UV spectrometer ua haujlwm li cas?
UV spectrometer ua haujlwm li cas?

Video: UV spectrometer ua haujlwm li cas?

Video: UV spectrometer ua haujlwm li cas?
Video: Yuav ua li cas kom yus lub hlwb ua haujlwm zoo 2024, Kaum ib hlis
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Hauv UV -Vis, ib lub teeb nrog lub wavelength sib txawv ntawm 180 thiab 1100 nm dhau los ntawm kev daws teeb meem hauv cuvette. Tus nqi ntawm lub teeb uas yog absorbed los ntawm cov tshuaj nyob ntawm qhov concentration, txoj kev ntev ntawm lub teeb los ntawm lub cuvette thiab zoo npaum li cas cov analyte lub teeb absorbs ntawm ib tug tej yam wavelength.

Hauv qhov no, spectrometer ua haujlwm li cas?

Basic function ntawm a spectrometer yog coj nyob rau hauv lub teeb, rhuav nws mus rau hauv nws spectral Cheebtsam, digitize lub teeb liab raws li ib tug muaj nuj nqi ntawm wavelength, thiab nyeem nws tawm thiab tso saib nyob rau hauv lub computer. Hauv feem ntau spectrometers , lub teeb divergent yog ces collimated los ntawm ib daim iav concave thiab coj mus rau ib tug grating.

Ib tug kuj yuav nug, UV spectrophotometer absorbance li cas? Siv UV-absorption spectra los nrhiav cov concentrations

  1. Koj yuav tsum nco ntsoov Beer-Lambert Txoj Cai:
  2. Cov lus qhia ntawm sab laug ntawm qhov sib npaug yog hu ua absorbance ntawm cov tshuaj thiab yog ntsuas los ntawm ib tug spectrometer.
  3. A = ϵ lc.
  4. Lub cim epsilon yog molar absorptivity ntawm cov tshuaj.

Hauv qhov no, UV spectroscopy qhia koj li cas?

UV - pom spectrometers ua tau siv los ntsuas qhov absorbance ntawm ultra violet los yog pom lub teeb los ntawm ib tug qauv, txawm nyob rau hauv ib lub wavelength los yog ua ib tug scan hla ib tug ntau yam ntawm cov spectrum . Cov UV thaj tsam thaj tsam li ntawm 190 txog 400 nm thiab pom thaj tsam ntawm 400 txog 800 nm.

Qhov tsawg kawg nkaus suav ntawm spectrometer yog dab tsi?

Txhawm rau txiav txim siab Tsawg kawg suav ntawm qhov Circular Vernier Scale. lub hauv paus ntsiab lus zoo ib yam li cov linear vernier nplai. Tag nrho lub voj voog yog muab faib ua 360 degrees. Ces, Tsawg kawg suav = s - v = s - (59/60)s = (1/60) s = 1/60 degree = 1 feeb.

Pom zoo: