Poly A Tail tiv thaiv degradation li cas?
Poly A Tail tiv thaiv degradation li cas?

Video: Poly A Tail tiv thaiv degradation li cas?

Video: Poly A Tail tiv thaiv degradation li cas?
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Cov poly-A tail ua rau RNA molecule ruaj khov thiab tiv thaiv nws degradation . Tsis tas li ntawd, lub poly-A tail tso cai rau tus tub txib paub tab RNA molecule raug xa tawm los ntawm lub nucleus thiab txhais ua cov protein los ntawm ribosomes hauv cytoplasm.

Kuj nug, poly A tail ua dab tsi?

Muaj nuj nqi. Hauv nuclear polyadenylation , a poly(A) tail yog ntxiv rau RNA thaum kawg ntawm kev sau ntawv. Hauv mRNAs, lub poly (A) tail tiv thaiv mRNA molecule los ntawm enzymatic degradation nyob rau hauv lub cytoplasm thiab pab nyob rau hauv transcription termination, export ntawm mRNA los ntawm lub nucleus, thiab kev txhais lus.

Ib tug kuj yuav nug, puas yog poly A tail ntxiv tom qab nres codon? Feem ntau, poly (A) tails tsis tau txhais vim hais tias feem ntau mRNAs encode a nres codon uas xaus kev txhais lus thiab tiv thaiv cov ribosome kom ncav cuag 3' kawg ntawm cov lus.

Tsis tas li ntawd, poly A tail ntxiv rau pre mRNA Lub hom phiaj ntawm poly A Tail yog dab tsi?

PAP ntxiv kwv yees li 10 adenine nucleotides mus rau qhov kawg ntawm 3' ua ntej - mRNA molecule. Qhov sib ntxiv ntawm luv poly (A) tail tso cai rau kev khi ntawm cov poly (A) binding protein (PABII) to the qab . PABII nce tus nqi ntawm polyadenylation , uas tom qab tso cai rau ntau PABII protein los khi cov qab.

Koj yuav nruab poly A Tail li cas?

Cov poly (A) tail tuaj yeem nkag mus rau hauv cov qauv DNA los ntawm kev siv lub tailed PCR primer, lossis nws tuaj yeem muab ntxiv rau RNA los ntawm kev kho enzymatic nrog E. coli. Poly (A) Polymerase (NEB #M0276). Qhov ntev ntawm qhov ntxiv qab tuaj yeem hloov kho los ntawm titrating Poly (A) Polymerase hauv cov tshuaj tiv thaiv (Daim duab 6).

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