Cov txheej txheem:
Video: Yuav ua li cas ua carbol Fuchsin stain?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov lus qhia rau kev npaj ntawm 1% Carbol fuchsin :
Siv cov nyiaj tshuav digital hnyav tawm 1 g ntawm Basic fuchsin ua nyob rau hauv sterile 100 ml hwj. 2. Ntxiv 100 ml ntawm cawv thiab yaj cov xim los ntawm muab tso rau hauv ib da dej ntawm 60 ° C. Tsis txhob tso cua sov ncaj qha ( Kev daws 1).
Yog li ntawd, hom stain yog carbol Fuchsin?
Carbol fuchsin, carbol-fuchsin, los yog carbolfuchsin, yog sib xyaw ntawm phenol thiab yooj yim fuchsin , siv nyob rau hauv cov kab mob staining cov txheej txheem. Nws yog feem ntau siv nyob rau hauv staining ntawm mycobacteria raws li nws muaj affinity rau cov mycolic acids pom nyob rau hauv lawv cov cell membranes.
Tom qab, lo lus nug yog, yog vim li cas yog steaming carbol Fuchsin ib kauj ruam tsim nyog nyob rau hauv Ziehl Neelsen staining? vim yog cov ntsiab lus lipid siab ntawm cov phab ntsa ntawm tes. Lub phenolic compound carbol fuchsin ua yog siv ua thawj stain vim hais tias nws yog lipid soluble thiab nkag mus rau hauv lub cell phab ntsa. Kev pleev xim los ntawm carbol fuchsin ua yog ntxiv txhim kho los ntawm chav cua sov qhov kev npaj kom yaj lub wax thiab cia lub stain txav mus rau hauv lub cell.
Ib tug kuj nug, ua li cas koj ua kua qaub ceev stain?
Cov txheej txheem ntawm Acid-Fast Stain
- Npaj cov kab mob smear rau ntawm qhov huv thiab tsis muaj roj swb, siv cov txheej txheem tsis muaj menyuam.
- Cia smear rau cua qhuav thiab tom qab ntawd kho cua sov.
- Npog lub smear nrog carbol fuchsin stain.
- Tshav kub kom txog thaum vapor cia li pib nce (piv txwv li txog 60 C).
- Ntxuav cov stain nrog dej huv.
Lub hom phiaj ntawm acid ceev stain yog dab tsi?
Cov kua qaub - ceev stain yog qhov txawv stain siv los txheeb xyuas kua qaub - ceev Cov kab mob xws li cov tswv cuab ntawm genus Mycobacterium. Acid - ceev Cov kab mob muaj peev xwm tiv taus cov tshuaj tua kab mob thiab cov kab mob qhuav. Vim tias cov phab ntsa ntawm tes yog resistant rau feem ntau cov tebchaw, kua qaub - ceev kab mob yuav tsum tau tshwj xeeb staining txheej txheem.
Pom zoo:
Yuav ua li cas crystal violet stain cells?
Crystal violet khi rau DNA thiab cov proteins hauv cov hlwb thiab zoo li no tuaj yeem siv los txheeb xyuas cov kev tswj hwm ntawm cov hlwb. Hauv cov txheej txheem no, cov xim zas ua haujlwm ua cov xim sib txuas uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau cov cell hauv kab lis kev cai
Koj npaj li cas van Giison stain?
Txoj Kev 1 Nqa cov seem rau cov dej distilled. 2 Stain nuclei nrog Celestin Blue 5 mins. 3 Yaug hauv dej distilled. 4 Stain hauv haematoxylin 5 mins. 5 Ntxuav kom zoo hauv dej kais dej 5 feeb. 6 Dej nyab nrog Curtis stain 5 mins. 7 Blot. 8 Lub cev qhuav dej sai sai hauv cov cawv, ntshiab thiab mount
Vim li cas peb thiaj cia siab tias cov kab mob Gram tsis zoo yuav stain liab liab thaum lub sij hawm Gram staining txheej txheem?
Thaum cov kab mob gram zoo stain violet vim muaj cov txheej txheem peptidoglycan tuab nyob rau hauv cov phab ntsa ntawm lawv cov cell, cov kab mob gram tsis zoo stain liab, vim yog cov txheej peptidoglycan thinner hauv lawv cov phab ntsa ntawm tes (peptidoglycan txheej tuab dua tso cai rau cov kab mob gram zoo stains violet. tuav lub stain, tab sis ib txheej nyias
Anisaldehyde stain ua haujlwm li cas?
Anisaldehyde - sulfuric acid yog universal reagent rau cov khoom ntuj, uas ua rau muaj xim sib txawv. Nws nyiam stain lub TLC phaj nws tus kheej, thaum cua sov me me, mus rau lub teeb liab xim, thaum lwm pab pawg ua haujlwm zoo li sib txawv ntawm cov xim
Yuav ua li cas ua ib tug Wright stain?
Wright Stain Method Muab 1.0 ml ntawm Wright Stain Solution rau ntawm smear 1 - 3 feeb. Ntxiv 2.0 ml dej distilled los yog Phosphate tsis pH 6.5 thiab cia sawv ntsug ob zaug raws li nyob rau hauv kauj ruam 1. Yaug stained smear nrog dej los yog Phosphate tsis pH 6.5 kom txog rau thaum cov npoo qhia faintly pinkish-liab