Video: Koj ua li cas TLC xeem?
2024 Tus sau: Miles Stephen | [email protected]. Kawg hloov kho: 2023-12-15 23:36
Txiav lub TLC daim ntawv rau hauv strips kwv yees li 2 cm x 7 cm. Kos ib kab xaum rau ntawm sab luv, kwv yees li 0.5 cm ntawm hauv qab. Ua Tsis txhob siv tus cwj mem raws li tus cwj mem yuav yaj hauv cov kuab tshuaj organic thiab cais tawm, ua rau tsis pom lossis ua rau koj cov txiaj ntsig. Ncuav cov kuab tshuaj los ua kuaj rau hauv lub khob ntim.
Cov lus nug tseem yog, vim li cas peb thiaj ua TLC xeem?
Nyias txheej chromatography , los yog TLC , yog ib txoj hauv kev los tshuaj xyuas cov khoom sib tov los ntawm kev sib cais cov sib xyaw ua ke hauv qhov sib tov. TLC tau siv los pab txiav txim seb tus naj npawb ntawm cov khoom nyob rau hauv ib tug sib tov, tus kheej ntawm cov tebchaw, thiab lub purity ntawm ib tug compound.
Ib yam li ntawd, dab tsi ua rau TLC hnyav hnyav? Solvent (Mob Phase) Tsim nyog kuab tshuaj kev xaiv yog tej zaum qhov tseem ceeb tshaj plaws ntawm TLC , thiab txiav txim qhov qhov hnyav tshaj plaws tej zaum yuav xav tau kev sim thiab ua yuam kev. Raws li nrog kev xaiv phaj, nco ntsoov cov tshuaj lom neeg ntawm cov analytes. Ib qho pib kuab tshuaj yog 1: 1 hexane: ethyl acetate.
Hauv qhov no, koj yuav txheeb xyuas qhov sib xyaw siv TLC li cas?
Siv nyias txheej chromatography rau txheeb xyuas cov tebchaw Ib qho me me ntawm qhov sib tov yog muab tso rau ntawm cov kab hauv qab ntawm cov phaj nyias nyias, thiab cov me me zoo sib xws ntawm cov amino acids paub tau muab tso rau ib sab. Lub phaj yog ces sawv hauv ib qho kuab tshuaj haum thiab sab laug los txhim kho zoo li ua ntej.
TLC txiav txim siab purity li cas?
Nyias Txheej Chromatography ( TLC ) yog cov txheej txheem sib cais uas xav tau cov qauv me me. Nws yog siv los ua txiav txim tus kev huv ntawm ib qho kev sib xyaw. Cov khoom ntshiab yuav pom tsuas yog ib qho chaw ntawm qhov tsim TLC phaj. Kev nce qib ntawm cov tshuaj tiv thaiv tuaj yeem soj ntsuam los ntawm Nyias Txheej Chromatography.
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