Yuav ua li cas crystal violet stain cells?
Yuav ua li cas crystal violet stain cells?

Video: Yuav ua li cas crystal violet stain cells?

Video: Yuav ua li cas crystal violet stain cells?
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Crystal violet khi rau DNA thiab cov proteins hauv hlwb thiab yog li ntawd tuaj yeem siv los txheeb xyuas qhov kev ua raws cai ntawm hlwb . Hauv cov txheej txheem no, cov dye ua haujlwm ua ib qho kev sib cuam tshuam dye uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau tus lej ntawm hlwb nyob rau hauv kab lis kev cai.

Cov lus nug tseem yog, yuav ua li cas siv lead ua violet staining ua haujlwm?

Hauv kev tshawb nrhiav biomedical, siv lead ua violet siv rau stain lub nuclei ntawm adherent cell. Hauv daim ntawv thov no, Crystal violet ua haujlwm raws li intercalating dye thiab tso cai rau kom muaj nuj nqis ntawm DNA uas yog proportional rau tus naj npawb ntawm cov cell. Hauv forensics, siv lead ua violet tau siv los tsim cov ntiv tes.

Dab tsi ntawm cov cell ua crystal violet stain? Cov kab mob Gram zoo stain violet vim muaj cov tuab tuab ntawm peptidoglycan hauv lawv cell phab ntsa, uas khaws cov siv lead ua violet cov no hlwb yog ua qias neeg nrog.

Kuj paub, puas yog crystal violet stain nyob lossis cov hlwb tuag?

Thaum qhov kev xeem, tuag cais tawm hlwb raug ntxuav tawm. Qhov seem txuas hlwb yog ua qias neeg nrog Crystal violet , thiab tom qab ib kauj ruam ntxuav, lub Crystal violet dye yog solubilized thiab ntsuas los ntawm absorbance ntawm 595 nm. Tus nqi ntawm Crystal violet staining nyob rau hauv qhov kev ntsuam xyuas yog ncaj qha proportional rau lub cell biomass.

Vim li cas crystal violet siv hauv Gram staining?

Gram staining yog raws li lub peev xwm ntawm cov kab mob cell phab ntsa kom khaws cov siv lead ua violet zas xim thaum kho cov kuab tshuaj. Cov kab mob cell phab ntsa yog ua qias neeg los ntawm siv lead ua violet . Tom qab ntawd iodine yog ntxiv raws li ib tug mordant los ua tus siv lead ua violet -iodine complex kom cov xim tsis tuaj yeem raug tshem tawm yooj yim.

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