Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?
Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?

Video: Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?

Video: Kuv yuav tsim ib lub tsev qiv ntawv genome tag nrho li cas?
Video: Niam pog khaws qe 10/19/2017 2024, Kaum ib hlis
Anonim

Nyob rau hauv thiaj li yuav tsim ib genomic tsev qiv ntawv , lub cev DNA yog muab rho tawm los ntawm cov hlwb thiab ces zom nrog ib tug txwv enzyme txiav lub DNA rau hauv fragments ntawm ib tug tshwj xeeb loj. Cov fragments ces muab tso rau hauv vector siv DNA ligase.

Ua raws li qhov no hauv kev txiav txim siab, kuv puas tuaj yeem tau txais tag nrho kuv cov genome sequenced?

Muaj cov noob rau cov kab mob tsawg yuav tsis ua rau koj muaj tsos mob. Tab sis cov kab mob tshwm sim los ntawm kev ua yuam kev rau ib lub noob caj noob ces - dab tsi geneticists hu ua "pib loj" kev hloov pauv-yog qhov tsawg heev.

Qhov thib ob, nws siv sijhawm ntev npaum li cas los ua kom tiav tag nrho genome? Sequencing technology tau zoo heev nyob rau hauv xyoo tas los no. Sequencing thawj tus tib neeg genome raug nqi txog $1 nphom thiab siv 13 xyoo ua kom tiav; hnub no nws raug nqi li $3,000 txog $5000 thiab siv tsuas yog ib mus rau ob hnub xwb.

Ib yam li ntawd, nws raug nqi npaum li cas los ua kom tag nrho cov genome?

Thawj tag nrho tib neeg genome sequencing nqi kwv yees li $ 2.7 billion hauv 2003. Hauv 2006, lub nqi poob rau $300,000. Nyob rau hauv 2016, lub nqi poob rau $1,000. Dante Labs Tag nrho Genome nqi tsuas yog $699 xwb, hos qee lub lab tseem them $3,000-5,000 rau ib Tag nrho Genome Sequencing.

Lub hom phiaj ntawm lub tsev qiv ntawv DNA yog dab tsi Yuav ua li cas cov noob tshwj xeeb tuaj yeem rov qab los ntawm lub tsev qiv ntawv DNA?

A DNA Library yog ib qho kev sau ua ke ntawm cloned DNA fragments los ntawm ib tug cell, ntaub so ntswg, los yog lub cev. DNA cov tsev qiv ntawv tuaj yeem siv rau cais a tshwj xeeb gene ntawm kev txaus siab, raws li lawv feem ntau suav nrog tsawg kawg ib feem uas muaj cov noob.

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