Video: Dab tsi enzyme HIV siv rov qab transcriptase?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Crystallographic qauv ntawm HIV -1 rov qab transcriptase qhov twg ob lub subunits p51 thiab p66 yog xim thiab cov chaw nquag ntawm polymerase thiab nuclease yog highlighted. A rov qab transcriptase (RT) ib enzyme siv los tsim cov DNA ntxiv (cDNA) los ntawm RNA template, txheej txheem hu ua thim rov qab kev sau ntawv.
Ib yam li ntawd, enzyme reverse transcriptase ua li cas?
Rov qab transcriptase , kuj hu ua RNA-directed DNA polymerase, ib enzyme encoded los ntawm genetic khoom ntawm retroviruses catalyzes kev sau ntawv ntawm retrovirus RNA (ribonucleic acid) rau hauv DNA (deoxyribonucleic acid).
Ib yam li ntawd, HIV puas muaj cov ntaub ntawv thim rov qab? cell-graphics-1a. HIV yog ib tug retrovirus, uas txhais tau hais tias nws nqa ib leeg-stranded RNA yog nws cov khoom siv caj ces es tsis yog ob-stranded DNA tib neeg hlwb. nqa . Retroviruses kuj muaj cov enzymes rov qab transcriptase , uas tso cai rau nws luam RNA rau hauv DNA thiab siv cov DNA "daim ntawv" los kis rau tib neeg, lossis tus tswv, cov hlwb.
Ib tug kuj yuav nug, cov kab mob twg siv rov qab transcriptase?
Cov kab mob kuj siv rov qab transcriptase kom ciaj sia. Cov kab mob hu ua retroviruses muaj ib qho RNA genome thiab convert RNA rov qab mus rau DNA ua ntej nyiag lub cell. Muaj ntau ntau yam kab mob uas siv rov qab transcriptase, xws li Human T-lymphotropic virus (HTVL) hom 1 thiab 2 thiab human immunodeficiency virus (HIV).
HIV siv cov enzymes dab tsi?
Tom qab HIV tau khi rau lub hom phiaj ntawm tes, HIV RNA thiab ntau yam enzymes, suav nrog rov qab transcriptase , integrase, ribonuclease, thiab protease , yog txhaj rau hauv lub cell.
Pom zoo:
Yuav siv txoj hauv kev twg los txiav txim qhov purity ntawm txhua yam ntawm koj cov khoom uas tau rov qab los?
Cov txheej txheem tshuaj yooj yim tshaj plaws suav nrog gravimetry thiab titration. Kuj tseem muaj ntau txoj kev teeb pom kev zoo los yog spectroscopic, xws li UV-VIS spectroscopy, nuclear magnetic resonance thiab infrared spectroscopy. Cov txheej txheem chromatographic, xws li roj chromatography thiab kua chromatography, kuj tseem siv tau
Koj puas rov qab tag nrho cov tshuaj tsis siv rau hauv lawv cov ntim qub?
Tsis txhob muab cov tshuaj rov qab rau hauv lub raj mis reagent; xa rov qab cov tshuaj tsis siv rau hauv lub thawv yuav muaj kev phom sij. Cov khoom siv ntxiv yuav tsum muab tso rau hauv lub thawv pov tseg uas tsim nyog. Thaum twg ua tau, muab cov khoom siv ntau dhau nrog cov neeg nyob ze, tab sis tsis txhob xa rov qab mus rau lub thawv qub
Yuav ua li cas yog tias tus kab mob HIV muaj qhov tsis ua haujlwm rov qab transcriptase enzyme?
Cov enzymes tau encoded thiab siv los ntawm cov kab mob uas siv cov ntaub ntawv rov qab ua ib kauj ruam hauv cov txheej txheem ntawm kev rov ua dua. HIV kis rau tib neeg nrog kev siv cov enzyme no. Yog tsis muaj rov qab transcriptase, tus kab mob genome yuav tsis tuaj yeem koom ua ke rau hauv lub xov tooj ntawm tes, ua rau tsis ua haujlwm rov ua dua
Kev ua haujlwm ntawm HIV 1 thim rov qab transcriptase yog dab tsi?
Tus kab mob HIV-1 thim rov qab transcriptase enzyme yog lub luag haujlwm rau kev luam ib leeg-stranded viral RNA genome rau hauv ob-stranded DNA (Sarafianos et al, 2001). Cov DNA tsim tshiab tuaj yeem muab tso rau hauv tus tswv tsev genome; tus tswv tsev feem ntau yog tib neeg nyob rau hauv rooj plaub ntawm HIV
Puas yog txhua tus kab mob retroviruses siv rov qab transcriptase?
Retroviruses siv thim rov qab transcriptase los hloov lawv ib leeg-stranded RNA rau hauv ob-stranded DNA. Nws yog DNA uas khaws cov genome ntawm tib neeg cov hlwb thiab cov hlwb los ntawm lwm cov ntaub ntawv muaj sia nyob. Thaum hloov los ntawm RNA mus rau DNA, tus kab mob DNA tuaj yeem raug muab tso rau hauv genome ntawm cov kab mob