Cov txheej txheem:
Video: Koj ntsuas tus nqi ntawm enzyme ua haujlwm li cas?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2024-01-18 08:15
Kev ntsuam xyuas enzyme
- Enzyme kev soj ntsuam yog cov txheej txheem kuaj rau ntsuas kev ua haujlwm enzymatic .
- Qhov ntau los yog concentration ntawm ib enzyme tuaj yeem nthuav tawm hauv cov nyiaj molar, xws li nrog rau lwm yam tshuaj, lossis hais txog kev ua si hauv enzyme units.
- Kev ua haujlwm enzyme = moles ntawm substrate hloov dua siab tshiab rau ib chav tsev lub sij hawm = tus nqi × cov tshuaj tiv thaiv ntim.
Raws li qhov no, koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme li cas?
Cov cov tshuaj tiv thaiv , kuj hu ua tshuaj tiv thaiv velocity, yog ntsuas ntawm kev tsim cov khoom raws sijhawm. Ib enzyme tus nqi ntawm tshuaj tiv thaiv feem ntau suav nrog kev sib npaug Michaelis-Menten, uas siv rau hauv tus account substrate thiab enzyme concentration, raws li zoo raws li ib tug substrate affinity rau ib enzyme , npe Km.
Ib sab saum toj no, koj ntsuas cov kev ua enzyme tshwj xeeb li cas? Yog li ntawd, yam tshwj xeeb yog xam los ntawm kev faib cov naj npawb ntawm units / mL los ntawm cov protein concentration hauv mg / mL kom tau Μmol / min / mg. Piv txwv li: Lub yam tshwj xeeb ntawm kev cais enzyme yog ntsuas ntawm 150 Μmoles / min / mg protein ua ntej purification thiab 800 Μmoles / min / mg, tom qab purification.
Raws li txoj cai, tus nqi ntawm kev ua enzyme yog dab tsi?
1.2. Qhov tshwj xeeb kev ua si ntawm ib enzyme yog qhia raws li tus naj npawb ntawm units ib milligram ntawm cov protein. Cov tus nqi ntawm biochemical cov tshuaj tiv thaiv ntawm qhov ntsuas kub thiab pH nyob ntawm qhov enzyme concentration thiab substrate concentration.
Dab tsi cuam tshuam rau kev ua haujlwm enzyme?
Ob peb yam cuam tshuam rau tus nqi ntawm cov tshuaj tiv thaiv enzymatic mus - kub , pH, enzyme concentration , substrate concentration , thiab muaj cov inhibitors lossis activators.
Pom zoo:
Yuav ua li cas koj pom qhov ntsuas qhov ntsuas ntawm dilation ntawm lub dav hlau ua haujlwm?
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Txhais li cas los ntawm tus nqi ntws ntawm tus nqi hluav taws xob?
Ib coulomb ib ob