Video: Dab tsi yog qhov zoo electrode hauv electrophoresis?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Yog tsis muaj gel, tag nrho cov DNA yuav mus zoo zoo electrode (hu ua anode). Qhov loj ntawm qhov pores tswj tus nqi ntawm DNA txav. Ib qho tseem ceeb ntawm 1% agarose yog siv los cais cov DNA me me thaum cov concentrations qis dua yog siv los cais cov kab mob loj.
Ib yam li ntawd, puas yog qhov zoo lossis qhov tsis zoo electrode ze tshaj plaws rau lub qhov dej?
Thaum siv hluav taws xob tam sim no, pom tias qhov tsis zoo electrode yog nyob ze ntawm lub qhov dej , thiab cov zoo electrode yog nyob deb tshaj ntawm lub qhov dej.
Tsis tas li ntawd, vim li cas DNA txav mus rau qhov zoo electrode? Cov tsis zoo them rau cov qab zib-phosphate caj qaum ntawm DNA polymers ua rau lawv tsiv teb tsaws mus rau zoo electrode thaum muab tso rau hauv qhov hluav taws xob. Cov pores txwv qhov txav ntawm lub DNA thiab tsim ib puag ncig uas txhua tus neeg DNA fragment tus nqi ntawm kev txav mus los txawv raws li nws qhov ntev.
Ua li no, DNA puas tsiv mus rau qhov zoo lossis tsis zoo electrode thaum electrophoresis?
Gel electrophoresis thiab DNA DNA yog tsis zoo them, yog li ntawd, thaum muaj hluav taws xob tam sim no siv rau lub gel, DNA yuav tsiv teb tsaws mus rau qhov zoo them nqi electrode.
Vim li cas cov anode zoo hauv electrophoresis?
Cov khoom raug them tuaj yeem sib cais vim tias lawv tsiv mus rau qhov sib txawv ntawm cov gel. Hauv gel electrophoresis , cov zoo tus ncej anode thiab tus ncej tsis zoo hu ua cathode ; Yog li ntawd, cov nqi them yuav tsiv mus nyob rau cov nodes.
Pom zoo:
Dab tsi yog qhov txawv ntawm qhov pom tseeb qhov loj thiab qhov ntsuas qhov ntsuas qhov tseeb?
Dab tsi yog qhov txawv ntawm qhov pom tseeb thiab qhov tseeb? Pom meej qhov loj npaum li cas yog qhov ci ntawm lub hnub qub tshwm los ntawm lub ntiaj teb thiab nyob ntawm qhov ci thiab qhov deb ntawm lub hnub qub. Qhov loj npaum li cas yog qhov ci ntawm lub hnub qub yuav tshwm sim los ntawm tus qauv nyob deb
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Cov qauv DNA raug thauj mus rau hauv qhov dej ntawm qhov tsis zoo electrode kawg ntawm gel. Lub zog yog qhib thiab DNA tawg tawg los ntawm gel (mus rau qhov zoo electrode). Cov tawg loj tshaj plaws yog nyob ze rau sab saum toj ntawm cov gel (tsis zoo electrode, qhov chaw uas lawv pib), thiab cov khoom me me nyob ze rau hauv qab (zoo electrode)
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