv ntawv tuaj yeem siv rau cais a tshwj xeeb gene ntawm kev txaus siab, raws li lawv feem ntau suav nrog tsawg kawg ib feem uas muaj cov noob.
Pom zoo:
Lub tsev qiv ntawv genomic DNA yog dab tsi?
Lub tsev qiv ntawv genomic yog ib phau ntawm tag nrho cov genomic DNA los ntawm ib lub cev. DNA yog khaws cia rau hauv cov pej xeem ntawm cov vectors zoo tib yam, txhua qhov muaj qhov sib txawv ntawm DNA. Tom qab ntawd cov fragments tau muab tso rau hauv vector siv DNA ligase
Vim li cas cov tsev qiv ntawv genomic muaj txiaj ntsig?
Txhua lub tsev qiv ntawv DNA yog cov khoom sau ntawm DNA tawg uas sawv cev rau ib qho kev lom zem ntawm kev txaus siab. Los ntawm kev tshuaj xyuas DNA los ntawm ib yam kabmob lossis cov ntaub so ntswg, cov kws tshawb fawb tuaj yeem teb ntau yam lus nug tseem ceeb. Ob qhov kev siv ntau tshaj plaws rau cov kev sau DNA no yog DNA sequencing thiab noob cloning
Lub ntiaj teb yuav hloov li cas yog tias cov nyhuv tsev cog khoom tsis muaj nyob rau hauv tag nrho cov lus nug?
A) Yog tsis muaj cov nyhuv tsev cog khoom, lub ntiaj teb yuav tawg tag nrho nws cov cua sov rau hauv qhov chaw. b) Txhua lub hnub ci hluav taws xob tuaj yeem nqus tau yam tsis muaj lub tsev cog khoom. C) Qhov tshwm sim ntawm tsis muaj cov nyhuv tsev cog khoom yuav yog lub ntiaj teb kub heev uas yeej tsis txias
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Lub tsev qiv ntawv genomic tsim tau li cas?
Kev tsim kho ntawm lub tsev qiv ntawv genomic suav nrog tsim ntau cov DNA molecules recombinant. Cov kab mob DNA genomic raug muab rho tawm thiab tom qab ntawd zom nrog cov enzyme txwv. Cov vector uas muaj cov khoom sib txuas ntawm genomic DNA tuaj yeem nkag mus rau hauv cov kab mob hauv lub cev