Koj yuav tsum teeb tsa koj lub thawv TLC li cas?
Koj yuav tsum teeb tsa koj lub thawv TLC li cas?

Video: Koj yuav tsum teeb tsa koj lub thawv TLC li cas?

Video: Koj yuav tsum teeb tsa koj lub thawv TLC li cas?
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Kev teeb tsa thiab khiav a TLC

Ib qho me me ntawm elutant yog nchuav rau hauv thawv (mus rau qhov tob txog 1/2 txog 2/3 ntawm tus hauv qab ntawm TLC daim ntawv-rau-pib kab nrug), thiab ib daim duab plaub ntawm cov ntawv lim tso kom nws kov tus elutant thiab dag rau lub thawv phab ntsa, feem ntau saum toj no tus laug laug.

Ib yam li ntawd, koj tuaj yeem nug, koj ua li cas TLC?

A TLC phaj yog ib daim ntawv ntawm iav, hlau, los yog yas uas yog coated nrog ib tug nyias txheej ntawm cov khoom adsorbent (feem ntau yog silica los yog alumina).

Thin Layer Chromatography (TLC)

  1. Kauj Ruam 1: Npaj lub thawv tsim.
  2. Kauj ruam 2: Npaj TLC phaj.
  3. Kauj ruam 3: Pom lub TLC phaj.
  4. Kauj Ruam 4: Txhim kho lub phaj.
  5. Kauj Ruam 5: Pom cov chaw.

Tsis tas li ntawd, TLC tuaj yeem txhim kho chromatography li cas? nrog kev sib cais ntawm ib hom preabsorbent nyias-txheej phaj. Cov cuab yeej pom tau tias muaj txiaj ntsig zoo hauv ntau txoj kev loj hlob chromatography . Kev daws teeb meem hauv nyias-txheej chromatography ( TLC ) tuaj yeem yog txhim kho los ntawm kev txo qhov loj ntawm qhov chaw coj mus kuaj thiab nce kev sib cais ntawm cov chaw tsim kho.

Tom qab ntawd, ib tus tuaj yeem nug, TLC phaj npaj li cas?

Kev npaj phaj Lawv yog npaj los ntawm kev sib xyaw cov adsorbent, xws li silica gel, nrog me me ntawm inert binder zoo li calcium sulfate (gypsum) thiab dej. Qhov sib tov no yog kis raws li cov slurry tuab ntawm ib daim ntawv tsis muaj peev xwm, feem ntau yog iav, tuab aluminium ntawv ci, los yog yas.

Yuav ua li cas yog tias cov kab hnyav nce mus txog saum koj lub phaj TLC?

Tshuaj txav mus a TLC phaj nrog rau kuab tshuaj tau siv los tsim cov phaj . Txawm li cas los xij, yog tias cov kuab tshuaj mus txog rau saum ntawm phaj , cov tshuaj txuas ntxiv mus.

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