Koj pom km hauv Lineweaver Burk li cas?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Lub Lineweaver-Burk Plot

1. y = 1/V.
2. x = 1/S.
3. m = K_M/V_max
4. b = 1/[S]
5. x-intercept = -1/K_M

Raws li, Lineweaver Burk tuaj yeem siv ob txoj kev sib koom ua ke los txiav txim siab km?

Cov ob npaug - kev sib raug zoo (tseem hu ua Lineweaver - Burk ) zaj yog tsim los ntawm phiaj xwm inverse pib tshaj tawm (1 / V₀) raws li afunction ntawm inverse ntawm lub substrate concentration (1 / [S]). TheV_max ua tau ua kom raug txiav txim siab thiab yog li ntawd K_M ua tau kuj be txiav txim siab nrog qhov tseeb vim tias txoj kab ncaj nraim yog tsim.

Qhov sib npaug Lineweaver Burk yog dab tsi? Cov Lineweaver - Burk kev sib npaug yog linear kev sib npaug , qhov twg 1/V yog linear muaj nuj nqi ntawm 1/[S] es tsis txhob V yog ib tug rational muaj nuj nqi ntawm [S]. Cov Lineweaver - Burkequation tuaj yeem sawv cev tau yooj yim los txiav txim siab qhov tseem ceeb ntawm K_m thiab V_max. Muab ib Lineweaver - Burk zaj, txiav txim K_m ntawm ib qho enzyme.

Ua raws li qhov no, vim li cas Lineweaver Burk zajlus tseem ceeb hauv enzyme kinetics?

Cov Lineweaver – Burk zaj duab xis tau dav siv los txiav txim tseem ceeb cov ntsiab lus hauv cov enzyme kinetics , xws li Km thiab Vmax, ua ntej muaj dav dav ntawm cov khoos phis tawj muaj zog thiab cov software tsis-linear regression. Nws kuj muab aquick, pom kev xav ntawm cov ntaub ntawv sib txawv ntawm enzyme inhibition.

Km thiab Vmax yog dab tsi?

Tus nqi ntawm cov tshuaj tiv thaiv thaum lub enzyme saturated nrog substrate yog qhov siab tshaj plaws ntawm cov tshuaj tiv thaiv, Vmax . Qhov no isusually expressed li tus Km (Michaelis tas li) ntawm cov enzyme, ib qho kev ntsuas kev sib hloov ntawm kev sib raug zoo. Rau kev siv, Km yog qhov concentration ntawm substrate uas tso cai rau cov enzyme kom ua tiav ib nrab Vmax.