Video: Yuav ua li cas crystal violet stain cells?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Crystal violet khi rau DNA thiab cov proteins hauv hlwb thiab yog li ntawd tuaj yeem siv los txheeb xyuas qhov kev ua raws cai ntawm hlwb . Hauv cov txheej txheem no, cov dye ua haujlwm ua ib qho kev sib cuam tshuam dye uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau tus lej ntawm hlwb nyob rau hauv kab lis kev cai.
Cov lus nug tseem yog, yuav ua li cas siv lead ua violet staining ua haujlwm?
Hauv kev tshawb nrhiav biomedical, siv lead ua violet siv rau stain lub nuclei ntawm adherent cell. Hauv daim ntawv thov no, Crystal violet ua haujlwm raws li intercalating dye thiab tso cai rau kom muaj nuj nqis ntawm DNA uas yog proportional rau tus naj npawb ntawm cov cell. Hauv forensics, siv lead ua violet tau siv los tsim cov ntiv tes.
Dab tsi ntawm cov cell ua crystal violet stain? Cov kab mob Gram zoo stain violet vim muaj cov tuab tuab ntawm peptidoglycan hauv lawv cell phab ntsa, uas khaws cov siv lead ua violet cov no hlwb yog ua qias neeg nrog.
Kuj paub, puas yog crystal violet stain nyob lossis cov hlwb tuag?
Thaum qhov kev xeem, tuag cais tawm hlwb raug ntxuav tawm. Qhov seem txuas hlwb yog ua qias neeg nrog Crystal violet , thiab tom qab ib kauj ruam ntxuav, lub Crystal violet dye yog solubilized thiab ntsuas los ntawm absorbance ntawm 595 nm. Tus nqi ntawm Crystal violet staining nyob rau hauv qhov kev ntsuam xyuas yog ncaj qha proportional rau lub cell biomass.
Vim li cas crystal violet siv hauv Gram staining?
Gram staining yog raws li lub peev xwm ntawm cov kab mob cell phab ntsa kom khaws cov siv lead ua violet zas xim thaum kho cov kuab tshuaj. Cov kab mob cell phab ntsa yog ua qias neeg los ntawm siv lead ua violet . Tom qab ntawd iodine yog ntxiv raws li ib tug mordant los ua tus siv lead ua violet -iodine complex kom cov xim tsis tuaj yeem raug tshem tawm yooj yim.
Pom zoo:
Yuav ua li cas ua carbol Fuchsin stain?
Cov lus qhia rau kev npaj ntawm 1% Carbol fuchsin: Siv cov nyiaj tshuav nyiaj li cas tawm 1 g ntawm Basic fuchsin nyob rau hauv sterile 100 ml hwj. 2. Ntxiv 100 ml ntawm cawv thiab yaj cov zas xim los ntawm muab tso rau hauv ib da dej ntawm 60 ° C. Tsis txhob tso cua sov ncaj qha (Kev daws teeb meem 1)
Crystal violet assay yog dab tsi?
Cov khoom muag. Crystal Violet Assay Kit ab232855 yog siv rau cytoxicity thiab cell viability kev tshawb fawb nrog adherent cell kab lis kev cai. Qhov kev ntsuam xyuas yog nyob ntawm kev tshem tawm ntawm cov kab mob sib txuas los ntawm cov kab lis kev cai ntawm tes thaum lub sijhawm tuag ntawm tes. Thaum lub sij hawm ntsuam xyuas, cov cell tuag detached yog ntxuav tawm
Dab tsi yog qhov molar absorptivity tas li ntawm crystal violet?
Molar hnyav: 407.99 g·mol− 1
Yuav ua li cas ib tug crystal ntawm alum txawv ntawm ib tug crystal ntawm poov tshuaj sulfate?
A) Teb yog: potassium aluminium sulfate yog siv lead ua nrog cubic qauv, potassium aluminium sulfate dodecahydrate (alum) yog hydrate (muaj dej los yog nws cov ntsiab lus)
Puas yog Crystal Violet toxic?
Kev cuam tshuam rau Crystal Violet, Nws Cov Tshuaj Toxic, Genotoxic thiab Carcinogenic cuam tshuam rau ib puag ncig thiab nws degradation thiab detoxification rau Environmental Safety. Nws ua raws li tshuaj lom mitotic, muaj zog carcinogen thiab muaj zog clastogene txhawb cov qog loj hlob hauv qee hom ntses. Yog li, CV yog suav tias yog cov khoom siv biohazard