Cov txheej txheem:

Dab tsi yog cov reagents xav tau rau PCR thiab dab tsi yog qhov ua haujlwm ntawm txhua tus?
Dab tsi yog cov reagents xav tau rau PCR thiab dab tsi yog qhov ua haujlwm ntawm txhua tus?

Video: Dab tsi yog cov reagents xav tau rau PCR thiab dab tsi yog qhov ua haujlwm ntawm txhua tus?

Video: Dab tsi yog cov reagents xav tau rau PCR thiab dab tsi yog qhov ua haujlwm ntawm txhua tus?
Video: Yog ntuj tsis pab ntshe kuv tuag lawm 8/6/2018 2024, Lub peb hlis ntuj
Anonim

Muaj tsib yam yooj yim reagents , los yog cov khoom xyaw, siv nyob rau hauv PCR : DNA template, PCR primers, nucleotides, PCR buffer thiab Taq polymerase. Primers feem ntau yog siv ua khub, thiab DNA ntawm ob lub primers yog amplified thaum lub sij hawm PCR tshuaj tiv thaiv.

Ib sab ntawm qhov no, dab tsi xav tau rau kev tshuaj xyuas PCR?

Basic Cheebtsam ntawm a Cov tshuaj tiv thaiv PCR suav nrog DNA template, primers, nucleotides, DNA polymerase, thiab tsis. Cov qauv DNA feem ntau yog koj cov qauv DNA, uas muaj cov DNA cheeb tsam kom nthuav dav. Primer tsim yog qhov tseem ceeb rau kev ua tiav Cov tshuaj tiv thaiv PCR.

Tsis tas li ntawd, PCR tsis ua haujlwm yog dab tsi? Tsis pub . PCR yog ua nyob rau hauv a tsis pub uas muab cov tshuaj lom neeg tsim nyog rau kev ua haujlwm ntawm DNA polymerase. Cov tsis pub pH feem ntau yog nyob nruab nrab ntawm 8.0 thiab 9.5 thiab feem ntau stabilized los ntawm Tris-HCl. Rau Taq DNA polymerase, ib qho kev tivthaiv nyob rau hauv lub tsis pub yog potassium ion (K+) los ntawm KCl, uas txhawb nqa primer annealing.

Ua raws li qhov xav tau, PCR txoj kev yog dab tsi?

PCR ( polymerase saw cov tshuaj tiv thaiv ) yog a txoj kev txhawm rau txheeb xyuas qhov luv luv ntawm DNA (lossis RNA) txawm nyob rau hauv cov qauv uas tsuas muaj feeb ntau ntawm DNA lossis RNA. PCR yog siv los tsim dua tshiab (ua kom nrov) xaiv cov ntu ntawm DNA lossis RNA. PCR yog qhov ua tau zoo heev hauv qhov tsis paub tseeb ntawm cov ntawv luam tuaj yeem ua los ntawm DNA.

4 kauj ruam ntawm PCR yog dab tsi?

Cov kauj ruam koom nrog hauv Polymerase Chain Reaction hauv DNA Sequence

  • Kauj Ruam 1: Denaturation los ntawm Thaum tshav kub kub: Thaum tshav kub kub yog feem ntau ntau tshaj 90 degrees Celsius ntawm cais ob-stranded DNA rau hauv ob qho tib si strands.
  • Kauj Ruam 2: Annealing Primer rau Target Sequence:
  • Kauj Ruam 3: Extension:
  • Kauj Ruam 4: Xaus ntawm Thawj PGR voj voog:

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