Video: Yuav ua li cas cov khoom tsim tawm ib feeb cuam tshuam rau tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Rau ib enzyme catalyzed cov tshuaj tiv thaiv , cov tus nqi yog feem ntau qhia nyob rau hauv tus nqi ntawm cov khoom tsim tawm ib feeb . Thaum tsis tshua muaj kub, ua kom sov feem ntau nce qhov tus nqi ntawm ib qho enzyme catalyzed cov tshuaj tiv thaiv vim cov reactants muaj ntau zog, thiab ua tau yooj yim dua ua kom muaj zog theem.
Yog li, dab tsi tuaj yeem siv los txiav txim siab tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed?
Enzyme catalysis kuaj pom los ntawm kev ntsuas qhov pom ntawm cov khoom lossis ploj ntawm cov reactants. Rau ntsuas ib yam, koj yuav tsum muaj peev xwm pom nws. Enzyme kev ntsuam xyuas yog cov kev xeem tsim los rau ntsuas kev ua haujlwm enzyme los ntawm kev ntsuas qhov kev hloov pauv hauv qhov concentration ntawm cov khoom uas kuaj tau.
Kuj Paub, absorbance cuam tshuam li cas rau kev ua enzyme? Muab tso rau lwm txoj kev, tus nqi ntawm cov substrate hloov dua siab tshiab rau hauv cov khoom yog proportional rau enzyme concentration. Yog li ntawd, tus nqi ntawm uas lub absorbance kev hloov pauv rau kev daws teeb meem hauv ib qho enzyme catalyzed cov tshuaj tiv thaiv yog proportional rau tus nqi uas cov khoom raug tsim.
Tsis tas li ntawd, ua li cas ib qho enzyme nce tus nqi ntawm cov tshuaj tiv thaiv nws catalyzes?
Ib enzyme txo qhov kev hloov pauv dawb-zog (ΔG) ntawm cov tshuaj tiv thaiv nws catalyzes . Ib enzyme catalyzes a tshuaj tiv thaiv los ntawm kev txo qis EA, ua kom cov reactant molecules nqus lub zog txaus kom ncav cuag qhov hloov pauv hauv lub xeev txawm tias nyob rau nruab nrab kub.
Dab tsi 4 yam tuaj yeem cuam tshuam txoj kev enzymes ua haujlwm?
Ob peb yam cuam tshuam rau tus nqi ntawm cov tshuaj tiv thaiv enzymatic mus - kub , pH, enzyme concentration, substrate concentration, thiab muaj cov inhibitors lossis activators.
Pom zoo:
Yuav ua li cas ntxiv tus inhibitor cuam tshuam tus nqi ntawm cov tshuaj tiv thaiv?
Kev piav qhia: Los ntawm txhais, inhibitors qeeb cov tshuaj tiv thaiv. Yog li ntawd, yog tias koj yuav tsum ntxiv ib qho inhibitor rau cov tshuaj tiv thaiv, koj yuav ua rau tus nqi ntawm cov tshuaj tiv thaiv txo. Cov tshuaj no ceev cov tshuaj tiv thaiv, yog li nce cov tshuaj tiv thaiv
Yuav ua li cas ib nrab lub neej ntawm xoom txiav txim cov tshuaj tiv thaiv cuam tshuam rau nws tus nqi tas li?
Nyob rau hauv xoom-order kinetics, tus nqi ntawm cov tshuaj tiv thaiv tsis yog nyob ntawm seb lub substrate concentration. Tus qauv t 1/2 rau qhov kev txiav txim xoom qhia pom tias ib nrab-lub neej nyob ntawm tus nqi ntawm qhov pib concentration thiab tus nqi tas li
Koj ntsuas tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed li cas?
Enzyme catalysis yog kuaj pom los ntawm kev ntsuas qhov pom ntawm cov khoom lossis ploj ntawm cov reactants. Txhawm rau ntsuas qee yam, koj yuav tsum muaj peev xwm pom nws. Enzyme assay yog cov kev ntsuam xyuas tsim los ntsuas kev ua haujlwm ntawm enzyme los ntawm kev ntsuas qhov kev hloov pauv hauv cov ntsiab lus ntawm cov tshuaj uas pom tau
Dab tsi tuaj yeem siv los txiav txim siab tus nqi ntawm cov tshuaj tiv thaiv enzyme catalyzed?
Enzyme catalysis yog kuaj pom los ntawm kev ntsuas qhov pom ntawm cov khoom lossis ploj ntawm cov reactants. Txhawm rau ntsuas qee yam, koj yuav tsum muaj peev xwm pom nws. Enzyme assay yog cov kev ntsuam xyuas tsim los ntsuas kev ua haujlwm ntawm enzyme los ntawm kev ntsuas qhov kev hloov pauv hauv cov ntsiab lus ntawm cov tshuaj uas pom tau
Yam dab tsi cuam tshuam rau tus nqi ntawm cov tshuaj tiv thaiv tshuaj?
Cov yam ntxwv uas cuam tshuam rau cov tshuaj tiv thaiv yog: thaj tsam ntawm cov khoom reactant. concentration los yog siab ntawm ib tug reactant. kub. xwm ntawm cov reactants. muaj/tsis muaj catalyst