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Yuav ua li cas ua Acetocarmine?
Yuav ua li cas ua Acetocarmine?

Video: Yuav ua li cas ua Acetocarmine?

Video: Yuav ua li cas ua Acetocarmine?
Video: Tupao Xiong & Nhia Chang - Kuv Tsis Paub Yuav Ua Li Cas (Original) 2024, Kaum ib hlis
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Acetocarmine npaj (1% tov)

Dissolve 10 g carmine (Fisher C579-25) hauv 1 L ntawm 45% glacial acetic acid, ntxiv boileezers, thiab reflux rau 24 teev. Lim rau hauv cov fwj tsaus thiab khaws cia ntawm 4 ° C. Cov tshuaj no tuaj yeem khaws cia rau lub sijhawm ntev.

Dhau li ntawm no, koj yuav ua li cas thiaj ua tau Acetocarmine?

Tsis yog hlau version

  1. Tshav kub ntawm 45% acetic acid (45ml glacial acetic acid / 55ml ntawm distilled dej) rau boil.
  2. Ntxiv 0.5g ntawm Carmine thiab txuas ntxiv cua sov rau 15-20 feeb thaum stirring.
  3. Txias resulting tov.
  4. Lim kom tshem tawm cov dej nag.

Tom qab ntawd, lo lus nug yog, Acetocarmine yog dab tsi? Kev txhais ntawm acetocarmine .: saturated tov ntawm carmine nyob rau hauv 45 feem pua acetic acid siv tshwj xeeb tshaj yog rau cov sai staining ntawm tshiab unfixed chromosomes.

Yog li ntawd, vim li cas Acetocarmine siv hauv mitosis?

Stains yog siv hauv kev tshawb fawb microscopic txhawm rau txhim kho qhov sib txawv ntawm cov khoom siv lom neeg tshwj xeeb hauv cov qauv. Acetocarmine yog li stain siv kom stain nucleic acid hauv cov hlwb. Raws li acetocarmine tshwj xeeb-stain chromosomes sib nrug ntawm cytoplasm, nws tuaj yeem ua tau siv kom pom cov chromosomes hauv mitotic kev kawm.

Vim li cas lub nucleus tsuas yog siv Acetocarmine stain thaum lub sij hawm nuclear staining?

Acetocarmine muab xim liab rau chromosomes thaum nws yog staining . Los ntawm kev siv qhov no stain peb tuaj yeem soj ntsuam ntau theem ntawm chromosomes thaum cell faib. Xaiv xaiv stain DNA, Fuelgen cov tshuaj tiv thaiv yog siv nyob rau hauv kev tswj xyuas.

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