Cov txheej txheem:
Video: Yuav ua li cas koj thauj cov qauv hauv kab chromatography?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Txhawm rau thauj cov kab:
- Dissolve lub qauv hauv qhov tsawg kawg nkaus ntawm cov kuab tshuaj (5-10 tee).
- Siv ib tug pipette los yog syringe nrog ib tug tuab koob, drip lub qauv ncaj qha mus rau sab saum toj ntawm silica.
- Ib zaug tag nrho qauv tau ntxiv, tso cai rau kem kom ntws tawm kom cov qib hnyav tau kov rau saum cov silica.
Hais txog qhov no, koj siv silica ntau npaum li cas hauv ib kem?
Qhov ntau zoo yog 100/20 txog 100/10 (5 mus rau 10 mL) ntawm elutant rau 100g silica . Nws tsis yog ib lub tswv yim zoo siv ib qho hnyav sib txawv ntawm no - kev sib cais yuav raug cuam tshuam loj heev.
Qhov thib ob, flash kem chromatography ua haujlwm li cas? Flash Chromatography . Nyias txheej chromatography yog siv los saib xyuas cov tshuaj tiv thaiv, tshawb xyuas cov purity ntawm cov tebchaw thiab ua kom zoo dua cov kuab tshuaj tov rau kab chromatography . Nws yog ib qho micro-technique uas yuav tsum tau muaj tsawg heev ntawm compound.
Ntawm no, yog vim li cas nws tseem ceeb tsis txhob cia lub sij hawm nyob twj ywm qhuav tawm thaum khiav ib kem?
Hauv lwm lo lus, slurry hauv lub kem yuav tsum tsis txhob tso cai rau qhuav tawm . Yog tias qhov no tshwm sim nws tuaj yeem tsim cov kab nrib pleb thiab tsis sib xws hauv cov khoom theem , uas yuav txo cov efficiency ntawm kev sib cais. Cov qauv yuav tsum tau sib cais yog loaded nyob rau hauv cov tshuaj nyob rau hauv ib tug haum hnyav, nyiam dua li concentrated li sai tau.
Yuav ua li cas yog tias cov kab khiav qhuav?
Yog koj cia lub kem khiav qhuav silica yuav pib tawg thiab koj yuav tau txais kev sib cais tsis zoo ntawm koj cov tebchaw. Raws li koj khiav tus kem , tsis txhob cia cov qib ntawm cov kuab tshuaj mus qis dua qib ntawm silica gel lossis koj yuav tau txais cov txiaj ntsig tsis zoo.
Pom zoo:
Yuav ua li cas koj ntim kab rau kab chromatography?
Packing a (silica gel) kem: Siv ib daim hlau los ntxiv ib lub paj rwb rau hauv qab ntawm kem. Clamp lub kem mus rau ib lub nplhaib sawv ntsug thiab ntxiv cov xuab zeb txaus kom sau qhov nkhaus ntawm kab. Muab ib tug pinch clamp rau ntawm lub raj, ces sau rau sab 1/4 mus rau 1/3 tag nrho nrog rau qhov intial eluent
Yuav ua li cas koj sau txoj kab nqes nqes intercept daim ntawv nyob rau hauv tus qauv daim ntawv?
Standard form yog lwm txoj hauv kev los sau daim ntawv nqes hav-intercept (as opposed to y=mx+b). Nws sau li Ax+By=C. Koj tuaj yeem hloov daim ntawv nqes hav-intercept rau cov qauv zoo li no: Y=-3/2x + 3. Tom ntej no, koj cais qhov y-intercept (qhov no nws yog 2) zoo li no: Ntxiv 3/2x rau txhua sab ntawm kab zauv kom tau qhov no: 3/2x + y = 3
Yuav ua li cas muaj pes tsawg tus tooj liab atoms koj yuav tsum tau kab ntawm ib sab los tsim ib kab 1'm ntev?
Los ntawm kev sib piv, lub ntiaj teb cov pej xeem tsuas yog hais txog 7? 109 neeg. Yog tias koj tuaj yeem ua kab 100,000,000 tooj liab atoms ib sab, lawv yuav tsim ib txoj kab ntev li 1 cm
Tus qauv qauv yog dab tsi Qhov txawv ntawm tus qauv qauv thiab tus qauv molecular yog dab tsi?
Cov mis mos molecular siv cov cim tshuaj thiab cov ntawv sau npe los qhia cov naj npawb ntawm cov atoms sib txawv hauv cov molecule lossis compound. Ib qho empirical formula muab qhov yooj yim tshaj plaws, tag nrho tus lej piv ntawm atoms hauv ib qho chaw. Cov qauv qauv qhia txog kev sib koom ua ke ntawm cov atoms hauv cov molecule
Vim li cas cov qauv hauv daim duab 1 homologous cov qauv?
Lub xub ntiag ntawm homologous qauv qhia tau hais tias cov kab mob hloov zuj zus los ntawm cov poj koob yawm txwv. 1. Xa mus rau daim duab 1. Siv cov ntaub ntawv Table 1, Qhia qhov tseeb ntawm lub cev qhia rau txhua yam kab mob uas tau teev tseg