Video: Dab tsi wavelength yuav tsum tau siv hauv spectrophotometer?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
UV pom spectrophotometer : siv lub teeb hla lub ultraviolet ntau yam (185 - 400 nm) thiab pom ntau yam (400 - 700 nm) ntawm electromagnetic radiation spectrum. IR spectrophotometer : siv lub teeb hla infrared ntau yam (700 - 15000 nm) ntawm electromagnetic radiation spectrum.
Tsuas yog li ntawd, ntawm qhov wavelength twg yuav tsum tau nyeem?
Noj nyeem ntawv ntawm 5 nm ntu me ntsis ua ntej thiab tom qab qhov no wavelength . Piv txwv li, yog tias qhov siab tshaj plaws absorbance tau pom ntawm 450 nm, ces kom tau txais qhov tseeb dua nyeem ntawv ntawm λmax, noj absorbance nyeem ntawv 440, 445, 455 thiab 460 nm.
Tom qab ntawd, lo lus nug yog, koj yuav txiav txim siab lub wavelength ntawm spectrophotometer li cas? 1 Teb
- Kev ua haujlwm wavelength yog xaiv los ntawm kev tshuaj xyuas cov spectrogram A(λ).
- Qhov twg ν yog qhov zaus ntawm EM yoj, c qhov ceev ntawm lub teeb thiab h lub Plank tas li.
- Lub spectrophotometer ntsuas qhov transmittance T (qhov sib piv ntawm kis ϕ thiab xwm txheej flux ϕ0 qhia raws li lub hwj chim) ntawm lub teeb beam los ntawm cov qauv ntawm tes.
Ib yam li ntawd, tib neeg nug, dab tsi yuav yog qhov zoo tshaj plaws wavelength rau ntsuas qhov nqus?
Cov zoo tshaj wavelength vim 450nm wavelength ntawm qhov siab tshaj plaws absorbance los ntawm FeSCN2+(aq).
Vim li cas peb siv lub wavelength ntawm qhov siab tshaj plaws absorbance?
Qhov no wavelength yog tus yam ntxwv ntawm txhua qhov sib xyaw? Nws muab cov ntaub ntawv ntawm cov qauv hluav taws xob ntawm cov tshuaj ntsuam xyuas? Nws ua kom siab tshaj rhiab heev thiab txo qis deviations ntawm Beer Txoj Cai. Peb tuaj yeem txiav txim siab λmax los ntawm kev npaj nqus tau vs wavelength hauv daim duab.
Pom zoo:
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