Video: Dab tsi yog lentiviral plasmid?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Nrov Lentiviral Hloov Plasmids
Thaum twg lentivirus yog siv rau kev tshawb fawb, nws yog tus lentiviral genome uas encodes genetic khoom uas tus kws tshawb fawb xav tau xa mus rau cov hom phiaj tshwj xeeb. Qhov no genome yog encoded los ntawm plasmids hu ua "transfer plasmids , "uas tuaj yeem hloov kho kom nkag siab ntau yam ntawm cov khoom siv noob.
Tsis tas li ntawd, lub lentiviral vector ua haujlwm li cas?
Lentiviral vectors yog ib hom kab mob retrovirus uas tuaj yeem kis tau ob qho tib si sib faib thiab tsis sib cais vim tias lawv qhov kev sib koom ua ke ua ntej (tus kab mob "plhaub") tuaj yeem nkag mus los ntawm cov ntaub so ntswg ntawm lub hauv paus ntawm lub hom phiaj ntawm tes.
Kuj Paub, lub ntim plasmid yog dab tsi? Ib lossis ntau dua plasmids , feem ntau hu ua ntim plasmids , encode cov virion proteins, xws li lub capsid thiab rov qab transcriptase. Lwm tus plasmid muaj cov genetic khoom xa los ntawm vector. Cov kab ke no yog siv los ntim cov genome rau hauv virion.
Ib tug kuj yuav nug, lentiviral txhais li cas?
Lentivirus yog ib tsev neeg ntawm cov kab mob uas muaj lub luag haujlwm rau cov kab mob tseem ceeb xws li HIV, uas kis tau los ntawm kev tso DNA rau hauv lawv cov cell genome. Lentiviruses tuaj yeem dhau los ua endogenous (ERV), sib koom ua ke lawv cov genome rau hauv tus tswv tsev germline genome, kom tus kab mob no tau txais los ntawm tus tswv tsev cov xeeb ntxwv.
Dab tsi yog 2 vectors feem ntau siv?
Ob hom ntawm cov duab yog feem ntau siv : E. coli plasmid cov duab thiab bacteriophage λ cov duab . Plasmid cov duab replicate nrog rau lawv cov tswv cell, thaum λ cov duab replicate li lytic viruses, tua lub party cell thiab ntim cov DNA rau hauv virions (Tshooj 6).
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