Video: Dab tsi yog qhov haujlwm ntawm kev sojntsuam hauv Southern blot?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Tom qab ntawd lub membrane raug kho nrog ib qho me me ntawm DNA lossis RNA hu ua a soj ntsuam , uas tau raug tsim los kom muaj ib theem zuj zus ntxiv rau ib qho DNA ib ntus hauv cov qauv; qhov no tso cai rau cov soj ntsuam mus hybridize, los yog khi, mus rau ib qho DNA feem ntawm daim nyias nyias.
Ntxiv mus, lub hom phiaj ntawm Southern blot quizlet yog dab tsi?
Siv rau kev txheeb xyuas homologous sequences hauv ib gel blot nruab nrab ntawm DNA / RNA lub hom phiaj thiab DNA "kev soj ntsuam."
Ib yam li ntawd, lub hom phiaj ntawm prehybridization yog dab tsi? Prehybridization (Kev thaiv) Salmon phev DNA feem ntau yog siv los ua tus neeg sawv cev thaiv kev tiv thaiv kev sojntsuam los ntawm kev ua raws li daim nyias nyias, kom ntseeg tau tias nws tsuas yog cuam tshuam nrog cov DNA xav tau uas tau xa mus rau daim nyias nyias.
Dhau li ntawm no, dab tsi yog daim ntawv thov ntawm Southern blotting?
Southern blotting yog tsim los nrhiav ib qhov sib lawv liag ntawm DNA nyob rau hauv ib tug complex sib tov. Piv txwv li, Southern Blotting tuaj yeem siv los nrhiav cov noob tshwj xeeb hauv tag nrho genome. Tus nqi ntawm DNA xav tau rau cov txheej txheem no yog nyob ntawm qhov loj thiab cov haujlwm tshwj xeeb ntawm kev sojntsuam.
Dab tsi yog qhov tseeb ntawm cov xwm txheej hauv Southern blotting?
Muaj plaub kauj ruam tseem ceeb hauv Southern blotting txheej txheem: Hauv thawj kauj ruam, qauv DNA tau tawg los yog zom rau hauv cov khoom me me uas siv cov enzyme txwv. Tom qab digestion, lub DNA Fragments raug cais siv gel electrophoresis. Agarose gel feem ntau yog siv rau lub hom phiaj no.
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