Vim li cas peb thiaj siv qhov khoob hauv spectrophotometer?
Vim li cas peb thiaj siv qhov khoob hauv spectrophotometer?

Video: Vim li cas peb thiaj siv qhov khoob hauv spectrophotometer?

Video: Vim li cas peb thiaj siv qhov khoob hauv spectrophotometer?
Video: Tshuaj theem vim li cas thiaj tau nqi ?/ Siv kho mob dab tsi?/paris polyphylla use for? 2024, Kaum ib hlis
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A dawb paug kub yog siv mus calibrate spectrophotometer nyeem: lawv sau cov ntsiab lus teb ntawm ib puag ncig-cov cuab yeej-cov qauv qauv. Nws yog analogous rau "zeroing" ib teev ua ntej hnyav. Khiav dawb paug tso cai koj los sau cov kev cuam tshuam ntawm cov cuab yeej tshwj xeeb ntawm koj cov ntawv nyeem.

Yog li, lub hom phiaj ntawm qhov khoob hauv spectrophotometric yog dab tsi?

Ib ' dawb paug 'xov in spectrophotometric orany lwm daim ntawv ntawm analytical chemistry yog siv los txiav txim siab npaum li cas, yog tias muaj, cov txiaj ntsig ntawm qhov tshwm sim yog pab los ntawm cov khoom siv tsis yog cov khoom raug kuaj - piv txwv li, cov kuab tshuaj, thiab lwm yam.

Ib tug kuj yuav nug, dab tsi yog lub hom phiaj ntawm spectrometer? A spectrometer yog ib qho cuab yeej siv los soj ntsuam qhov teeb pom kev zoo li a muaj nuj nqi ntawm nws feem ntawm theelectromagnetic spectrum, feem ntau nws wavelength, zaus, orenergy. Lub spectroscope yog ib yam khoom uas ntsuas lub spectrum ntawm lub teeb.

Ua raws li qhov xav tau, lub hom phiaj ntawm qhov khoob hauv UV VIS spectrophotometer yog dab tsi?

A dawb paug yog ib qho piv txwv uas muaj txhua yam tshwj tsis yog rau kev txheeb xyuas ntawm kev txaus siab. Piv txwv li, yog tias koj tab tom ua a UV - vis sim ntsuas qhov concentration ntawm GreenFluorescent Protein, cov protein yuav tsum tau yaj hauv asolvent.

Tus qauv dawb yog dab tsi?

A reagent dawb hais txog qhov zoo me me errorin kuaj tau los ntawm cov reagents lawv tus kheej. A qauv dawb hais txog kev siv cov qauv rau zeroingan ntsuas thaum lub sij hawm xeem txheej txheem. A qauv dawb cancorrect rau tej zaum yuam kev los ntawm cov uas twb muaj lawm xim los yog turbidity nyob rau hauv lub qauv ua ntej reagents ntxiv.

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