Vim li cas Acetocarmine siv hauv mitosis?
Vim li cas Acetocarmine siv hauv mitosis?

Video: Vim li cas Acetocarmine siv hauv mitosis?

Video: Vim li cas Acetocarmine siv hauv mitosis?
Video: Nkauj mos ab ib txhia tub sab 7/25/2018 2024, Kaum ib hlis
Anonim

acetocarmine yog siv raws li ib tug stain thiaj li ua rau cov hlwb nyob rau hauv mob mitosis kom pom tseeb thiab ze ze rau kev soj ntsuam yooj yim thiab kawm ntawm cell mob mitosis . Txhawm rau stain chromosomes kom lawv pom tau yooj yim thiab peb tuaj yeem pom lawv morphology, qauv thiab tau suav lawv tus lej dhau ntawm metaphase thiab anaphase.

Cov lus nug tseem yog, kev siv Acetocarmine yog dab tsi?

Stains yog siv hauv kev tshawb fawb microscopic txhawm rau txhim kho qhov sib txawv ntawm cov khoom siv lom neeg tshwj xeeb hauv cov qauv. Acetocarmine yog li stain siv kom stain nucleic acid hauv cov hlwb. Raws li acetocarmine tshwj xeeb-stain chromosomes sib nrug ntawm cytoplasm, nws tuaj yeem ua tau siv kom pom cov chromosomes hauv kev tshawb fawb mitotic.

Yuav ua li cas koj ua acetocarmine? Kev npaj acetocarmine (1%) Dissolve 10 g carmine (Fisher C579-25) hauv 1 L ntawm 45% glacial acetic acid, ntxiv boileezers, thiab reflux rau 24 teev. Lim rau hauv cov fwj tsaus thiab khaws cia ntawm 4 ° C. Cov tshuaj no tuaj yeem khaws cia rau lub sijhawm ntev.

Kuj tseem yuav paub yog, vim li cas Acetocarmine siv rau hauv staining ntawm nucleic acid?

Hauv cytochemistry, yooj yim stains nyiam acetocarmines yog siv rau stain yooj yim molecules, xws li nucleus thiab chromosomes. Acetocarmine muab cov xim liab rau chromosomes thaum lub sij hawm nws staining . Xaiv xaiv stain DNA , Fuelgen cov tshuaj tiv thaiv yog siv nyob rau hauv kev tswj xyuas.

Aceto Orcein yog dab tsi?

Kev txhais ntawm aceto ua - orcein .: ib qho tshuaj lom neeg muaj xws li orcein Hauv kev daws nrog acetic acid Cov lus qhia hauv paus tau txais thiab stained nrog aceto ua - orcein , thiab cov hauv paus hniav squashes tau ua.-

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