Video: Phenol red test rau dab tsi?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Phenol Liab Broth yog ib tug general-purpose sib txawv xeem nruab nrab feem ntau yog siv los sib txawv gram negative enteric kab mob. Nws muaj peptone, phenol liab (ib pH qhia), ib lub raj Durham, thiab ib qho carbohydrate (glucose, lactose, los yog sucrose).
Hauv qhov no, phenol liab lactose kuaj rau dab tsi?
Phenol Liab Lactose Nqaij qaib yog siv los kawm lactose fermentation nyob rau hauv kab mob ntau yam. Proteose peptone thiab nqaij nyug extract ua qhov chaw rau carbon thiab nitrogen. Sodium chloride yog osmotic stabilizer. Phenol liab yog qhov ntsuas pH, uas hloov daj ntawm acidic pH piv txwv li rau fermentation ntawm lactose.
Kuj Paub, nws txhais li cas thaum phenol liab hloov liab? Ib qho kev daws teeb meem ntawm phenol liab yog siv los ua pH ntsuas, feem ntau hauv kab lis kev cai ntawm tes. Nws cov xim nthuav tawm ib qho kev hloov pauv ntawm daj (λmax = 443 nm) ua liab (λmax = 570 nm) tshaj pH ntau 6.8 txog 8.2. Tshaj pH 8.2, phenol liab tig ib ci liab (fuchsia) xim.
Ntawm no, nws txhais li cas thaum phenol liab tig daj?
Phenol liab yog pH qhov taw qhia uas yog daj ntawm pH hauv qab 6.8 thiab liab ntawm pH saum toj no 7.4 nrog sib txawv ntxoov los ntawm daj rau liab nruab nrab ntawm cov qib pH. Yog tias qhov ntsuas tau tig daj hauv lub raj mis no txhais tau tias Nws tau dhau los ua paug nrog qee yam uas ua rau pH ntau acidic thiab coj pH qis dua 6.8.
Lub hom phiaj ntawm Durham raj hauv phenol liab kua zaub yog dab tsi?
Phenol Red Broth Base Control raj yog siv los ua kev tswj tsis zoo rau kev tshawb fawb fermentation. Lub raj Durham tuaj yeem muab tso rau hauv ib lub raj ntawm Phenol Red Broth w / Carbohydrate tso cai rau kev tshawb nrhiav cov khoom siv roj.
Pom zoo:
Cov xim ntawm phenol liab ntawm nruab nrab pH yog dab tsi?
Dab tsi yog xim ntawm phenol liab ntawm acid pH thiab ntawm alkaline pH? daj ntawm acid pH, ci liab ntawm alkaline pH. Phenol liab yog liab los yog txiv kab ntxwv nyob nruab nrab pH
Dab tsi xim yog phenol liab nyob rau hauv ib qho kev daws teeb meem?
Phenol liab yog cov kua qaub-puag qhia. Nws yog tsim los ntawm condensing ob moles ntawm phenol nrog ib tug mole ntawm o-sulfobenzoic acid anhydride. Phenol Liab yog siv los ua pH qhia hauv daim ntawv thov kab lis kev cai ntawm tes. Kev daws ntawm phenol liab yuav muaj xim daj ntawm pH ntawm 6.4 lossis qis dua thiab xim liab ntawm pH
Dab tsi yog acidic ethanol ntau dua los yog phenol?
Hauv phenol, rub cov pz electrons los ntawm oxygen atom mus rau hauv lub nplhaib ua rau cov hydrogen atom ua ib feem zoo dua li nws muaj nyob rau hauv aliphatic alcohols. Qhov no txhais tau tias nws yooj yim dua ploj los ntawm phenol dua li nws yog los ntawm aliphatic cawv, yog li phenol muaj cov khoom muaj zog acidic dua li ethanol
Dab tsi yog acidic phenol ntau dua ether?
Vim yog qhov delocalization ntawm cov nqi tsis zoo hauv lub nplhaib benzene, phenoxide ions ruaj khov dua li alkoxide ions. Yog li ntawd, peb tuaj yeem hais tias phenols yog acidic ntau dua li cawv
Dab tsi yog qhov tseem ceeb tshaj plaws uas yuav tsum muaj rau cov kua dej ntws hauv cov kav dej Dab tsi lwm yam cuam tshuam rau cov dej ntws?
Thaum ib qho kev quab yuam sab nraud yog exerted rau ntawm cov kua uas muaj, qhov tshwm sim siab yog kis sib npaug thoob plaws hauv cov kua. Yog li txhawm rau kom dej ntws, dej xav tau qhov sib txawv siab. Cov kav dej kuj tuaj yeem cuam tshuam los ntawm cov kua dej, cov yeeb nkab loj, qhov kub thiab txias (cov kav dej khov), cov kua dej ntom ntom