Crystal violet assay yog dab tsi?
Crystal violet assay yog dab tsi?

Video: Crystal violet assay yog dab tsi?

Video: Crystal violet assay yog dab tsi?
Video: Dab laug raws Dais 7/23/2017 2024, Kaum ib hlis
Anonim

Cov khoom muag. Crystal Violet Assay Cov khoom siv ab232855 yog siv rau kev tshawb fawb cytoxicity thiab cell viability nrog cov kab lis kev cai ntawm tes. Cov soj ntsuam tso siab rau cov detachment ntawm adherent hlwb los ntawm cell kab lis kev cai daim hlau thaum lub sij hawm cell tuag. Thaum lub sij hawm soj ntsuam , tuag detached hlwb raug ntxuav tawm.

Hauv qhov no, siv lead ua violet yog dab tsi?

Crystal violet los yog methyl violet yog siv hauv ntau daim ntawv thov, suav nrog: Raws li qhov ntsuas pH (daj rau violet nrog kev hloov pauv ntawm pH = 1.6) Hauv kev kho mob hauv zej zog, nws yog cov khoom muaj nyob hauv Gram's Stain, siv rau cais cov kab mob. Cov dyes rhuav tshem cov hlwb thiab yog siv li ib nrab-lub zog sab nraud

Ib yam li ntawd, crystal violet stain cells li cas? Crystal violet khi rau DNA thiab cov proteins hauv hlwb thiab yog li ntawd tuaj yeem siv los txheeb xyuas qhov kev ua raws cai ntawm hlwb . Hauv cov txheej txheem no, cov dye ua haujlwm ua ib qho kev sib cuam tshuam dye uas ua rau kom muaj nuj nqis ntawm DNA uas yog ib txwm tuav proportional rau tus lej ntawm hlwb nyob rau hauv kab lis kev cai.

Ntawm no, Crystal Violet puas txaus ntshai?

Txawm hais tias nws siv ntau, CV tau tshaj tawm tias yog cov tshuaj zas xim rov qab uas nyob hauv ib puag ncig ntev thiab ua rau muaj kev phom sij rau ib puag ncig. Nws ua raws li tshuaj lom mitotic, muaj zog carcinogen thiab muaj zog clastogene txhawb cov qog loj hlob hauv qee hom ntses.

Puas yog Crystal Violet acidic lossis yooj yim?

Yog hais tias cov xim ntawm cov dye nyob rau hauv qhov zoo ion, raws li nyob rau hauv cov ntaub ntawv saum toj no, nws yog hu ua a yooj yim dye (piv txwv: methylene xiav, siv lead ua violet , safranin). Yog tias qhov xim xim nyob rau hauv qhov tsis zoo ion, nws yog hu ua ib acidic dye (piv txwv li: nigrosin, congo liab).

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