Video: Crystal violet assay yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Cov khoom muag. Crystal Violet Assay Cov khoom siv ab232855 yog siv rau kev tshawb fawb cytoxicity thiab cell viability nrog cov kab lis kev cai ntawm tes. Cov soj ntsuam tso siab rau cov detachment ntawm adherent hlwb los ntawm cell kab lis kev cai daim hlau thaum lub sij hawm cell tuag. Thaum lub sij hawm soj ntsuam , tuag detached hlwb raug ntxuav tawm.
Hauv qhov no, siv lead ua violet yog dab tsi?
Crystal violet los yog methyl violet yog siv hauv ntau daim ntawv thov, suav nrog: Raws li qhov ntsuas pH (daj rau violet nrog kev hloov pauv ntawm pH = 1.6) Hauv kev kho mob hauv zej zog, nws yog cov khoom muaj nyob hauv Gram's Stain, siv rau cais cov kab mob. Cov dyes rhuav tshem cov hlwb thiab yog siv li ib nrab-lub zog sab nraud
Ib yam li ntawd, crystal violet stain cells li cas? Crystal violet khi rau DNA thiab cov proteins hauv hlwb thiab yog li ntawd tuaj yeem siv los txheeb xyuas qhov kev ua raws cai ntawm hlwb . Hauv cov txheej txheem no, cov dye ua haujlwm ua ib qho kev sib cuam tshuam dye uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau tus lej ntawm hlwb nyob rau hauv kab lis kev cai.
Ntawm no, Crystal Violet puas txaus ntshai?
Txawm hais tias nws siv ntau, CV tau tshaj tawm tias yog cov tshuaj zas xim rov qab uas nyob hauv ib puag ncig ntev thiab ua rau muaj kev phom sij rau ib puag ncig. Nws ua raws li tshuaj lom mitotic, muaj zog carcinogen thiab muaj zog clastogene txhawb cov qog loj hlob hauv qee hom ntses.
Puas yog Crystal Violet acidic lossis yooj yim?
Yog hais tias cov xim ntawm cov dye nyob rau hauv qhov zoo ion, raws li nyob rau hauv cov ntaub ntawv saum toj no, nws yog hu ua a yooj yim dye (piv txwv: methylene xiav, siv lead ua violet , safranin). Yog tias qhov xim xim nyob rau hauv qhov tsis zoo ion, nws yog hu ua ib acidic dye (piv txwv li: nigrosin, congo liab).
Pom zoo:
Yuav ua li cas crystal violet stain cells?
Crystal violet khi rau DNA thiab cov proteins hauv cov hlwb thiab zoo li no tuaj yeem siv los txheeb xyuas cov kev tswj hwm ntawm cov hlwb. Hauv cov txheej txheem no, cov xim zas ua haujlwm ua cov xim sib txuas uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau cov cell hauv kab lis kev cai
Dab tsi yog qhov molar absorptivity tas li ntawm crystal violet?
Molar hnyav: 407.99 g·mol− 1
Yuav ua li cas ib tug crystal ntawm alum txawv ntawm ib tug crystal ntawm poov tshuaj sulfate?
A) Teb yog: potassium aluminium sulfate yog siv lead ua nrog cubic qauv, potassium aluminium sulfate dodecahydrate (alum) yog hydrate (muaj dej los yog nws cov ntsiab lus)
Dab tsi yog qhov txawv ntawm wavelength ntawm lub teeb liab thiab lub teeb violet?
Violet lub teeb yog hluav taws xob hluav taws xob hluav taws xob nrog wavelengths ntawm 410 nanometers thiab lub teeb liab muaj awavelength ntawm 680 nanometers. Qhov ntau ntawm wavelengths (400 - 700 nm) ntawm lub teeb pom kev yog nyob nruab nrab ntawm lub electromagnetic spectrum (Fig.1)
Puas yog Crystal Violet toxic?
Kev cuam tshuam rau Crystal Violet, Nws Cov Tshuaj Toxic, Genotoxic thiab Carcinogenic cuam tshuam rau ib puag ncig thiab nws degradation thiab detoxification rau Environmental Safety. Nws ua raws li tshuaj lom mitotic, muaj zog carcinogen thiab muaj zog clastogene txhawb cov qog loj hlob hauv qee hom ntses. Yog li, CV yog suav tias yog cov khoom siv biohazard