Cov txheej txheem:
Video: Dab tsi yog cov reagents xav tau rau PCR thiab dab tsi yog qhov ua haujlwm ntawm txhua tus?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Muaj tsib yam yooj yim reagents , los yog cov khoom xyaw, siv nyob rau hauv PCR : DNA template, PCR primers, nucleotides, PCR buffer thiab Taq polymerase. Primers feem ntau yog siv ua khub, thiab DNA ntawm ob lub primers yog amplified thaum lub sij hawm PCR tshuaj tiv thaiv.
Ib sab ntawm qhov no, dab tsi xav tau rau kev tshuaj xyuas PCR?
Basic Cheebtsam ntawm a Cov tshuaj tiv thaiv PCR suav nrog DNA template, primers, nucleotides, DNA polymerase, thiab tsis. Cov qauv DNA feem ntau yog koj cov qauv DNA, uas muaj cov DNA cheeb tsam kom nthuav dav. Primer tsim yog qhov tseem ceeb rau kev ua tiav Cov tshuaj tiv thaiv PCR.
Tsis tas li ntawd, PCR tsis ua haujlwm yog dab tsi? Tsis pub . PCR yog ua nyob rau hauv a tsis pub uas muab cov tshuaj lom neeg tsim nyog rau kev ua haujlwm ntawm DNA polymerase. Cov tsis pub pH feem ntau yog nyob nruab nrab ntawm 8.0 thiab 9.5 thiab feem ntau stabilized los ntawm Tris-HCl. Rau Taq DNA polymerase, ib qho kev tivthaiv nyob rau hauv lub tsis pub yog potassium ion (K+) los ntawm KCl, uas txhawb nqa primer annealing.
Ua raws li qhov xav tau, PCR txoj kev yog dab tsi?
PCR ( polymerase saw cov tshuaj tiv thaiv ) yog a txoj kev txhawm rau txheeb xyuas qhov luv luv ntawm DNA (lossis RNA) txawm nyob rau hauv cov qauv uas tsuas muaj feeb ntau ntawm DNA lossis RNA. PCR yog siv los tsim dua tshiab (ua kom nrov) xaiv cov ntu ntawm DNA lossis RNA. PCR yog qhov ua tau zoo heev hauv qhov tsis paub tseeb ntawm cov ntawv luam tuaj yeem ua los ntawm DNA.
4 kauj ruam ntawm PCR yog dab tsi?
Cov kauj ruam koom nrog hauv Polymerase Chain Reaction hauv DNA Sequence
- Kauj Ruam 1: Denaturation los ntawm Thaum tshav kub kub: Thaum tshav kub kub yog feem ntau ntau tshaj 90 degrees Celsius ntawm cais ob-stranded DNA rau hauv ob qho tib si strands.
- Kauj Ruam 2: Annealing Primer rau Target Sequence:
- Kauj Ruam 3: Extension:
- Kauj Ruam 4: Xaus ntawm Thawj PGR voj voog:
Pom zoo:
Dab tsi yog qhov txawv ntawm qhov sib txawv ntawm qhov sib txawv thiab qhov sib txawv ntawm qhov ntab?
Integers thiab floats yog ob yam sib txawv ntawm cov ntaub ntawv tus lej. Ib tus lej (feem ntau hu ua anint) yog tus lej tsis muaj tus lej lej. Float isa floating-point number, uas txhais tau hais tias nws yog tus lej uas muaj tus lej lej. Floats siv thaum xav tau ntau dua
Lub organelle twg ua haujlwm raws li qhov chaw xa ntawv ntawm lub xov tooj ntawm tes txheeb cov proteins thiab xa lawv mus rau lawv qhov chaw xav tau sab hauv lossis sab nraud ntawm tes?
Golgi Hais txog qhov no, dab tsi organelle yog lub luag haujlwm rau kev thauj mus los? endoplasmic reticulum (ER Qhov thib ob, cov proteins txav mus los ntawm lub cell li cas? Cov proteins txav mus los endomembrane system thiab raug xa tawm los ntawm lub ntsej muag trans ntawm Golgi apparatus nyob rau hauv thauj vesicles uas txav mus los cytoplasm thiab tom qab ntawd fuse nrog plasma membrane tso tawm protein ntau mus rau sab nraum lub cell .
Dab tsi yog qhov ua tau ntawm L rau txhua tus nqi ntawm n?
Subshells. Tus naj npawb ntawm qhov tseem ceeb ntawm tus naj npawb orbitalangular l kuj tuaj yeem siv los txheeb xyuas cov naj npawb ntawm subshells hauv lub plhaub hluav taws xob tseem ceeb: Thaum n = 1, l = 0 (l siv rau ib tus nqi thiab yog li muaj peev xwm tsuas yog ib qho subshell) Thaum n = 2 , l = 0, 1 (ltakes ntawm ob qhov tseem ceeb thiab yog li muaj ob lub subshells)
Tus qauv qauv yog dab tsi Qhov txawv ntawm tus qauv qauv thiab tus qauv molecular yog dab tsi?
Cov mis mos molecular siv cov cim tshuaj thiab cov ntawv sau npe los qhia cov naj npawb ntawm cov atoms sib txawv hauv cov molecule lossis compound. Ib qho empirical formula muab qhov yooj yim tshaj plaws, tag nrho tus lej piv ntawm atoms hauv ib qho chaw. Cov qauv qauv qhia txog kev sib koom ua ke ntawm cov atoms hauv cov molecule
Dab tsi yog qhov txawv ntawm microevolution thiab macroevolution Dab tsi yog qee qhov piv txwv ntawm txhua tus?
Microevolution vs. Macroevolution. Piv txwv ntawm cov kev hloov microevolutionary no yuav suav nrog kev hloov ntawm hom xim lossis loj. Macroevolution, piv txwv li, yog siv los hais txog kev hloov pauv ntawm cov kab mob uas tseem ceeb txaus uas, dhau sijhawm, cov kab mob tshiab yuav raug suav tias yog hom tshiab nkaus xwb