Vim li cas lub spectrophotometer yuav tsum tau teem rau ntawm ib qho kev wavelength?

2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36

Thaum koj kho cov wavelength ntawm a spectrophotometer , koj yog hloov txoj hauj lwm ntawm prism los yog diffraction grating thiaj li txawv wavelengths ntawm lub teeb yog taw qhia rau ntawm lub slit. Qhov me me ntawm qhov dav dav, qhov zoo dua lub peev xwm ntawm qhov ntsuas los daws ntau yam sib txuas.

Yog li ntawd, lub wavelength yuav ua li cas rau spectrophotometer?

Nyob ntawm qhov ntau ntawm lub teeb ntawm lub teeb, nws tuaj yeem muab faib ua ob hom: UV-pom pom spectrophotometer: siv lub teeb hla ultraviolet ntau yam (185 - 400 nm) thiab pom ntau yam (400 - 700nm ua ) ntawm electromagnetic radiation spectrum.

Ib yam li ntawd, vim li cas koj thiaj li yuav tsum ntsuas lub spectrometer txhua zaus koj hloov lub wavelength? Spectrophotometer yuav tsum tau calibrated tawm tsam ib tug dawb paug daws li ntawd uas Kev ntsuas tom qab nws tuaj yeem siv cov tshuaj dawb paug absorbance raws li kev siv xoom. Ib qho kev ntsuas ntawm lub peev xwm ntawm ib yam khoom los nqus lub teeb ntawm ib qho kev teev tseg wavelength.

ntawm lub wavelength twg yuav tsum tau nyeem?

Noj nyeem ntawv ntawm 5 nm ntu me ntsis ua ntej thiab tom qab qhov no wavelength . Piv txwv li, yog tias qhov siab tshaj plaws absorbance tau pom ntawm 450 nm, ces kom tau txais qhov tseeb dua nyeem ntawv ntawm λmax, noj absorbance nyeem ntawv 440, 445, 455 thiab 460 nm.

Vim li cas koj thiaj ntsuas qhov nqus ntawm cov tshuaj ntawm 550 nm?

Proteins teb nrog tooj liab ions hauv alkaline daws tsim ib lub violet-xim complex uas absorbs lub teeb ntawm 550nm ua . Yog li ntawd, los ntawm ntsuas Cov concentration ntawm cov complex, siv A550 ( Absorbance ntawm 550nm ua ), koj kuj yog ntsuas qhov concentration ntawm cov protein.