Video: Dab tsi ua rau fluorescence quenching?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Quenching hais txog tej txheej txheem uas txo tus fluorescence kev siv ntawm ib yam khoom muab. Ntau yam txheej txheem tuaj yeem ua rau quenching , xws li kev zoo siab hauv lub xeev cov tshuaj tiv thaiv, kev hloov hluav taws xob, kev tsim kev sib tsoo thiab kev sib tsoo quenching . Molecular oxygen, iodide ions thiab acrylamide yog cov tshuaj quenchers.
Tsuas yog li ntawd, vim li cas fluorescence quenching tshwm sim?
Fluorescence quenching yog txheej txheem uas txo qhov kev siv ntawm cov fluorescence tso pa tawm. Quenching los ntawm me me molecules nyob rau hauv hnyav los yog khi rau cov protein nyob ze rau fluorophore ua tau txo qis quantum tawm los ntawm cov protein ntau. Quenching muaj tshwm sim los ntawm ntau yam mechanisms.
Dhau li saum toj no, dab tsi yog aggregation ua rau quenching? Abstract. Cov yam ntxwv ntawm zoo-conjugated thiab planar aromatic lug ua π-conjugated luminescent cov ntaub ntawv raug kev txom nyem los ntawm aggregation ua rau quenching (ACQ) cov nyhuv thaum siv cov khoom los yog sib sau ua ke cov xeev, uas cuam tshuam lawv cov ntawv thov hauv optoelectronic li thiab kev siv roj ntsha.
Ua kom pom qhov no, fluorescence quenching ua haujlwm li cas?
Fluorescence quenching yog cov txheej txheem physicochemical uas txo qis qhov kev siv ntawm emission teeb los ntawm fluorescent molecules. Thaum lub molecule absorbs lub teeb, electrons nyob rau hauv nws cov atoms constituent ua zoo siab thiab tau nce mus rau ib tug ntau zog theem.
Yam dab tsi cuam tshuam rau fluorescence?
Peb yam tseem ceeb cuam tshuam rau kev siv zog Cov fluorescence emissions tau soj ntsuam theoretical, suav nrog lub peev xwm nqus ntawm cov photons excitation, fluorescence quantum yield, thiab fluorescence saturation & fluorescence quenching.
Pom zoo:
Dab tsi ntawm pipette yog volumetric thiab nws siv rau dab tsi?
Lub volumetric pipette, noob pipette, lossis lub plab pipette tso cai rau kev ntsuas qhov tseeb (rau plaub qhov tseem ceeb) ntawm qhov ntim ntawm cov tshuaj. Volumetric pipettes feem ntau yog siv rau hauv analytical chemistry los ua cov kev daws teeb meem los ntawm lub hauv paus Tshuag thiab npaj cov kev daws teeb meem rau titration
S sawv rau dab tsi thiab dab tsi tshwm sim hauv theem no?
S theem sawv rau 'Synthesis'. Qhov no yog theem thaum DNA replication tshwm sim. The G2 stage stands for 'GAP 2'
Dab tsi yog cov reagents xav tau rau PCR thiab dab tsi yog qhov ua haujlwm ntawm txhua tus?
Muaj tsib yam tshuaj reagents, lossis cov khoom xyaw, siv hauv PCR: qauv DNA, PCR primers, nucleotides, PCR tsis thiab Taq polymerase. Primers feem ntau yog siv nyob rau hauv khub, thiab DNA ntawm ob lub primers yog amplified thaum lub sij hawm PCR cov tshuaj tiv thaiv
Dab tsi yog dynamic quenching?
Quenching yog hais txog txhua tus txheej txheem uas txo cov fluorescence siv ntawm ib yam khoom. Ntau yam txheej txheem tuaj yeem ua rau quenching, xws li kev zoo siab hauv lub xeev cov tshuaj tiv thaiv, kev hloov hluav taws xob, kev tsim nyuaj thiab kev sib tsoo quenching. Quenching yog lub hauv paus rau Förster resonance zog hloov pauv (FRET) kev soj ntsuam
Dab tsi yog qhov tseem ceeb tshaj plaws uas yuav tsum muaj rau cov kua dej ntws hauv cov kav dej Dab tsi lwm yam cuam tshuam rau cov dej ntws?
Thaum ib qho kev quab yuam sab nraud yog exerted rau ntawm cov kua uas muaj, qhov tshwm sim siab yog kis sib npaug thoob plaws hauv cov kua. Yog li txhawm rau kom dej ntws, dej xav tau qhov sib txawv siab. Cov kav dej kuj tuaj yeem cuam tshuam los ntawm cov kua dej, cov yeeb nkab loj, qhov kub thiab txias (cov kav dej khov), cov kua dej ntom ntom