Video: CM Sephadex yog dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Txheej txheem cej luam. CM Sephadex C-50 yog qhov tsis muaj zog cation exchanger raws li cov ntaub ntawv zoo thiab muaj pov thawj zoo Sephadex puag matrix. Tsis muaj zog cation exchanger haum batch txheej txheem. Siab khi muaj peev xwm.
Kuj paub, Sephadex g100 yog dab tsi?
Sephadex yog ib tug cross-linked dextran gel siv rau gel pom. Nws tau tsim los ntawm Pharmacia hauv xyoo 1959, tom qab kev txhim kho ua haujlwm los ntawm Jerker Porath thiab Per Flodin. Lub npe yog muab los ntawm kev sib cais Pharmacia dextran. Nws yog ib txwm tsim nyob rau hauv daim ntawv hlaws thiab feem ntau yog siv rau gel pom kab.
Tsis tas li ntawd, Sephadex g50 yog dab tsi? Sephadex G - 50 Superfine yog ib qho zoo tsim gel filtration resin rau desalting thiab tsis pauv biomolecules> 30 000 molecular hnyav. Superfine cov hlaws me me muab kev ua haujlwm ntau dua. Ceev ceev desalts, tshem tawm cov kab mob thiab hloov mus rau ib qho tshiab tsis nyob rau hauv ib kauj ruam.
Cov lus nug tseem yog, dab tsi yog qhov feem ntau ntawm Sephadex g100?
Ob peb hom Sephadex muaj tam sim no, txhua tus muaj tus yam ntxwv fractionation ntau yam . Qhov porous gel tshaj plaws, Sephadex G-200, yuav faib cov proteins hauv Mw ntau 4000-800000, whereas lub siab txwv rau Sephadex G-25 yog 5000.
DEAE Sephadex yog dab tsi?
DEAE - Sephadex yog ib qho zoo-them slurry uas yuav muaj electrostatic kev sib cuam tshuam nrog cov tsis zoo them atoms, ua rau lawv elute tom qab tshaj qhov zoo-them molecules nyob rau hauv cov qauv txaus siab. Qhov no yog cov txheej txheem sib cais siv dav los tshawb pom cov proteins tshwj xeeb, lossis cov enzymes hauv lub cev.
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