Cov txheej txheem:

Vim li cas malachite ntsuab spore stained?
Vim li cas malachite ntsuab spore stained?

Video: Vim li cas malachite ntsuab spore stained?

Video: Vim li cas malachite ntsuab spore stained?
Video: Mus zawm hniav zoo li cas puas mob 2024, Kaum ib hlis
Anonim

Ib tug thawj stain ( malachite ntsuab ) yog siv rau stain cov endospores. Vim endospores tiv taus staining , cov malachite ntsuab yuav raug yuam mus rau hauv (ie, malachite ntsuab permeate cov spore phab ntsa) cov endospores los ntawm cua sov. vim li no, cov malachite ntsuab yaug yooj yim los ntawm cov noob qoob loo.)

Tsis tas li ntawd, vim li cas cov niam hlwb tsis tuaj yeem khaws malachite ntsuab stain?

Txij li thaum malachite ntsuab yog dej-soluble thiab tsis ua raws li qhov zoo cell , thiab txij thaum vegetative hlwb tau cuam tshuam los ntawm tshav kub, lub malachite ntsuab yaug yooj yim los ntawm vegetative hlwb , tso cai rau lawv nkag tau yooj yim nce lub counterstain.

Qhov thib ob, vim li cas Spore staining tseem ceeb? Cov endospore stain yog qhov txawv stain siv los pom cov kab mob endospores. Endospores yog tsim los ntawm ob peb genera ntawm cov kab mob, xws li Bacillus. Los ntawm kev tsim spores , cov kab mob tuaj yeem muaj sia nyob hauv kev ua phem. Spores yog resistant rau tshav kub, dessication, tshuaj, thiab hluav taws xob.

Tsis tas li cov lus nug yog, koj ua li cas malachite ntsuab rau Endospore staining?

Kev xav tau

  1. Malachite ntsuab 0.5% (qhov no yog thawj stain) - Nws tuaj yeem npaj tau yooj yim siv 0.5 grams malachite ntsuab nrog 100 ml dej.
  2. Kais / dej distilled (decolorizing tus neeg sawv cev)

Yog vim li cas spores tsis stained los ntawm grams txoj kev?

Hauv cov kab mob, endospores yog cov txheej txheem tiv thaiv siv kom muaj sia nyob huab cua, tab sis qhov kev tiv thaiv no ua rau lawv nyuaj rau stain siv li qub cov txheej txheem xws li yooj yim staining thiab Gram staining.

Pom zoo: