Yuav ua li cas koj xam lub sij hawm tuav hauv roj chromatography?
Yuav ua li cas koj xam lub sij hawm tuav hauv roj chromatography?

Video: Yuav ua li cas koj xam lub sij hawm tuav hauv roj chromatography?

Video: Yuav ua li cas koj xam lub sij hawm tuav hauv roj chromatography?
Video: Tag Kis Tsis Muaj Koj - Lig Muas Nkauj Tawm Tshiab 2022 2024, Kaum ib hlis
Anonim

Cov lub sijhawm khaws cia yog sum sij hawm ib qho piv txwv tivthaiv siv nyob rau hauv theem mobile thiab tus nqi ntawm sij hawm nws siv nyob rau theem nyob ruaj khov. Cov tom kawg yog hu ua net lossis kho lub sijhawm khaws cia (tR'). Qhov kev sib raug zoo piav qhia khaws cia hauv chromatography (ob leeg gas thiab kua) yog: tR = tR' +t0.

Ntxiv rau qhov no, lub sijhawm khaws cia hauv roj chromatography yog dab tsi?

Lub sijhawm khaws cia (RT) yog qhov ntsuas qhov ntsuas sij hawm coj mus rau ib tug solute mus hla ib chromatography kem. Nws yog xam raws li tus sij hawm los ntawm kev txhaj tshuaj mus rau kev kuaj pom. RT rau ib qho kev sib txuas tsis raug kho raws li ntau yam tuaj yeem cuam tshuam nws txawm tias tib yam GC thiab kem siv.

Ib yam li ntawd, tus qauv cuam tshuam li cas rau lub sijhawm khaws cia? Lub sijhawm khaws cia nyob tsis tau tsuas yog nyob rau qauv ntawm qhov tshwj xeeb molecule, tab sis kuj tseem nyob rau hauv cov xwm txheej xws li qhov xwm txheej ntawm lub xov tooj ntawm tes thiab nyob ruaj khov, tus nqi ntws ntawm theem mobile, thiab qhov ntev ntawm kab chromatographic. Lub sijhawm khaws cia feem ntau yog tus yam ntxwv rau ib qho kev sib xyaw hauv ib qho kev sib cais.

Ib yam li ntawd, dab tsi cuam tshuam rau lub sijhawm khaws cia hauv roj chromatography?

Tej yam cuam tshuam rau lub sijhawm khaws cia . Cov lub sijhawm khaws cia nyob ntawm ntau yam : tsom xam tej yam kev mob, hom kab, kem dimension, degradation ntawm kem, hav zoov ntawm active ntsiab lus xws li kis kab mob. thiab lwm yam. Yog hais tias xam tus piv txwv paub, tag nrho cov peaks tshwm nyob rau luv luv sij hawm thaum koj txiav ib feem ntawm kab.

Lub sij hawm tuav kho yog dab tsi?

Cov kho lub sij hawm tuav yog cov sij hawm tus analyte siv nyob rau hauv kem tsis nyob rau theem. Nco ntsoov tias kab chromatography yog tsim los ntawm ib theem nyob ruaj khov ntxiv rau theem mobile. Cov qauv txuas ntxiv muab faib nruab nrab ntawm theem mobile thiab theem nyob ruaj khov.

Pom zoo: