Dab tsi yog confocal microscope siv rau?
Dab tsi yog confocal microscope siv rau?

Video: Dab tsi yog confocal microscope siv rau?

Video: Dab tsi yog confocal microscope siv rau?
Video: Plantae Presents - Sheng-Yang He and Jonathan Jacobs 2024, Kaum ib hlis
Anonim

Confocal microscopy , feem ntau confocal laser scanning microscopy (CLSM) los yog laser confocal luam theej duab microscopy (LCSM), yog cov txheej txheem kho qhov muag kom pom kev daws teeb meem thiab qhov sib piv ntawm micrograph los ntawm kev siv lub spatial pinhole los thaiv lub teeb pom kev hauv cov duab tsim.

Yog li ntawd, vim li cas koj thiaj yuav siv lub tshuab tsom xam confocal?

Feem ntau confocal microscopes siv nyob rau hauv industrial daim ntaub ntawv yog reflection-hom. Lawv muab cov duab daws teeb meem siab nrog rau txhua qhov chaw hauv kev tsom mus rau thoob plaws qhov kev pom, txawm tias cov qauv uas muaj dents thiab protrusions ntawm qhov chaw. Lawv pab kom tsis txhob sib cuag tsis muaj kev puas tsuaj ntawm cov duab peb sab.

Tsis tas li ntawd, qhov magnification ntawm confocal microscope yog dab tsi? Qhov kev ntsuas thawj tiam no dluab corneal lug ntawm × 400 magnification thiab muaj qhov pom ntawm 400 × 400 µm thaum siv nrog lub hom phiaj × 63 lub hom phiaj uas muaj tus lej aperture ntawm 0.9. Nws siv 670 nm liab wavelength Helium-Neon diode laser ua nws qhov chaw teeb pom kev zoo.

Tsis tas li ntawd, qhov txawv ntawm confocal thiab fluorescence microscopy yog dab tsi?

Cov fluorescence microscope tso cai rau kom paub qhov muaj thiab localization ntawm fluorescent molecules hauv qauv. Cov confocal microscope yog ib qho tshwj xeeb fluorescent microscope uas tso cai rau tau txais 3D cov duab ntawm cov qauv nrog kev daws teeb meem zoo. Hauv cov microscopies no, cov qauv muaj fluorescent molecules.

Tus confocal microscope nqi npaum li cas?

Cov nqi ntawm qhov thov confocal microscope yog $ 274, 579 thiab yuav raug sib tw los ntawm lub koom haum cog lus rau ib xyoos twg $ 10, 000 daim ntawv cog lus ua haujlwm, tag nrho nqi ntawm kev hloov pauv yav tom ntej / kev hloov kho tshiab, thiab 80% nyiaj hli txhawb rau tus kws tshaj lij los tswj cov microscope.

Pom zoo: