Video: Dab tsi yog kev tswj zoo thiab kev tswj tsis zoo hauv gel electrophoresis?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Qhov zoo thiab kev tswj tsis zoo yog cov qauv uas siv los lees paub qhov siv tau ntawm cov gel electrophoresis kev sim. Kev tswj zoo yog cov qauv uas muaj cov khoom paub txog DNA lossis cov protein thiab yuav tsiv mus nyob ib txoj hauv kev gel . A kev tswj tsis zoo yog ib qho piv txwv uas tsis muaj DNA lossis protein.
Ib yam li ntawd, koj tuaj yeem nug, vim li cas koj thiaj xav tau kev tswj xyuas qhov zoo thiab tsis zoo thaum khiav lub gel?
A kev tswj zoo tau txais kev kho mob nrog cov lus teb paub, kom qhov no zoo cov lus teb tuaj yeem muab piv rau cov lus teb tsis paub txog kev kho mob. Qhov no yog siv hauv electrophoresis los sib piv cov DNA strands rau DNA Standard. Cov kev tswj tsis zoo yog siv thaum tsis xav tau teb.
Qhov thib ob, dab tsi yog cov tswj hauv gel electrophoresis? Nyob rau hauv txhua txoj kev, feem ntau muaj ob hom tswj siv-zoo tswj thiab tsis zoo tswj . Ob leeg yog qhov tseem ceeb los xyuas seb cov txheej txheem puas ua haujlwm raws li tau npaj tseg kom koj cov txiaj ntsig tau raug. Hais tias koj ua PCR thiab tom qab ntawd khiav gel kom pom cov DNA bands.
Nyob rau hauv txoj kev no, dab tsi yog qhov zoo thiab tsis zoo tswj rau?
A kev tswj tsis zoo yog a tswj pab pawg nyob rau hauv ib qho kev sim uas siv cov kev kho mob uas tsis xav tias yuav tsim tau cov txiaj ntsig. A kev tswj zoo yog a tswj pab pawg nyob rau hauv ib qho kev sim uas siv cov kev kho mob uas paub tias yuav ua rau muaj txiaj ntsig.
Dab tsi yog tam sim no nyob rau hauv kev tswj zoo uas tsis yog nyob rau hauv kev tswj tsis zoo?
Qhov nkaus xwb tam sim no nyob rau hauv qhov zoo tswj cev o tsis nyob hauv kev tswj tsis zoo cev is kev tswj zoo DNA thaum lub sij hawm kev tswj tsis zoo raj tsuas muaj cov dej tsis huv deionized.
Pom zoo:
Dab tsi yog qhov txawv ntawm kev tswj thiab qhov tsis tu ncua hauv kev tshawb fawb?
Qhov sib txawv ntawm qhov tsis tu ncua thiab Tswj Ib qhov hloov pauv tsis tu ncua tsis hloov. Ib qho kev tswj hwm qhov sib txawv ntawm lwm qhov kev hloov pauv, tab sis txhob txwm ua kom tas mus li thoob plaws qhov kev sim kom qhia txog kev sib raug zoo ntawm kev vam thiab kev ywj pheej
Dab tsi yog txoj hauv kev zoo li kev tshawb fawb thiab kev sib raug zoo?
Qhov zoo sib xws ntawm natural science thiab social sciences yog ob qho tib si saib cov xwm txheej tshwj xeeb. Tab sis kev soj ntsuam rau social scientist tuaj yeem muab faib ua kev soj ntsuam, nug cov lus nug, kawm cov ntaub ntawv sau. Tab sis tus kws tshawb fawb ntuj tsis tuaj yeem siv cov kev no
Lub hom phiaj ntawm gel electrophoresis yog dab tsi?
Cov ntsiab lus tseem ceeb: Gel electrophoresis yog cov txheej txheem siv los cais DNA tawg raws li lawv qhov loj me. Cov qauv DNA raug thauj mus rau hauv qhov dej (indentations) ntawm ib qho kawg ntawm ib lub gel, thiab siv hluav taws xob tam sim no los rub lawv los ntawm cov gel. DNA fragments raug them tsis zoo, yog li lawv txav mus rau qhov zoo electrode
Dab tsi yog qhov zoo thiab qhov tsis zoo hauv daim duab?
Cov duab zoo yog cov duab ntawm cov khoom tiag tiag (xws li lub qhov rais ncej). Cov duab tsis zoo yog qhov chaw nyob nruab nrab ntawm cov khoom (xws li qhov chaw nyob hauv lub qhov rais ncej)
Vim li cas tsis zoo thiab tsis zoo yog qhov zoo?
Thaum koj muab qhov tsis zoo los ntawm qhov tsis zoo koj tau txais qhov zoo, vim tias ob lub cim tsis zoo raug muab tso tseg