Cov txheej txheem:

Yuav ua li cas ua carbol Fuchsin stain?
Yuav ua li cas ua carbol Fuchsin stain?

Video: Yuav ua li cas ua carbol Fuchsin stain?

Video: Yuav ua li cas ua carbol Fuchsin stain?
Video: Top 10 Cooking Oils... The Good, Bad & Toxic! 2024, Kaum ib hlis
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Cov lus qhia rau kev npaj ntawm 1% Carbol fuchsin :

Siv cov nyiaj tshuav digital hnyav tawm 1 g ntawm Basic fuchsin ua nyob rau hauv sterile 100 ml hwj. 2. Ntxiv 100 ml ntawm cawv thiab yaj cov xim los ntawm muab tso rau hauv ib da dej ntawm 60 ° C. Tsis txhob tso cua sov ncaj qha ( Kev daws 1).

Yog li ntawd, hom stain yog carbol Fuchsin?

Carbol fuchsin, carbol-fuchsin, los yog carbolfuchsin, yog sib xyaw ntawm phenol thiab yooj yim fuchsin , siv nyob rau hauv cov kab mob staining cov txheej txheem. Nws yog feem ntau siv nyob rau hauv staining ntawm mycobacteria raws li nws muaj affinity rau cov mycolic acids pom nyob rau hauv lawv cov cell membranes.

Tom qab, lo lus nug yog, yog vim li cas yog steaming carbol Fuchsin ib kauj ruam tsim nyog nyob rau hauv Ziehl Neelsen staining? vim yog cov ntsiab lus lipid siab ntawm cov phab ntsa ntawm tes. Lub phenolic compound carbol fuchsin ua yog siv ua thawj stain vim hais tias nws yog lipid soluble thiab nkag mus rau hauv lub cell phab ntsa. Kev pleev xim los ntawm carbol fuchsin ua yog ntxiv txhim kho los ntawm chav cua sov qhov kev npaj kom yaj lub wax thiab cia lub stain txav mus rau hauv lub cell.

Ib tug kuj nug, ua li cas koj ua kua qaub ceev stain?

Cov txheej txheem ntawm Acid-Fast Stain

  1. Npaj cov kab mob smear rau ntawm qhov huv thiab tsis muaj roj swb, siv cov txheej txheem tsis muaj menyuam.
  2. Cia smear rau cua qhuav thiab tom qab ntawd kho cua sov.
  3. Npog lub smear nrog carbol fuchsin stain.
  4. Tshav kub kom txog thaum vapor cia li pib nce (piv txwv li txog 60 C).
  5. Ntxuav cov stain nrog dej huv.

Lub hom phiaj ntawm acid ceev stain yog dab tsi?

Cov kua qaub - ceev stain yog qhov txawv stain siv los txheeb xyuas kua qaub - ceev Cov kab mob xws li cov tswv cuab ntawm genus Mycobacterium. Acid - ceev Cov kab mob muaj peev xwm tiv taus cov tshuaj tua kab mob thiab cov kab mob qhuav. Vim tias cov phab ntsa ntawm tes yog resistant rau feem ntau cov tebchaw, kua qaub - ceev kab mob yuav tsum tau tshwj xeeb staining txheej txheem.

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