Video: Lub laser scanning confocal microscope ua haujlwm li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Lub CLSM ua haujlwm hla ib laser beam los ntawm lub teeb qhov chaw aperture uas yog tom qab ntawd tsom los ntawm lub hom phiaj lens rau hauv thaj chaw me me ntawm koj tus qauv thiab cov duab tau tsim los ntawm pixel-los-pixel los ntawm kev sau cov photons tawm ntawm fluorophores hauv cov qauv.
Piv txwv li, confocal microscope ua haujlwm li cas?
Confocal microscopes ua haujlwm ntawm lub hauv paus ntsiab lus ntawm qhov taw tes excitation nyob rau hauv cov qauv (diffraction txwv qhov chaw) thiab taw tes nrhiav pom ntawm qhov tshwm sim fluorescent teeb liab. Ib tug pinhole ntawm lub detector muab lub cev barrier uas blocks tawm-of-focus fluorescence.
Qhov thib ob, qhov loj ntawm lub laser scanning confocal microscope yog dab tsi? Qhov kev ntsuas thawj tiam no dluab corneal lug ntawm × 400 magnification thiab muaj qhov pom ntawm 400 × 400 µm thaum siv nrog lub hom phiaj × 63 lub hom phiaj uas muaj tus lej aperture ntawm 0.9. Nws siv 670 nm liab wavelength Helium-Neon diode laser raws li nws illumination qhov chaw.
Kuj Paub, laser scanning confocal microscopes saib dab tsi?
Confocal laser scanning microscopy (CLSM) tso cai rau kev txiav qhov muag los ntawm cov ntaub so ntswg. Los ntawm kev tshem tawm cov duab tsis pom tseeb, CLSM them nyiaj ntau dua qhov kev daws teeb meem hauv cov ntaub so ntswg nyob thiab tso cai rau pom cov qauv nyob me me li dendritic spines (Fig. 18.7).
Dab tsi yog qhov kev daws teeb meem siab tshaj plaws ntawm laser scanning confocal microscope?
Hauv kev xyaum, lub kev daws teeb meem siab tshaj plaws hauv Z (axial) uas tuaj yeem pom tau hauv a confocal microscope qhov system yog hais txog 0.8µm; 2-3x phem tshaj nyob rau hauv xy-dimension.
Pom zoo:
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