Lub hom phiaj ntawm gel electrophoresis yog dab tsi?
Lub hom phiaj ntawm gel electrophoresis yog dab tsi?

Video: Lub hom phiaj ntawm gel electrophoresis yog dab tsi?

Video: Lub hom phiaj ntawm gel electrophoresis yog dab tsi?
Video: Xf ibthoj qhia txog lub hom phiaj ntawm kev us neej 2024, Kaum ib hlis
Anonim

Cov ntsiab lus tseem ceeb:

Gel electrophoresis yog cov txheej txheem siv los cais cov DNA tawg raws li lawv qhov loj me. DNA cov qauv raug thauj mus rau hauv qhov dej (indentations) ntawm ib kawg ntawm a gel , thiab ib qho hluav taws xob tam sim no yog siv los rub lawv los ntawm gel . DNA fragments raug them tsis zoo, yog li lawv txav mus rau qhov zoo electrode

Tom qab ntawd, ib tug kuj yuav nug, dab tsi yog qhov tseem ceeb ntawm gel electrophoresis?

Kev piav qhia: gel electrophoresis yog siv rau kev sib cais thiab kev sib cais ntawm dna fragments.it yog cov txheej txheem siv rau kev sib cais ntawm cov khoom sib txawv ionic. Nyob rau hauv hluav taws xob teb, dna fragments yog -ive charged molecules txav mus rau anode raws li lawv molecular loj los ntawm agrose gel.

Ib yam li ntawd, lub hom phiaj ntawm gel electrophoresis tom qab PCR yog dab tsi? Siv gel electrophoresis kom pom cov txiaj ntsig ntawm PCR Cov txiaj ntsig ntawm PCR cov tshuaj tiv thaiv feem ntau pom tau (ua pom) siv gel electrophoresis. Gel electrophoresis yog ib txheej txheem nyob rau hauv uas tawg ntawm DNA yog rub los ntawm ib tug gel matrix los ntawm ib tug hluav taws xob tam sim no, thiab nws cais DNA fragments raws li loj.

Yog li ntawd, lub hom phiaj ntawm electrophoresis yog dab tsi?

Zuag qhia tag nrho, electrophoresis Lub hom phiaj los muab txoj hauv kev zoo ntawm kev tshuaj xyuas cov tshuaj, xws li koj cov ntshav thiab DNA (deoxyribonucleic acid, uas nyuaj rau kev sib cais siv cov txheej txheem ib txwm muaj.

Cov txheej txheem ntawm gel electrophoresis yog dab tsi?

Gel electrophoresis yog ib txoj hauv kev rau kev sib cais thiab kev tsom xam ntawm macromolecules (DNA, RNA thiab cov proteins) thiab lawv cov khoom tawg, raws li lawv qhov loj thiab nqi. Nucleic acid molecules raug cais los ntawm kev siv lub teb hluav taws xob kom txav cov khoom tsis zoo los ntawm cov matrix ntawm agarose lossis lwm yam khoom.

Pom zoo: