Video: Puas yog Crystal Violet toxic?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Kev nphav raug rau Crystal Violet , Nws Tshuaj lom , Genotoxic thiab Carcinogenic cuam tshuam rau ib puag ncig thiab nws degradation thiab detoxification rau Environmental Safety. Nws ua raws li mitotic tshuaj lom , muaj zog carcinogen thiab muaj zog clastogene txhawb cov qog loj hlob hauv qee hom ntses. Yog li, CV yog suav tias yog cov khoom siv biohazard.
Cov lus nug tseem yog, dab tsi yog crystal violet ua los ntawm?
Crystal violet los yog gentian violet (tseem hu ua methyl violet 10B lossis hexamethyl pararosaniline chloride) yog triarylmethane dye siv los ua cov kab mob histological thiab hauv Gram txoj kev faib cov kab mob.
Tsis tas li ntawd, yog Crystal Violet acidic lossis yooj yim? Yog hais tias cov xim ntawm cov dye nyob rau hauv qhov zoo ion, raws li nyob rau hauv cov ntaub ntawv saum toj no, nws yog hu ua a yooj yim dye (piv txwv: methylene xiav, siv lead ua violet , safranin). Yog tias qhov xim xim yog nyob rau hauv qhov tsis zoo them ion, nws yog hu ua an acidic dye (piv txwv li: nigrosin, congo liab).
Ntawm no, yog gentian violet tshuaj lom?
Kuj ceeb tias, tsis muaj mob tshuaj lom Cov kev mob tshwm sim tau tshaj tawm tom qab kev tswj hwm ntawm cov nyiaj ntau gentian violet - kho cov ntshav. Gentian violet yog mutagen, tshuaj lom mitotic, thiab clastogen. Cov teebmeem carcinogenic ntawm gentian violet nyob rau hauv nas tau tshaj tawm tsis ntev los no.
Vim li cas gentian violet raug txwv?
Gentian violet yog ib qho tshuaj tua kab mob siv los kho cov kab mob fungal ntawm daim tawv nqaij (piv txwv li, ringworm, ncaws pob taw). Nws kuj muaj cov tshuaj tua kab mob tsis muaj zog thiab tuaj yeem siv rau ntawm kev txiav me me thiab khawb kom tiv thaiv kab mob. Cov khoom no tau raug rho tawm ntawm Canadian kev ua lag luam vim muaj teeb meem kev nyab xeeb.
Pom zoo:
Crystal violet assay yog dab tsi?
Cov khoom muag. Crystal Violet Assay Kit ab232855 yog siv rau cytoxicity thiab cell viability kev tshawb fawb nrog adherent cell kab lis kev cai. Qhov kev ntsuam xyuas yog nyob ntawm kev tshem tawm ntawm cov kab mob sib txuas los ntawm cov kab lis kev cai ntawm tes thaum lub sijhawm tuag ntawm tes. Thaum lub sij hawm ntsuam xyuas, cov cell tuag detached yog ntxuav tawm
Yuav ua li cas crystal violet stain cells?
Crystal violet khi rau DNA thiab cov proteins hauv cov hlwb thiab zoo li no tuaj yeem siv los txheeb xyuas cov kev tswj hwm ntawm cov hlwb. Hauv cov txheej txheem no, cov xim zas ua haujlwm ua cov xim sib txuas uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau cov cell hauv kab lis kev cai
Dab tsi yog qhov molar absorptivity tas li ntawm crystal violet?
Molar hnyav: 407.99 g·mol− 1
Yuav ua li cas ib tug crystal ntawm alum txawv ntawm ib tug crystal ntawm poov tshuaj sulfate?
A) Teb yog: potassium aluminium sulfate yog siv lead ua nrog cubic qauv, potassium aluminium sulfate dodecahydrate (alum) yog hydrate (muaj dej los yog nws cov ntsiab lus)
Dab tsi yog qhov txawv ntawm wavelength ntawm lub teeb liab thiab lub teeb violet?
Violet lub teeb yog hluav taws xob hluav taws xob hluav taws xob nrog wavelengths ntawm 410 nanometers thiab lub teeb liab muaj awavelength ntawm 680 nanometers. Qhov ntau ntawm wavelengths (400 - 700 nm) ntawm lub teeb pom kev yog nyob nruab nrab ntawm lub electromagnetic spectrum (Fig.1)