Cov txheej txheem:
Video: Yuav ua li cas thauj gel electrophoresis?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Chaw thau khoom kuaj thiab khiav ib Agarose Gel:
- Ntxiv thauj khoom tsis yog rau txhua qhov ntawm koj cov qauv DNA.
- Thaum solidified, tso lub agarose gel rau hauv gel lub thawv ( electrophoresis unit).
- Sau gel lub thawv nrog 1xTAE (los yog TBE) kom txog rau thaum lub gel yog npog.
- Ua tib zoo thauj ib tug molecular hnyav ntaiv mus rau hauv thawj kab ntawm lub gel .
Raws li qhov no, ntau npaum li cas DNA koj yuav tsum thauj khoom rau gel electrophoresis?
Tus nqi ntawm DNA mus load rau qhov dej yog variable. Yam tsawg kawg nkaus ntawm DNA uas tuaj yeem kuaj pom nrog ethidum bromide yog 10 ng. DNA ntau txog li 100 ng ib qhov dej yuav ua rau ib tug ntse, huv si band ntawm ib tug ethidium bromide stained gel.
Tsis tas li ntawd, vim li cas ho tsis siv hauv gel electrophoresis es tsis txhob dej? Cov tsis pub Nws yog tsim nyog los tswj cov pH ntawm DNA daws nyob ze rau nruab nrab theem vim hais tias nws muaj peev xwm ua acidic los ntawm electrolysis. Cov hluav taws xob tam sim no tshwm sim los ntawm cov electrodes tuaj yeem ua rau dej molecules kom dissociate thiab tso H + ions.
Tib neeg kuj nug, vim li cas lub cim siv hauv gel electrophoresis?
Cov tawg me me txav nrawm dua, thiab yog li ntawd ntxiv, dua li cov tawg loj dua thaum lawv nab los ntawm qhov gel . Yog vim li cas ib tug marker siv thaum khiav cov fragments ntawm lub gel ? A tus cim muaj cov DNA seem uas paub qhov loj. Cov cim tau khiav hauv txhua qhov gel rau kev sib piv nrog cov tsis paub tawg paj nyob rau hauv lwm yam gel txoj kab.
Ntau npaum li cas DNA tuaj yeem pom ntawm agarose gel?
Feem ntau agarose gels ua yog tsim los ntawm 0.7% thiab 2%. A 0.7% gel yuav qhia zoo sib cais (kev daws teeb meem) ntawm loj DNA fragments (5-10 kb) thiab 2% gel yuav qhia kev daws teeb meem zoo rau cov khoom me me (0.2-1 kb).
Pom zoo:
Yuav ua li cas koj thauj cov qauv hauv kab chromatography?
Txhawm rau thauj cov kab: Dissolve cov qauv hauv qhov tsawg kawg nkaus ntawm cov kuab tshuaj (5-10 tee). Siv lub pipette los yog syringe nrog ib rab koob tuab, drip tus qauv ncaj qha mus rau sab saum toj ntawm silica. Thaum tag nrho cov qauv tau ntxiv lawm, cia cov kem kom ntws tawm kom cov qib hnyav kov rau saum cov silica
Lub hom phiaj ntawm gel electrophoresis yog dab tsi?
Cov ntsiab lus tseem ceeb: Gel electrophoresis yog cov txheej txheem siv los cais DNA tawg raws li lawv qhov loj me. Cov qauv DNA raug thauj mus rau hauv qhov dej (indentations) ntawm ib qho kawg ntawm ib lub gel, thiab siv hluav taws xob tam sim no los rub lawv los ntawm cov gel. DNA fragments raug them tsis zoo, yog li lawv txav mus rau qhov zoo electrode
Dab tsi yog gel electrophoresis siv los cais DNA molecules quizlet?
Cov gel ua haujlwm zoo li lub sieve, sib cais cov DNA sib txawv raws li lawv qhov loj me, vim tias cov DNA me me tuaj yeem txav mus los ntawm cov gel sai dua cov molecules loj. Cov tshuaj nyob rau hauv cov gel uas DNA kis tau los ntawm khi rau DNA thiab pom nyob rau hauv UV teeb
Dab tsi yog kev tswj zoo thiab kev tswj tsis zoo hauv gel electrophoresis?
Kev tswj qhov zoo thiab qhov tsis zoo yog cov qauv uas tau siv los lees paub qhov siv tau ntawm gel electrophoresis sim. Cov kev tswj tau zoo yog cov qauv uas muaj cov cim paub txog DNA lossis cov protein thiab yuav txav mus rau ib txoj hauv kev tshwj xeeb ntawm cov gel. Kev tswj tsis zoo yog cov qauv uas tsis muaj DNA lossis cov protein
Lub zog rau kev thauj mus los ntawm qhov twg thiab vim li cas thiaj li xav tau lub zog rau kev thauj mus los?
Kev thauj mus los nquag yog txheej txheem uas yuav tsum tau txav cov molecules tawm tsam qhov concentration gradient. Cov txheej txheem yuav tsum muaj zog. Lub zog rau cov txheej txheem tau txais los ntawm kev rhuav tshem cov piam thaj siv oxygen hauv kev ua pa aerobic. ATP yog tsim thaum ua pa thiab tso tawm lub zog rau kev thauj mus los