Video: Vim li cas cov spores tshwm ntsuab thaum lub cev ntawm lub cev zoo li liab?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Vim li cas puas cov spores tshwm ntsuab thaum lub cev ntawm tes zoo li liab nyob rau hauv lub tiav lawm Endospore stain? Cov spore tshwm ntsuab vim cua sov yuam tus spore coj nyob rau hauv cov xim dye, uas yog yooj yim yaug tawm yog hais tias tus cev cev.
Tsuas yog li ntawd, vim li cas endospores stain ntsuab?
Vim yog lawv cov tawv nqaij protein ua los ntawm keratin, spores yog heev resistant mus li qub staining cov txheej txheem. Qhov tseem ceeb stain hauv endospore stain txheej txheem, malachite ntsuab , yog tsav mus rau hauv cov hlwb nrog cua sov.
Dab tsi yog qhov ua haujlwm ntawm malachite ntsuab hauv spores staining? Ib tug thawj stain ( malachite ntsuab ) yog siv rau stain cov endospores. Vim endospores tiv taus staining , cov malachite ntsuab yuav raug yuam mus rau hauv (ie, malachite ntsuab permeate cov spore phab ntsa) cov endospores los ntawm cua sov. Nyob rau hauv cov txheej txheem no cua sov ua raws li ib tug mordant.
Tom qab ntawd, vim li cas cov stained endospores tshwm sim txawv ntawm cov noob qoob loo?
Tom qab ntxuav, tsuas yog lub endospores yuav khaws cov thawj stain Malachite ntsuab. Safranin yog ces siv los ua counterstain rau cov noob qoob loo . Cov endospores stain yog ib tug txawv stain vim nws txawv spore-formers los ntawm non spore-formers. Nco tseg: Tsim ib qho endospores.
Vim li cas cov smear yuav tsum tau steamed thaum thov malachite ntsuab rau cov swb?
Kub los yog steam yog siv tau Malachite Ntsuab mus rau hauv cov endospores (thiab cov vegetative hlwb ib yam nkaus. Tab sis ib hom uas tsim endospores muaj vegetative bacilli.) Cov swb yog lawv ntxuav nrog dej. Raws li Malachite Green yog Ib tug dej soluble stain, nws ntxuav tawm ntawm vegetative hlwb, tab sis tsis tawm ntawm lub endospores.
Pom zoo:
Vim li cas nws yog ib lub tswv yim zoo los cawm tag nrho cov txheej txheem ntawm koj cov extraction kom txog thaum kawg ntawm kev sim?
Kev ua yuam kev thaum lub sijhawm rho tawm (piv txwv li nqa nrog cov txheej txheem tsis raug), tuaj yeem daws tau yog tias cov kev daws tsis tau muab tso rau hauv lub thawv pov tseg! Cov khaubncaws sab nraud povtseg yuav tsum tau txais kev cawmdim kom txog thaum tom qab evaporation vim hais tias cov khoom xav tau yuav tsis soluble heev hauv cov kuab tshuaj siv
Vim li cas peb thiaj cia siab tias cov kab mob Gram tsis zoo yuav stain liab liab thaum lub sij hawm Gram staining txheej txheem?
Thaum cov kab mob gram zoo stain violet vim muaj cov txheej txheem peptidoglycan tuab nyob rau hauv cov phab ntsa ntawm lawv cov cell, cov kab mob gram tsis zoo stain liab, vim yog cov txheej peptidoglycan thinner hauv lawv cov phab ntsa ntawm tes (peptidoglycan txheej tuab dua tso cai rau cov kab mob gram zoo stains violet. tuav lub stain, tab sis ib txheej nyias
Thaum lub ntim ntawm ib qho piv txwv ntawm cov pa roj yog txo lub siab ntawm cov qauv ntawm cov pa roj?
Kev txo qis Cov pa roj sib xyaw txoj cai hais tias lub siab ntawm cov pa roj yog inversely cuam tshuam nrog lub ntim thiab ncaj qha ntsig txog qhov kub thiab txias. Yog tias qhov kub thiab txias yog nyob tas li, qhov sib npaug raug txo rau Boyle txoj cai. Yog li ntawd, yog tias koj txo qhov siab ntawm cov roj av tas li, nws qhov ntim yuav nce ntxiv
Yuav ua li cas tshwm sim thaum koj muab cov khoom them nqi ze ntawm Electroscope thiab vim li cas?
Hauv cov txheej txheem induction ntawm kev them nyiaj, ib qho khoom them raug coj los ze rau tab sis tsis kov lub electroscope. Qhov no tau piav qhia los ntawm cov kev them nqi zoo li lub hauv paus ntsiab lus. Lub zais pa uas tsis zoo tiv thaiv cov hluav taws xob tsis zoo, yog li yuam kom lawv txav mus los
Vim li cas cov kab mob Gram tsis zoo tshwm liab liab thaum cov kab mob Gram zoo pom ntshav liab?
Gram zoo hlwb stain ntshav vim lawv cov peptotidoglycan txheej yog tuab txaus, txhais tau tias tag nrho cov kab mob Gram zoo yuav khaws lawv cov stain. Gram tsis zoo hlwb stain liab vim lawv muaj ib tug nyias peptidoglycan phab ntsa, thiab lawv yuav tsis khaws ib yam ntawm cov ntshav stain los ntawm crystal violet