Lub ntsiab lus twg yuav elute ua ntej hauv roj chromatography?
Lub ntsiab lus twg yuav elute ua ntej hauv roj chromatography?

Video: Lub ntsiab lus twg yuav elute ua ntej hauv roj chromatography?

Video: Lub ntsiab lus twg yuav elute ua ntej hauv roj chromatography?
Video: Nkauj Noog Hawj - Luag Tsis Paub, Luag Thiaj Tseem Khuv Xim ❤️❤️(Nkauj Tawm Tshiab 2023) 2024, Kaum ib hlis
Anonim

Raws li txoj cai ntawm tus ntiv tes xoo, cov khoom siv ntawd elutes firstis feem ntau cov compound nrog rau qhov qis tshaj kub kub. Lwm yam impotent hais txog kev xav xaj yog lub polarity ntawm cov kua uas yog coated rau sab hauv ntawm lub GC kem (the stationary phase).

Tsuas yog li ntawd, qhov chaw twg yuav elute thawj zaug hauv kab chromatography?

Polar compounds yuav cuam tshuam nrog cov silicamore muaj zog tshaj cov tsis-polar sawv daws yuav yuav los ntawm kem , los yog lus , tom qab tsis-polar tshuaj sib xyaw . Thaum ib qho qauv muaj tshuaj sib xyaw nrog cov polarity zoo sib xws, cov kev sib cais ntawm qhov ntawd ua tau ua me me thiab rov qab tau tag nrho koj cov qauv huv ua tau ua ib qho kev sib tw.

Tsis tas li ntawd, elution hauv roj chromatography yog dab tsi? Gas chromatography . Cov gaseous compoundsbeing analyzed cuam tshuam nrog cov phab ntsa ntawm kem, uas yog coated nrog ib tug nyob ruaj khov theem. Qhov no ua rau txhua qhov sib xyaw ua ke lus ntawm lub sijhawm sib txawv, hu ua lub sijhawm khaws cia ntawm qhov sib xyaw. Qhov sib piv ntawm lub sijhawm khaws cia yog dab tsi muab GC nws analytical usefulness.

Ib tug kuj yuav nug, dab tsi txiav txim siab qhov kev txiav txim ntawm elution hauv roj chromatography?

Elution kev txiav txim hauv gas - kua dej chromatography nyob ntawm ob yam: lub boiling point of the solutes, thiab kev sis raug zoo ntawm solutes thiab thestationary theem. Cov kev txiav txim ntawm elution Thaum sivpolydimethyl siloxane feem ntau ua raws li cov ntsiab lus kub ntawm cov solutes, nrog cov kua dej qis dua. eluting ua ntej.

GCMS tuaj yeem kuaj tau dab tsi?

GC-MS Nws yog dav siv rau kev tsom xam ntawm cov tebchaw uas suav nrog esters, fatty acids, cawv, aldehydes, terpenes thiab lwm yam. ntes thiab ntsuas cov kab mob los ntawm kev lwj lossis kev ua qias tuaj uas yuav ua rau muaj kev phom sij thiab uas feem ntau tswj hwm los ntawm tsoomfwv cov koomhaum, piv txwv li tshuaj tua kab.

Pom zoo: